ASSOCIATION BETWEEN THE PRESENCE OF A 38 kDa FACTOR IN THE SEMINAL PLASMA AND INHIBITION OF SPERM MOTILITY IN JUNDIÁ FISH Rhamdia quelen

Authors

  • Vinicius Farias Campos Universidade Federal de Pelotas
  • Tiago Collares Universidade Federal de Pelotas
  • Fabiana Kömmling Seixas Universidade Federal de Pelotas
  • Cristian Kaefer Universidade Federal de Pelotas
  • Paulo Varoni Cavalcanti Universidade de São Paulo
  • Marta Gonçalves Amaral Universidade Federal de Pelotas
  • Thomaz Lucia Jr. Universidade Federal de Pelotas
  • João Carlos Deschamps Universidade Federal de Pelotas

DOI:

https://doi.org/10.5216/cab.v11i2.3781

Keywords:

Animal Reproduction

Abstract

Protein factors have been identified in the seminal plasma of fish and mammal species and, in some situations, associated to sperm quality indicators. However, for jundiá fish (Rhamdia quelen), such factors and those potential associations remain unknown. In the present study, we aimed to identify some protein factors present in the seminal plasma of jundiá fish and to evaluate their association to sperm motility. SDS-PAGE was used to identify 14 bands, with molecular weight ranging from 217.1 to 7.1 kDa. Sperm motility was evaluated for 21 males. Four protein bands (81.5; 60.4; 33.6; and 25.5 kDa) were present in all seminal plasma samples. One protein band with molecular weight of 38.3 kDa was associated to reduced sperm motility of jundiá (P<0.01), since it was detected in 91.4% of the samples having motility lower than 80%. These results suggest that this seminal protein band associated to lower sperm motility may be considered a potential biochemical marker for sperm quality.

Keywords: protein, Rhamdia quelen, SDS-PAGE, seminal plasma, sperm motility.


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Author Biographies

Vinicius Farias Campos, Universidade Federal de Pelotas

Aluno do Programa de Pós-Graduação em Biotecnologia da UFPel, Centro de Biotecnologia.

Tiago Collares, Universidade Federal de Pelotas

Professor e Pesquisador do Centro de Biotecnologia da UFPel

Fabiana Kömmling Seixas, Universidade Federal de Pelotas

Professora e Pesquisadora do Centro de Biotecnologia da UFPel

Cristian Kaefer, Universidade Federal de Pelotas

Aluno do curso de Ciências Biológicas da UFPel e Bolsista de Iniciação Científica do Centro de Biotecnologia.

Paulo Varoni Cavalcanti, Universidade de São Paulo

Aluno do Programa de Pós-Graduação em Reprodução Animal da USP.

Marta Gonçalves Amaral, Universidade Federal de Pelotas

Professora do Departamento de Morfologia do Instituto de Biologia e Aluna do Programa de Pós-Graduação em Biotecnologia da UFPel

Thomaz Lucia Jr., Universidade Federal de Pelotas

Professor do Departamento de Patologia da Faculdade de Veterianária da UFPel e Pesquisador do Centro de Biotecnologia da UFPel

João Carlos Deschamps, Universidade Federal de Pelotas

Professor do Departamento de Patologia da Faculdade de Veterianária da UFPel e Pesquisador do Centro de Biotecnologia da UFPel

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Published

2010-06-23

How to Cite

CAMPOS, V. F.; COLLARES, T.; SEIXAS, F. K.; KAEFER, C.; CAVALCANTI, P. V.; AMARAL, M. G.; LUCIA JR., T.; DESCHAMPS, J. C. ASSOCIATION BETWEEN THE PRESENCE OF A 38 kDa FACTOR IN THE SEMINAL PLASMA AND INHIBITION OF SPERM MOTILITY IN JUNDIÁ FISH Rhamdia quelen. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 11, n. 2, p. 402–409, 2010. DOI: 10.5216/cab.v11i2.3781. Disponível em: https://revistas.ufg.br/vet/article/view/3781. Acesso em: 27 jul. 2024.

Issue

Vol. 11 No. 2 (2010)

Section

Veterinary Medicine

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