Sensibilidade do laminocultivo dermatobac® para o diagnóstico da endometrite fúngica em éguas

Eriky Akio de Oliveira Tongu; Carlos Peleschi Taborda; Shirlei Aparecida Vieira Marques; Gustavo Mendes Gomes; Letícia Patrão de Macedo Gomes; Kleber da Cunha Peixoto Jr.; André Maciel Crespilho

doi:10.1590/1089-6891v16i431444

SENSIBILITY OF MICROCULTIVATION ASSAY DERMATOBAC® FOR FUNGAL ENDOMETRITIS DIAGNOSIS IN MARES

Authors

Eriky Akio de Oliveira Tongu Universidade Federal de Uberlândia (UFU), Uberlândia, Minas Gerais, Brasil
Carlos Peleschi Taborda Universidade de São Paulo(USP), São Paulo, São Paulo, Brasil
Shirlei Aparecida Vieira Marques Universidade de São Paulo (USP), São Paulo, São Paulo, Brasil
Gustavo Mendes Gomes Universidade Severino Sombra, Vassouras, Rio de Janeiro, Brasil
Letícia Patrão de Macedo Gomes Universidade Severino Sombra, Vassouras, Rio de Janeiro, Brasil
Kleber da Cunha Peixoto Jr. Universidade de Santo Amaro (UNISA), São Paulo, São Paulo, Brasil
André Maciel Crespilho Universidade de Santo Amaro (UNISA), São Paulo, São Paulo, Brasil

DOI:

https://doi.org/10.1590/1089-6891v16i431444

Keywords:

Animal Reproduction

Abstract

Among different etiologic factors, fungal endometritis is a common cause of decreased mare’s fertility.Two experiments were conducted to evaluate the sensitivity of Dermatobac®, a microculture system originally developed for the isolation of fungi that concerns Human Medicine. In Experiment 1, standard(ATCC) strains of Candida (n=4) and Aspergyllus (n=1) were grown and visually analyzed daily for 7days. For Experiment 2, 34 mares were selected and subjected to material harvesting for completionof uterine cytology as well as seeding with Dermatobac®. After 24h of cultivation, the macroscopicobservation of all Candida and Aspergyllus strains (Experiment 1) was possible. In Experiment 2,five animals showed presence of yeast on cytologic slides (14.71%), results also confirmed sowingin Dermatobac® system (100% sensitivity). Five false positive cases were observed, characterizedby microbiological growth in Dermatobac® but not confirmed by endometrial cytology, determiningspecificity of 85%. However, the results did not compromise the efficiency of Dermatobac® systemgiven all contaminants presented differentiated morphology and higher growing time (P<0.05)when compared to the major etiological agents of equine fungal endometritis. The Dermatobac®system proved to be efficient for the macroscopic diagnosis of fungal endometritis in horses, showingconclusive information only after 24 hours of culture.

Keywords: Dermatobac®; diagnosis; endometritis; fungi; mare.

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Published

2015-10-30

How to Cite

TONGU, Eriky Akio de Oliveira; TABORDA, Carlos Peleschi; MARQUES, Shirlei Aparecida Vieira; GOMES, Gustavo Mendes; GOMES, Letícia Patrão de Macedo; PEIXOTO JR., Kleber da Cunha; CRESPILHO, André Maciel. SENSIBILITY OF MICROCULTIVATION ASSAY DERMATOBAC® FOR FUNGAL ENDOMETRITIS DIAGNOSIS IN MARES. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 16, n. 4, p. 598–607, 2015. DOI: 10.1590/1089-6891v16i431444. Disponível em: https://revistas.ufg.br/vet/article/view/31444. Acesso em: 14 dec. 2025.

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Issue

Vol. 16 No. 4 (2015)

Section

Veterinary Medicine

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