INFLUENCE OF IN VITRO FERTILIZATION TEMPERATURE AND THE Cumulus oophorus CELLS ON THE POLYSPERMY AND EMBRYO DEVELOPMENT RATES

Authors

  • Melissa Savoia Castilho Cunha
  • Mariana Groke Marques Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo
  • Alecsandra Sobreira Lima
  • Alessandra Corallo Nicacio
  • Mayra Elena Orrtiz Davila Assumpção
  • José Antonio Visintin

DOI:

https://doi.org/10.5216/cab.v11i2.3402

Keywords:

Animal Production

Abstract

Effects of in vitro fertilization (IVF) temperature and cumulus oophorus cells removal after IVF or 12 h of embryo culture (IVC) on polyspermy and embryo development rates were evaluated in swine. Oocytes and spermatozoa were incubated at 37 or 38.5ºC during IVF procedure. Cumulus oophorus cells were removed from 50% of zygotes of each group 8 hours after IVF and all zygotes were cultured with NCSU23 media. Polyspermy rates were assessed after 12 hours of IVC, when cumulus oophorus cells were removed from the rest of zygotes. In a second experiment, embryos remained in culture for the evaluation of embryo development. No effects of IVF temperature or cumulus oophorus cells removal were observed after 12 hours of IVC on polyspermy and embryo development (p<0.05). In conclusion, IVF temperature and the presence of cumulus oophorus cells after IVF do not interfere on polyspermy and embryo development rates.

 KEYWORDS: Cumulus oophorus cells, in vitro fertilization, polyspermy, swine, temperature.

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Author Biography

José Antonio Visintin

Laboratório de fecundação in vitro clonagem e transgenia animal. Departamento de Reprodução Animal, Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo.Rua Prof. Orlando Marques de Paiva, nº 85, São Paulo – SP, CEP 005588-270.mgroke@usp.br

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Published

2010-06-22

How to Cite

CUNHA, M. S. C.; MARQUES, M. G.; LIMA, A. S.; NICACIO, A. C.; ASSUMPÇÃO, M. E. O. D.; VISINTIN, J. A. INFLUENCE OF IN VITRO FERTILIZATION TEMPERATURE AND THE Cumulus oophorus CELLS ON THE POLYSPERMY AND EMBRYO DEVELOPMENT RATES. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 11, n. 2, p. 301–306, 2010. DOI: 10.5216/cab.v11i2.3402. Disponível em: https://revistas.ufg.br/vet/article/view/3402. Acesso em: 18 may. 2024.

Issue

Vol. 11 No. 2 (2010)

Section

Animal Production

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