DESCRIPTION OF ULTRASTRUCTURAL DAMAGES IN FROZEN-THAWED CANINE SPERMATOZOA

Authors

  • Alexandre Rodrigues Silva Universidade Federal Rural do Semi Árido
  • José Domingues Fontenele-Neto Universidade Federal Rural do Semi-Árido
  • Rita de Cássia Soares Cardoso Universidade Federal do Piauí
  • Lúcia Daniel Machado da Silva Universidade Estadual do Ceará
  • Viviane Helena Chirinéa Universidade Estadual Paulista
  • Maria Denise Lopes Universidade Estadual Paulista

DOI:

https://doi.org/10.5216/cab.v10i2.3119

Keywords:

Reprodução Animal, Cão, Sêmen, Criopreservação, Ultra-estrutura

Abstract

In spite of the advances in transmission electronic microscopy (TEM), there are few studies presenting a systematic description of the canine spermatozoa and they are only focused on sperm cell heads. The goal of the present study was to describe ultrastructural appearance of canine fresh and frozen-thawed spermatozoa, focusing on damages induced by freezing-thawing in different sperm regions. Ten ejaculates from five proven stud dogs (two ejaculates/dog) were collected, evaluated, extended in Tris-egg yolk-glycerol, frozen and stored in liquid nitrogen, and thawed six weeks later.  Samples were evaluated for progressive motility, morphology, and for ultrastructural analysis by TEM. Concerning to TEM, the most striking differences between fresh and frozen-thawed samples were observed over the mid-piece since the fresh spermatozoa showed a well preserved mid-piece. However, the frozen-thawed spermatozoa mid-piece showed signs of damage such as mitochondrial vacuolization. In conclusion, freezing-thawing processes using a Tris-egg yolk extender induce ultrastructural damages in head and mid-piece of canine sperm, affecting the mitochondrial ultrastructure besides.

KEY WORDS: Cryopreservation, dog, semen, ultrastructure.

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Author Biographies

Alexandre Rodrigues Silva, Universidade Federal Rural do Semi Árido

Professor adjunto, Dr., Departamento de Ciências Animais, UFERSA, Mossoró-RN

José Domingues Fontenele-Neto, Universidade Federal Rural do Semi-Árido

Professor adjunto, Dr., Departamento de Ciências Animais, UFERSA, Mossoró, RN

Rita de Cássia Soares Cardoso, Universidade Federal do Piauí

Professora adjunta, Dr., Campus Profa. Cinobelina Elvas, UFPI, Bom Jesus, PI

Lúcia Daniel Machado da Silva, Universidade Estadual do Ceará

Professora adjunta, Dr., Laboratório de Reprodução de Carnívoros, UECE, Fortaleza, CE

Viviane Helena Chirinéa, Universidade Estadual Paulista

Doutoranda, Laboratório de Reprodução de Pequenos Animais e Silvestres, UNESP, Botucatu, SP

Maria Denise Lopes, Universidade Estadual Paulista

Professora adjunta, Livre Docente, Laboratório de Reprodução de Pequenos Animais e Silvestres, UNESP, Botucatu, SP

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Published

2009-07-03

How to Cite

SILVA, A. R.; FONTENELE-NETO, J. D.; CARDOSO, R. de C. S.; SILVA, L. D. M. da; CHIRINÉA, V. H.; LOPES, M. D. DESCRIPTION OF ULTRASTRUCTURAL DAMAGES IN FROZEN-THAWED CANINE SPERMATOZOA. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 10, n. 2, p. 595–601, 2009. DOI: 10.5216/cab.v10i2.3119. Disponível em: https://revistas.ufg.br/vet/article/view/3119. Acesso em: 22 nov. 2024.

Issue

Vol. 10 No. 2 (2009)

Section

Veterinary Medicine

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