EVALUATING THE EFFECTIVENESS OF PERMEATING AND NON-PERMEATING CRYOPROTECTANTS IN FAST AND SLOW DEFROSTING OF CANINE SEMEN

Authors

  • Amanda Carla Acipreste UniRV-RIO VERDE -GO
  • Eduardo Paulino Costa Universidade Federal de Viçosa (UFV), MG
  • Fabrício Albani Oliveira Universidade Federal de Viçosa (UFV), MG
  • Sanely Lourenço Costa Universidade Federal de Viçosa (UFV), MG
  • Talita Fernandes Silva Universidade Federal de Viçosa (UFV), MG
  • Daniel Cortes Beretta UniRV-RIO VERDE -GO

DOI:

https://doi.org/10.5216/cab.v15i1.26124

Keywords:

Animal Reproduction

Abstract

The aim of this study was to evaluate the association between cryoprotectants little studied in Brazil such as dimethylformamide and trehalose amid thinner, using protocols of fast and slow defrosting. Three adult Labrador Retrievier male, healthy dogs, weekly submitted to one semen collection during five-weeks period, were used. The base diluent medium used in this study was tris-citrate added with 3% of dimethylformamide + 3% glycerol (D1), 3%  dimethylformamide and trehalose (D2) and 4% glycerol (D3). At defrosting, half of the semen samples from each diluent medium was defrosted by rapid method in water-bath at 75 °C for seven minutes, followed by a new immersion at 37 °C for 1 minute. The other half of the samples was defrosted by slow method, in water-bath at 37 °C for 1 minute. The semen was evaluated for sperm progressive motility and vigor, besides membrane integrity. For this, the semen samples were submitted to either hyposmotic and membrane integrity tests of the plasmatic membrane and acrosome  (fluorescence). The results indicated that the use of glycerol as cryoprotector in TRIS diluter provides greater efficacy in cryopreserving spermatozoa of the canine species, when compared to dimethylformamide associated with trehalose or glycerol.

KEYWORDS: cryopreservation; dimethylformamide; dogs; glycerol; trehalose.

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Author Biographies

Amanda Carla Acipreste, UniRV-RIO VERDE -GO

Médica Veterinária, Mestre, Professora Adjunta da Faculdade de Medicina Veterinária da Universidade de Rio Verde, UniRV-GO, Fazenda Fontes do Saber, Caixa Postal 104 - CEP: 75.901-970, Rio Verde - Goiás

Eduardo Paulino Costa, Universidade Federal de Viçosa (UFV), MG

Médico Veterinário, Mestre, Doutor, Departamento de Veterinária - Universidade Federal de Viçosa (UFV), Avenida Peter Henry Rolfs, s/n, 36571-000, Viçosa, Minas Gerais, Brasil (31) 3899-1457.

Fabrício Albani Oliveira, Universidade Federal de Viçosa (UFV), MG

Médico Veterinário, Doutorando. Departamento de Zootecnia, Universidade Federal de Viçosa (UFV), Avenida Peter Henry Rolfs, s/n, 36571-000, Viçosa, Minas Gerais, Brasil.

Sanely Lourenço Costa, Universidade Federal de Viçosa (UFV), MG

Médica Veterinária, Mestre. Departamento de Veterinária, Universidade Federal de Viçosa (UFV), Avenida Peter Henry Rolfs, s/n, 36571-000, Viçosa, Minas Gerais, Brasil. (31) 3899-1457. sanelylc@hotmail.com

Talita Fernandes Silva, Universidade Federal de Viçosa (UFV), MG

Médica Veterinária, Mestranda. Departamento de Veterinária, Universidade Federal de Viçosa (UFV), Avenida Peter Henry Rolfs, s/n, 36571-000, Viçosa, Minas Gerais, Brasil. (31) 3899-1457.

Daniel Cortes Beretta, UniRV-RIO VERDE -GO

Médico Veterinário, Mestre, Doutor, Professor de Patologia da Faculdade de Medicina Veterinária da Universidade de Rio Verde, UniRV-GO, Fazenda Fontes do Saber, Caixa Postal 104 - CEP: 75.901-970, Rio Verde - Goiás

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Published

2014-03-28

How to Cite

ACIPRESTE, A. C.; COSTA, E. P.; OLIVEIRA, F. A.; COSTA, S. L.; SILVA, T. F.; BERETTA, D. C. EVALUATING THE EFFECTIVENESS OF PERMEATING AND NON-PERMEATING CRYOPROTECTANTS IN FAST AND SLOW DEFROSTING OF CANINE SEMEN. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 15, n. 1, p. 107–114, 2014. DOI: 10.5216/cab.v15i1.26124. Disponível em: https://revistas.ufg.br/vet/article/view/26124. Acesso em: 16 jul. 2024.

Issue

Vol. 15 No. 1 (2014)

Section

Veterinary Medicine

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