EFICIÊNCIA DOS DILUIDORES TRIS E BOTU-CRIO® SOBRE OS PARÂMETROS SEMINAIS DE GARANHÕES DAS RAÇAS QUARTO DE MILHA E MANGALARGA MARCHADOR

Deyse Naira Mascarenhas Costa; Daniel Anderson Martiliano Silva; Yatta Linhares Boakari; Siluana Benvindo Ferreira; Marlon Araújo Castelo Branco; José Adalmir Torres de Souza

doi:10.5216/cab.v15i3.25327

EFFICIENCY OF TRIS EXTENDERS AND BOTU-CRIO ® ON SEMINAL PARAMETERS IN STALLION BREEDS QUARTER HORSE AND MANGALARGA MARCHADOR

Authors

Deyse Naira Mascarenhas Costa Universidade Federal do Piauí-UFPI
Daniel Anderson Martiliano Silva Universidade Federal do Piauí-UFPI
Yatta Linhares Boakari Universidade Estadual Júlio de Mesquita Filho- Botucatu-SP
Siluana Benvindo Ferreira Universidade Federal do Piauí-UFPI
Marlon Araújo Castelo Branco Universidade Federal do Piauí- UFPI
José Adalmir Torres de Souza Universidade Federal do Piauí-UFPI

DOI:

https://doi.org/10.5216/cab.v15i3.25327

Keywords:

Animal Sanity

Abstract

The limiting factor in the cryopreservation process of equine semen is related to the species, as they present a great variability in the ejaculate’s characteristics after thawing. The aim of this study was to evaluate sperm viability after thawing, from Quarter Horse and Mangalarga Marchador stallions using two extenders (Botu-crio and Tris) in cryopreservation. To this end, we analyzed the physical characteristics of fresh semen, the sperm membrane functionality by the hypoosmotic swelling test (HOST), total motility and vigor by the Thermoresistance Test (TRT), progressive motility by a computerized system CASA (Computer-Assisted Semen Anlyses) and acrosomal membrane integrity and functionality of the mitochondria by epifluorescence microscopy. After thawing, the extender Botu-crio® better preserved motility, vigor and integrity of the plasma membrane. There was no significant difference between breeds for the thermoresistance test after twaing. Quarter Horse showed higher percentage of bigger defects in the sperm pathology analysis.

KEYWORDS: cryopreservation, equine, extender, semen.

Downloads

Download data is not yet available.

Author Biographies

Deyse Naira Mascarenhas Costa, Universidade Federal do Piauí-UFPI

Doutoranda do Programa de Pós-graduação em Ciência Animal-UFPI. Área: Sanidade e Reprodução Animal.

Daniel Anderson Martiliano Silva, Universidade Federal do Piauí-UFPI

Graduado em Medicina Veterinária.

Membro do Laboratório de Biotecnologia da Reprodução Animal- UFPI

Yatta Linhares Boakari, Universidade Estadual Júlio de Mesquita Filho- Botucatu-SP

Mestranda na Universidade Estadual Júlio de Mesquita Filho- Botucatu-SP

Siluana Benvindo Ferreira, Universidade Federal do Piauí-UFPI

Doutoranda em Ciência Animal no Programa de Pós-graduação em Ciência Animal- Área de Sanidade e Reprodução Animal- UFPI

Marlon Araújo Castelo Branco, Universidade Federal do Piauí- UFPI

Membro do Laboratório de Biotecnologia da Reprodução Animal- UFPI.

Doutorando em Biotecnologia - RENORBIO

José Adalmir Torres de Souza, Universidade Federal do Piauí-UFPI

Profº Drº de Fisopatologia da Reprodução do Macho e Biotecnologia da Reprodução Animal.

Coordenador do Programa de Pós-graduação em Ciência Animal- UFPI

Coordenador do Laboratório de Biotecnologia da Reprodução Animal- UFPI.

Downloads

Published

2014-09-27

How to Cite

COSTA, D. N. M.; SILVA, D. A. M.; BOAKARI, Y. L.; FERREIRA, S. B.; CASTELO BRANCO, M. A.; DE SOUZA, J. A. T. EFFICIENCY OF TRIS EXTENDERS AND BOTU-CRIO ® ON SEMINAL PARAMETERS IN STALLION BREEDS QUARTER HORSE AND MANGALARGA MARCHADOR. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 15, n. 3, p. 322–329, 2014. DOI: 10.5216/cab.v15i3.25327. Disponível em: https://revistas.ufg.br/vet/article/view/25327. Acesso em: 16 jan. 2025.

Download Citation

Issue

Vol. 15 No. 3 (2014)

Section

Veterinary Medicine

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).