CRYOTOLERANCE OF BOVINE OOCYTES AND EMBRYOS MATURATED WITH FOLLICULAR FLUID AND/OR ?-MERCAPTOETHANOL

Authors

  • Monalyza Cadori Gonçalves Universidade do Estado de Santa Catarina
  • Matheus Pedrotti Cesaro Universidade do Estado de Santa Catarina
  • Murilo Farias Rodrigues Universidade do Estado de Santa Catarina
  • Rui Félix Lopes Universidade Federal do Rio Grande do Sul
  • Joana Claudia Mezzalira Universidade do Estado de Santa Catarina
  • Fabricio Desconsi Mozzaquatro Universidade do Estado de Santa Catarina
  • Alceu Mezzalira Universidade do Estado de Santa Catarina

DOI:

https://doi.org/10.1590/cab.v16i2.27457

Keywords:

Animal Reproduction

Abstract

Cryotolerance of bovine oocytes and embryos maturated with addition of follicular fluid (LF) and ?-mercaptoethanol (BM) was evaluated. After vitrification, oocytes were maturated in: TCM-199 (control); BM (24h TCM-199+100µM BM); LF (6h in LF+18h TCM-199), and LF+BM (6h LF+18h TCM-199+100µM BM). There was not difference (p>0.05) in blastocysts rate in TCM (6.4%), BM (4.0%) and LF (3.4%) treatments. The hatching rates and cell density of hatched embryos did not differ (p>0.05) among treatments. In Experiment 2, hatched blastocysts (Bx) obtained in D7 or D8 were vitrified and evaluated according to its expanding and hatching rates. The expanding rate was similar (p>0.05), being observed a distinct pathway in hatching rate in D7 and D8 Bx. Higher hatching rate was observed in D7 Bx from control (TCM-54.2%) compared to BM (40.32%) and LF+BM (33.89%) treatments. The D8 Bx showed lower hatching rate in control (TCM-199) compared with D7 Bx. In BM, LF and LF+BM treatments, the hatching rate was similar for D7 or D8 embryos. Maturation with addition of LF and/or BM does not increase the oocyte or IVP embryo cryotolerance. Expanded blastocysts (D7) have higher cryotolerance and show a distinct pathway when added with LF or BM, in comparison with D8 embryos.

Keywords: additives; cryotolerance; IVP embryos; maturation; vitrification.

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Author Biographies

Monalyza Cadori Gonçalves, Universidade do Estado de Santa Catarina

Centro de Ciências Agroveterinárias

Matheus Pedrotti Cesaro, Universidade do Estado de Santa Catarina

Centro de Ciências Agroveterinárias

Murilo Farias Rodrigues, Universidade do Estado de Santa Catarina

Centro de Ciências Agroveterinárias

Joana Claudia Mezzalira, Universidade do Estado de Santa Catarina

Centro de Ciências Agroveterinárias

Fabricio Desconsi Mozzaquatro, Universidade do Estado de Santa Catarina

Centro de Ciências Agroveterinárias

Alceu Mezzalira, Universidade do Estado de Santa Catarina

Centro de Ciências Agroveterinárias

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Published

2015-04-30

How to Cite

GONÇALVES, M. C.; CESARO, M. P.; RODRIGUES, M. F.; LOPES, R. F.; MEZZALIRA, J. C.; MOZZAQUATRO, F. D.; MEZZALIRA, A. CRYOTOLERANCE OF BOVINE OOCYTES AND EMBRYOS MATURATED WITH FOLLICULAR FLUID AND/OR ?-MERCAPTOETHANOL. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 16, n. 2, p. 205–216, 2015. DOI: 10.1590/cab.v16i2.27457. Disponível em: https://revistas.ufg.br/vet/article/view/27457. Acesso em: 22 nov. 2024.

Issue

Vol. 16 No. 2 (2015)

Section

Animal Production

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