HUMAN FOLLICLE STIMULATING HORMONE (hFSH) AND THYROXINE (T4) IN SURVIVAL MAINTENANCE AND IN VITRO GROWTH PROMOTION OF CAPRINE PREANTRAL FOLLICLES

Authors

  • Sanely Lourenço da Costa Universidade Federal de Viçosa/Doutora em Reprodução Animal
  • Eduardo Paulino da Costa Universidade Federal de Viçosa/Professor
  • Emílio César Martins Pereira Universidade Estadual Paulista Júlio Mesquita Filho/ Doutorado
  • Wagner Gonzaga Gonçalves Universidade Federal de Viçosa/Doutorado
  • Talita Fernandes da Silva Universidade Federal de Viçosa/Mestrado
  • Vanessa Lopes Dias Queiroz Universidade Federal de Viçosa / Mestrado

DOI:

https://doi.org/10.1590/cab.v16i2.31471

Keywords:

Activation, caprine, preantral follicles, hFSH, T4

Abstract

The aim of this study was to investigate the interaction of human FSH (10ng/ml) with T4 (20ng/mL) on survival, activation and growth of preantral follicles cultured in vitro for 28 days. Fragments of non-cultured and cultured ovarian tissue were processed for classic histology and transmission electron microscopy. The results showed a reduction in the survival rate in all the media tested (one to 28 days) when compared to the fresh control. However the treatment with T4/hFSH for seven days of culture maintained the rate similar to the control. The media tested by one and 28 days reduced the percentage of primordial follicles in all periods of culture. However, T4/hFSH on day one of culture remained similar to the fresh control. None of the media were able to keep the percentage of the developing follicles. It was observed that the follicular diameter in the medium with T4/hFSH remained similar to the fresh control. The ultrastructural analysis confirmed the integrity of follicles cultured for seven days in a medium supplemented with T4/hFSH. In conclusion, the medium with T4/hFSH is able to maintain the survival, promote the activation, and the ultrastructural integrity of caprine preantral follicles for until seven days.

Keywords: Activation; caprine; hFSH; preantral follicles; T4.

Downloads

Download data is not yet available.

Author Biographies

Sanely Lourenço da Costa, Universidade Federal de Viçosa/Doutora em Reprodução Animal

Departamento de Veterinária/Reprodução Animal

Eduardo Paulino da Costa, Universidade Federal de Viçosa/Professor

Departamento de Veterinária/ Reprodução Animal

Emílio César Martins Pereira, Universidade Estadual Paulista Júlio Mesquita Filho/ Doutorado

Departamento de Reprodução Animal e Radiologia Veterinária / Reprodução Animal

Wagner Gonzaga Gonçalves, Universidade Federal de Viçosa/Doutorado

Departamento de Biologia Geral / Biologia celular e estrutural

Talita Fernandes da Silva, Universidade Federal de Viçosa/Mestrado

Departamento de Veterinária / Reprodução Animal

Vanessa Lopes Dias Queiroz, Universidade Federal de Viçosa / Mestrado

Departamento de Veterinária / Reprodução Animal

Downloads

Published

2015-04-30

How to Cite

DA COSTA, S. L.; DA COSTA, E. P.; PEREIRA, E. C. M.; GONÇALVES, W. G.; DA SILVA, T. F.; QUEIROZ, V. L. D. HUMAN FOLLICLE STIMULATING HORMONE (hFSH) AND THYROXINE (T4) IN SURVIVAL MAINTENANCE AND IN VITRO GROWTH PROMOTION OF CAPRINE PREANTRAL FOLLICLES. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 16, n. 2, p. 298–312, 2015. DOI: 10.1590/cab.v16i2.31471. Disponível em: https://revistas.ufg.br/vet/article/view/31471. Acesso em: 21 nov. 2024.

Issue

Vol. 16 No. 2 (2015)

Section

Veterinary Medicine

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).