Eficiência dos diluidores tris e botu-crio® sobre os parâmetros seminais de garanhões das raças quarto de milha e mangalarga marchador

Deyse Naira Mascarenhas Costa; Daniel Anderson Martiliano Silva; Yatta Linhares Boakari; Siluana Benvindo Ferreira; Marlon Araújo Castelo Branco; José Adalmir Torres de Souza

doi:10.1590/1809-6891v15i325327

EFFICIENCY OF TRIS EXTENDERS AND BOTU-CRIO ® ON SEMINAL PARAMETERS IN STALLION BREEDS QUARTER HORSE AND MANGALARGA MARCHADOR

Authors

Deyse Naira Mascarenhas Costa Universidade Federal do Piauí (UFPI), Teresina, Piauí, Brasil
Daniel Anderson Martiliano Silva Universidade Federal do Piauí (UFPI), Teresina, Piauí, Brasil
Yatta Linhares Boakari Universidade Estadual Paulista (UNESP), Botucatu, São Paulo, Brasil
Siluana Benvindo Ferreira Universidade Federal do Piauí (UFPI), Teresina, Piauí, Brasil
Marlon Araújo Castelo Branco Universidade Federal do Piauí (UFPI), Teresina, Piauí, Brasil
José Adalmir Torres de Souza Universidade Federal do Piauí (UFPI), Teresina, Piauí, Brasil

DOI:

https://doi.org/10.1590/1809-6891v15i325327

Keywords:

Animal Sanity

Abstract

The limiting factor in the cryopreservation process of equine semen is related to the species, as they present a great variability in the ejaculate’s characteristics after thawing. The aim of this study was to evaluate sperm viability after thawing, from Quarter Horse and Mangalarga Marchador stallions using two extenders (Botu-crio and Tris) in cryopreservation. To this end, we analyzed the physical characteristics of fresh semen, the sperm membrane functionality by the hypoosmotic swelling test (HOST), total motility and vigor by the Thermoresistance Test (TRT), progressive motility by a computerized system CASA (Computer-Assisted Semen Anlyses) and acrosomal membrane integrity and functionality of the mitochondria by epifluorescence microscopy. After thawing, the extender Botu-crio® better preserved motility, vigor and integrity of the plasma membrane. There was no significant difference between breeds for the thermoresistance test after twaing. Quarter Horse showed higher percentage of bigger defects in the sperm pathology analysis.

KEYWORDS: cryopreservation, equine, extender, semen.

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Author Biography

Marlon Araújo Castelo Branco, Universidade Federal do Piauí (UFPI), Teresina, Piauí, Brasil

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Published

2014-09-27

How to Cite

COSTA, Deyse Naira Mascarenhas; SILVA, Daniel Anderson Martiliano; BOAKARI, Yatta Linhares; FERREIRA, Siluana Benvindo; BRANCO, Marlon Araújo Castelo; SOUZA, José Adalmir Torres de. EFFICIENCY OF TRIS EXTENDERS AND BOTU-CRIO ® ON SEMINAL PARAMETERS IN STALLION BREEDS QUARTER HORSE AND MANGALARGA MARCHADOR. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 15, n. 3, p. 322–329, 2014. DOI: 10.1590/1809-6891v15i325327. Disponível em: https://revistas.ufg.br/vet/article/view/25327. Acesso em: 6 dec. 2025.

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Issue

Vol. 15 No. 3 (2014)

Section

Veterinary Medicine

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