SEMEN COLLECTION IN RAMS STANDING UP OR IN LATERAL RECUMBENCY USING DIFFERENT ELECTROEJACULATORS

Authors

  • Ligia Freitas de Lima Universidade de Brasília
  • Rodrigo Dorneles Tortorella Universidade de Brasília
  • Diogo Ramos Leal Universidade de Brasília
  • Pedro Ivo Braga Passos Universidade de Brasília
  • Jairo Pereira Neves Universidade de Brasília
  • Roberto Sartori Empresa Brasileira de Pesquisa Agropecuária, Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia
  • Thiago Antônio de Souza Nascimento Silva Universidade de Brasília
  • Hetielle Harumi Hashimoto Universidade de Brasília

DOI:

https://doi.org/10.5216/cab.v11i2.4009

Keywords:

Animal Reproduction

Abstract

The objective of the experiment was to evaluate and compare two electroejaculators: an automatic one (A – Biocom Ltda., Uberaba, MG) and a manual one (M – Duboi, Campo Grande, MS), using two methods of physical restrain: standing (E) or lateral recumbency (D). Twelve rams were used: Santa Inês (n=9), Ile-de-France (n=2) and Texel (n=1). Semen were collected twice a week and each animal had the semen collected three times in each group (AD – automatic in recumbency, AE – automatic in station, MD – manual in recumbency, and ME – manual in station) resulting in 12 collections. Blood samples were obtained before and after the semen collections to measure changes in circulating cortisol in order to measure stress among groups and individuals. The collections were evaluated per the time in seconds to obtain seminal fraction, time of seminal fraction and percentage of rams that exposed the penis. The collected semen parameters evaluated were: volume, aspect, progressive motility, wave motion, sperm vigor, total number of spermatozoa and sperm morphology. In 7.1% of the collections, there was no ejaculation, moreover, group AD was responsible for most of the collections without ejaculation (20%) (P<0,05). There was no difference among groups (P>0.05) relating to cortisol level after collections. However, there was difference among individuals (P<0.05). The collections in lateral recumbency were faster regarding achievement of seminal fraction and provided more time length for seminal fraction. The MD group required a shorter time length to achieve the seminal fraction and produced a greater volume of ejaculate in comparison to the AS group. It can be concluded by these results that both eletroejaculators (automatic and manual) had similar results as well as both methods of physical restrain (standing and lateral recumbency).

KEYWORDS: Animal welfare, cortisol, electroejaculation, semen, stress.

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Author Biographies

Ligia Freitas de Lima, Universidade de Brasília

Mestranda em Ciências Agrárias, Faculdade de Agronomia e  Medicina Veterinária, Universidade de Brasília

Rodrigo Dorneles Tortorella, Universidade de Brasília

Estudante graduação, Faculdade de Agronomia e Medicina Veterinária, UnB

Diogo Ramos Leal, Universidade de Brasília

Estudante de Graduação, Faculdade de Agronomia e Medicina Veterinária, UnB.

Pedro Ivo Braga Passos, Universidade de Brasília

Estudante de Graduação, Faculdade de Agronomia e Medicina Veterinária, UnB.

Jairo Pereira Neves, Universidade de Brasília

Professor Adjunto, Faculdade de Agronomia e Medicina Veterinária, UnB

Roberto Sartori, Empresa Brasileira de Pesquisa Agropecuária, Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia

Pesquisador da Embrapa, Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia

Thiago Antônio de Souza Nascimento Silva, Universidade de Brasília

Mestre em Ciências Agrárias, Faculdade de Agronomia e Medicina Veterinária, UnB.

Hetielle Harumi Hashimoto, Universidade de Brasília

Estudante de Graduação, Faculdade de Agronomia e Medicina Veterinária, UnB.

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Published

2010-06-23

How to Cite

DE LIMA, L. F.; TORTORELLA, R. D.; LEAL, D. R.; BRAGA PASSOS, P. I.; NEVES, J. P.; SARTORI, R.; NASCIMENTO SILVA, T. A. de S.; HASHIMOTO, H. H. SEMEN COLLECTION IN RAMS STANDING UP OR IN LATERAL RECUMBENCY USING DIFFERENT ELECTROEJACULATORS. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 11, n. 2, p. 410–416, 2010. DOI: 10.5216/cab.v11i2.4009. Disponível em: https://revistas.ufg.br/vet/article/view/4009. Acesso em: 22 nov. 2024.

Issue

Vol. 11 No. 2 (2010)

Section

Veterinary Medicine

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