PREVALENCE OF SHIGA TOXIN-PRODUCING Escherichia coli IN SOUTHERN BRAZIL ISOLATED FROM GROUND BEEF AND RAW MILK

Authors

  • Cláudio Dias Timm Universidade Federal de Pelotas
  • Fabrício Rochedo Conceição
  • Angelo Menin
  • Rita Santos Conceição
  • Odir Dellagostin
  • José Guimarães Aleixo

DOI:

https://doi.org/10.5216/cab.v10i2.4008

Keywords:

Shiga toxin-producing Escherichia coli, ground beef, milk, acid, ethanol

Abstract

The aim of this work was to investigate the occurrence of shiga toxin-producing Escherichia coli (STEC) in ground beef and raw milk in Southern Brazil, and to study the fate of STEC isolates from cattle faeces in ground beef and milk, and its resistance in acid and alcoholic media. Among 464 E. coli isolated from ground beef and raw milk there were no stx1 and stx2 genes. The population of STEC isolates from cattle faeces was quite stable when inoculated in ground beef and increased in inoculated milk along the 120 hours of storage at 8oC.  These STEC isolates were inactivated when exposed to pH 2.5 and 3.0, but they were viable after eight hours at pH 4.0. The STEC isolates did not survive 48 hours in medium containing 12% ethanol. At 6% ethanol, STEC O174:H21, O163:H19 and O112:H2 have shown an increase in population and STEC O91:H21 and O22:H8 did not resist beyond 24 and 48 hours of incubation, respectively. The low prevalence of STEC in foods together with the attributes of the STEC found in Brazilian cattle could be among the reasons for the low prevalence of foodborne diseases caused by STEC in Brazil.

KEY WORDS: Acid, ethanol, ground beef, milk, Shiga toxin-producing Escherichia coli.

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Author Biography

Cláudio Dias Timm, Universidade Federal de Pelotas

Inspeção de Leite e Derivados Departamento de Veterinária Preventiva Faculdade de Veterinária

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Published

2009-07-03

How to Cite

TIMM, C. D.; CONCEIÇÃO, F. R.; MENIN, A.; CONCEIÇÃO, R. S.; DELLAGOSTIN, O.; ALEIXO, J. G. PREVALENCE OF SHIGA TOXIN-PRODUCING Escherichia coli IN SOUTHERN BRAZIL ISOLATED FROM GROUND BEEF AND RAW MILK. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 10, n. 2, p. 641–649, 2009. DOI: 10.5216/cab.v10i2.4008. Disponível em: https://revistas.ufg.br/vet/article/view/4008. Acesso em: 17 jul. 2024.

Issue

Vol. 10 No. 2 (2009)

Section

Veterinary Medicine

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