QUALITY OF TAMBAQUI (Colossoma macropomum) SEMEN CRYOPRESERVED IN DIFFERENT CONCENTRATIONS OF EGG YOLK
DOI:
https://doi.org/10.1590/cab17225386Keywords:
Animal ReproductionAbstract
The aim of this study was to evaluate the effect of egg yolk (EY) addition on the sperm kinects after cryopreservation. We used twenty tambaqui males (n = 4 pools), which were induced homonally to spermiation with carp pituitary extract. Fourteen hours after induction, a seminal collection was performed. The semen from each pool was diluted with Ringer added 10% DMSO on the presence or absence of egg yolk (T1: no additional EY; T2: 5% EY; and T3: 10% EY). The treated semen was loaded in 0.5 mL straws, frozen in a nitrogen vapor vessel (dry shipper) (30 min / -153 °C), and then transferred to liquid nitrogen. Straws were thawed in a water bath at 37 °C / 30 seconds. The analyses of motility rate (%) and sperm curvilinear velocity (µm/s) were performed on computerized system (CASA). Data were expressed as mean ± standard deviation, and Tukey test was applied (P<0.05). There was a significant reduction in the percentage of motile spermatozoa and curvilinear velocity after the addition of egg yolk to the crioprotection solution, regardless of the concentration. Therefore, the addition of EY to Ringer + DMSO had negative effect on motility of tambaqui frozen sperm.
Keywords: extracellular cryoprotectant; freezing; reproduction.
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
