ASSOCIATION BETWEEN THE PRESENCE OF A 38 kDa FACTOR IN THE SEMINAL PLASMA AND INHIBITION OF SPERM MOTILITY IN JUNDIÁ FISH Rhamdia quelen

Authors

  • Vinicius Farias Campos Universidade Federal de Pelotas
  • Tiago Collares Universidade Federal de Pelotas
  • Fabiana Kömmling Seixas Universidade Federal de Pelotas
  • Cristian Kaefer Universidade Federal de Pelotas
  • Paulo Varoni Cavalcanti Universidade de São Paulo
  • Marta Gonçalves Amaral Universidade Federal de Pelotas
  • Thomaz Lucia Jr. Universidade Federal de Pelotas
  • João Carlos Deschamps Universidade Federal de Pelotas

DOI:

https://doi.org/10.5216/cab.v11i2.3781

Keywords:

Animal Reproduction

Abstract

Protein factors have been identified in the seminal plasma of fish and mammal species and, in some situations, associated to sperm quality indicators. However, for jundiá fish (Rhamdia quelen), such factors and those potential associations remain unknown. In the present study, we aimed to identify some protein factors present in the seminal plasma of jundiá fish and to evaluate their association to sperm motility. SDS-PAGE was used to identify 14 bands, with molecular weight ranging from 217.1 to 7.1 kDa. Sperm motility was evaluated for 21 males. Four protein bands (81.5; 60.4; 33.6; and 25.5 kDa) were present in all seminal plasma samples. One protein band with molecular weight of 38.3 kDa was associated to reduced sperm motility of jundiá (P<0.01), since it was detected in 91.4% of the samples having motility lower than 80%. These results suggest that this seminal protein band associated to lower sperm motility may be considered a potential biochemical marker for sperm quality.

Keywords: protein, Rhamdia quelen, SDS-PAGE, seminal plasma, sperm motility.


Downloads

Download data is not yet available.

Author Biographies

Vinicius Farias Campos, Universidade Federal de Pelotas

Aluno do Programa de Pós-Graduação em Biotecnologia da UFPel, Centro de Biotecnologia.

Tiago Collares, Universidade Federal de Pelotas

Professor e Pesquisador do Centro de Biotecnologia da UFPel

Fabiana Kömmling Seixas, Universidade Federal de Pelotas

Professora e Pesquisadora do Centro de Biotecnologia da UFPel

Cristian Kaefer, Universidade Federal de Pelotas

Aluno do curso de Ciências Biológicas da UFPel e Bolsista de Iniciação Científica do Centro de Biotecnologia.

Paulo Varoni Cavalcanti, Universidade de São Paulo

Aluno do Programa de Pós-Graduação em Reprodução Animal da USP.

Marta Gonçalves Amaral, Universidade Federal de Pelotas

Professora do Departamento de Morfologia do Instituto de Biologia e Aluna do Programa de Pós-Graduação em Biotecnologia da UFPel

Thomaz Lucia Jr., Universidade Federal de Pelotas

Professor do Departamento de Patologia da Faculdade de Veterianária da UFPel e Pesquisador do Centro de Biotecnologia da UFPel

João Carlos Deschamps, Universidade Federal de Pelotas

Professor do Departamento de Patologia da Faculdade de Veterianária da UFPel e Pesquisador do Centro de Biotecnologia da UFPel

Downloads

Published

2010-06-23

How to Cite

CAMPOS, V. F.; COLLARES, T.; SEIXAS, F. K.; KAEFER, C.; CAVALCANTI, P. V.; AMARAL, M. G.; LUCIA JR., T.; DESCHAMPS, J. C. ASSOCIATION BETWEEN THE PRESENCE OF A 38 kDa FACTOR IN THE SEMINAL PLASMA AND INHIBITION OF SPERM MOTILITY IN JUNDIÁ FISH Rhamdia quelen. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 11, n. 2, p. 402–409, 2010. DOI: 10.5216/cab.v11i2.3781. Disponível em: https://revistas.ufg.br/vet/article/view/3781. Acesso em: 22 nov. 2024.

Issue

Vol. 11 No. 2 (2010)

Section

Veterinary Medicine

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).