Caracterização filogenética molecular e resistência antimicrobiana de Escherichia coli isoladas de caprinos neonatos com diarreia

Rodrigo Almeida Guimarães; Domingos Fernandes Lugo Neto; Mauro de Mesquita Souza Saraiva; Ricardo Pereira Lima; Mercia Rodrigues Barros; Mateus Matiuzzi da Costa; Celso Bruno Oliveira; Danilo Tancler Stipp

doi:10.1590/1089-6891v16i433639

MOLECULAR-PHYLOGENETIC CHARACTERIZATION AND ANTIMICROBIAL RESISTANCE OF Escherichia coli ISOLATED FROM GOATS WITH DIARRHEA

Authors

Rodrigo Almeida Guimarães Universidade Federal da Paraíba (UFPB), Areia, Paraíba, Brasil
Domingos Fernandes Lugo Neto Universidade Federal da Paraíba (UFPB), Areia, Paraíba, Brasil
Mauro de Mesquita Souza Saraiva Universidade Federal da Paraíba (UFPB), Areia, Paraíba, Brasil
Ricardo Pereira Lima Universidade Federal da Paraíba (UFPB), Areia, Paraíba, Brasil
Mercia Rodrigues Barros Universidade Federal Rural de Pernambuco (UFRPE), Recife, Pernambuco, Brasil
Mateus Matiuzzi da Costa Universidade Federal do Vale do São Francisco (UNIVASF), Petrolina, Pernambuco, Brasil
Celso Bruno Oliveira Universidade Federal da Paraíba (UFPB), Areia, Paraíba, Brasil
Danilo Tancler Stipp Universidade Federal da Paraíba (UFPB), Areia, Paraíba, Brasil

DOI:

https://doi.org/10.1590/1089-6891v16i433639

Keywords:

Parasithology

Abstract

Neonatal diarrhea determines significant changes in feed conversion, causing productivity loss in caprine herds. The antimicrobial resistance in bacteria is characterized as an important public healthissue; therefore, Escherichia coli may be characterized as an important pathogen due to expressingvirulence mechanisms responsible for significant clinical conditions in humans and animals. Thepresent study evaluated the presence of E. coli among 117 caprine fecal samples and analyzed theisolates for antimicrobial resistance. Suggestive colonies were submitted to biochemical screeningfollowed by genotypic group determination and phylogenetic analysis; further, the sampleswere submitted to antimicrobials susceptibility test. E. coli, Salmonella spp, Shigella sonnei andEnterobacter aerogenes were identified. E. coli isolates were phylogenetically classified as B2 (9/39),D (19/39), B1 (7/39) e A (4/29) groups. The analysis of the isolates also revealed the presence of K99(04/39) and Stx (02/39) virulence factors. Antimicrobial susceptibility test revealed sensitive isolatesto Chloramphenicol, Streptomycin, Amoxicillin and Ciprofloxacin, being all resistant to Lincomycin,Vancomycin and Penicillin. The results support the need of establishing restricted protocols forantimicrobial use, a fundamental procedure for health improvement in Brazilian caprine herds.

Keywords: antibiotics; bacteria; caprine; E. Coli.

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Published

2015-10-30

How to Cite

GUIMARÃES, Rodrigo Almeida; LUGO NETO, Domingos Fernandes; SARAIVA, Mauro de Mesquita Souza; LIMA, Ricardo Pereira; BARROS, Mercia Rodrigues; DA COSTA, Mateus Matiuzzi; OLIVEIRA, Celso Bruno; STIPP, Danilo Tancler. MOLECULAR-PHYLOGENETIC CHARACTERIZATION AND ANTIMICROBIAL RESISTANCE OF Escherichia coli ISOLATED FROM GOATS WITH DIARRHEA. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 16, n. 4, p. 615–622, 2015. DOI: 10.1590/1089-6891v16i433639. Disponível em: https://revistas.ufg.br/vet/article/view/33639. Acesso em: 28 dec. 2025.

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Issue

Vol. 16 No. 4 (2015)

Section

Veterinary Medicine

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