ADDITION OF SEMINAL PLASMA TO FROZEN-THAWED RAM SEMEN
DOI:
https://doi.org/10.5216/cab.v12i2.3344Keywords:
Animal reproductionAbstract
The considerable variability in constituents of seminal plasma could explain the particular differences in responses to freezing and in fertility rates after artificial insemination in ovines. This study assessed the effect of a pool of concentrated seminal plasma on ovine semen after thawing. Ejaculates of 12 Santa Inês rams were collected using an artificial vagina, diluted in Tris/egg yolk /glycerol and frozen in a semen and embryo freezer. After thawing, sperms were incubated using concentrated seminal plasma (10X) for 15 min. Semen was submitted to biochemical analysis, followed by a computerized assessment of its physical characteristics, hypoosmotic test and heat resistance evaluation. The electrophoretic analysis of seminal plasma indicated particular differences in the samples protein composition, which were attenuated by the seminal plasma pool. The samples treated with seminal plasma showed progressive reduced motility. No statistically significant difference was observed in the other semen characteristics (p<0.05), between samples treated with seminal plasma and control samples. The concentrated seminal plasma pool did not improve the assessed semen characteristics.
KEYWORDS: Rams; Freezing; Semen; Electrophoresis
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