QUALITY OF TAMBAQUI (Colossoma macropomum) SEMEN CRYOPRESERVED IN DIFFERENT CONCENTRATIONS OF EGG YOLK

Authors

  • João Paulo Silva Pinheiro UECE/Programa de Pós-Graduação em Ciências Veterinárias
  • Liliane Veras Leite-Castro UECE/Programa de Pós-Graduação em Ciências Veterinárias
  • Fátima de Cássia Evangelista de Oliveira UECE/Programa de Pós-Graduação em Ciências Veterinárias
  • Francisco Renan Aragão Linhares UECE/Programa de Pós-Graduação em Ciências Veterinárias
  • Júlia Trugílio Lopes UECE/Programa de Pós-Graduação em Ciências Veterinárias
  • Carminda Sandra Brito Salmito-Vanderley UECE / Programa de Pós-Graduação em Ciências Veterinárias

DOI:

https://doi.org/10.1590/cab17225386

Keywords:

Animal Reproduction

Abstract

The aim of this study was to evaluate the effect of egg yolk (EY) addition on the sperm kinects after cryopreservation. We used twenty tambaqui males (n = 4 pools), which were induced homonally to spermiation with carp pituitary extract. Fourteen hours after induction, a seminal collection was performed. The semen from each pool was diluted with Ringer added 10% DMSO on the presence or absence of egg yolk (T1: no additional EY; T2: 5% EY; and T3: 10% EY). The treated semen was loaded in 0.5 mL straws, frozen in a nitrogen vapor vessel (dry shipper) (30 min / -153 °C), and then transferred to liquid nitrogen. Straws were thawed in a water bath at 37 °C / 30 seconds. The analyses of motility rate (%) and sperm curvilinear velocity (µm/s) were performed on computerized system (CASA). Data were expressed as mean ± standard deviation, and Tukey test was applied (P<0.05). There was a significant reduction in the percentage of motile spermatozoa and curvilinear velocity after the addition of egg yolk to the crioprotection solution, regardless of the concentration. Therefore, the addition of EY to Ringer + DMSO had negative effect on motility of tambaqui frozen sperm.
Keywords: extracellular cryoprotectant; freezing; reproduction.

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Author Biography

Carminda Sandra Brito Salmito-Vanderley, UECE / Programa de Pós-Graduação em Ciências Veterinárias

Professora, orientadora do Programa de Pós-Graduação em Ciências Veterinárias - PPGCV/UECE

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Published

2016-04-29

How to Cite

PINHEIRO, J. P. S.; LEITE-CASTRO, L. V.; OLIVEIRA, F. de C. E. de; LINHARES, F. R. A.; LOPES, J. T.; SALMITO-VANDERLEY, C. S. B. QUALITY OF TAMBAQUI (Colossoma macropomum) SEMEN CRYOPRESERVED IN DIFFERENT CONCENTRATIONS OF EGG YOLK. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 17, n. 2, p. 267–273, 2016. DOI: 10.1590/cab17225386. Disponível em: https://revistas.ufg.br/vet/article/view/25386. Acesso em: 18 may. 2024.

Issue

Vol. 17 No. 2 (2016)

Section

Veterinary Medicine

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