EFFICIENCY OF TRIS EXTENDERS AND BOTU-CRIO ® ON SEMINAL PARAMETERS IN STALLION BREEDS QUARTER HORSE AND MANGALARGA MARCHADOR

Authors

  • Deyse Naira Mascarenhas Costa Universidade Federal do Piauí-UFPI
  • Daniel Anderson Martiliano Silva Universidade Federal do Piauí-UFPI
  • Yatta Linhares Boakari Universidade Estadual Júlio de Mesquita Filho- Botucatu-SP
  • Siluana Benvindo Ferreira Universidade Federal do Piauí-UFPI
  • Marlon Araújo Castelo Branco Universidade Federal do Piauí- UFPI
  • José Adalmir Torres de Souza Universidade Federal do Piauí-UFPI

DOI:

https://doi.org/10.5216/cab.v15i3.25327

Keywords:

Animal Sanity

Abstract

The limiting factor in the cryopreservation process of equine semen is related to the species, as they present a great variability in the ejaculate’s characteristics after thawing. The aim of this study was to evaluate sperm viability after thawing, from Quarter Horse and Mangalarga Marchador stallions using two extenders (Botu-crio and Tris) in cryopreservation. To this end, we analyzed the physical characteristics of fresh semen, the sperm membrane functionality by the hypoosmotic swelling test (HOST), total motility and vigor by the Thermoresistance Test (TRT), progressive motility by a computerized system CASA (Computer-Assisted Semen Anlyses) and acrosomal membrane integrity and functionality of the mitochondria by epifluorescence microscopy. After thawing, the extender Botu-crio® better preserved motility, vigor and integrity of the plasma membrane. There was no significant difference between breeds for the thermoresistance test after twaing. Quarter Horse showed higher percentage of bigger defects in the sperm pathology analysis.

KEYWORDS: cryopreservation, equine, extender, semen.

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Author Biographies

Deyse Naira Mascarenhas Costa, Universidade Federal do Piauí-UFPI

Doutoranda do Programa de Pós-graduação em Ciência Animal-UFPI. Área: Sanidade e Reprodução Animal.

Daniel Anderson Martiliano Silva, Universidade Federal do Piauí-UFPI

Graduado em Medicina Veterinária.

Membro do Laboratório de Biotecnologia da Reprodução Animal- UFPI

Yatta Linhares Boakari, Universidade Estadual Júlio de Mesquita Filho- Botucatu-SP

Mestranda na Universidade Estadual Júlio de Mesquita Filho- Botucatu-SP

Siluana Benvindo Ferreira, Universidade Federal do Piauí-UFPI

Doutoranda em Ciência Animal no Programa de Pós-graduação em Ciência Animal- Área de Sanidade e Reprodução Animal- UFPI

Marlon Araújo Castelo Branco, Universidade Federal do Piauí- UFPI

Membro do Laboratório de Biotecnologia da Reprodução Animal- UFPI.

Doutorando em Biotecnologia - RENORBIO

 

José Adalmir Torres de Souza, Universidade Federal do Piauí-UFPI

Profº Drº de Fisopatologia da Reprodução do Macho e Biotecnologia da Reprodução Animal.

Coordenador do Programa de Pós-graduação em Ciência Animal- UFPI

Coordenador do Laboratório de Biotecnologia da Reprodução Animal- UFPI.

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Published

2014-09-27

How to Cite

COSTA, D. N. M.; SILVA, D. A. M.; BOAKARI, Y. L.; FERREIRA, S. B.; CASTELO BRANCO, M. A.; DE SOUZA, J. A. T. EFFICIENCY OF TRIS EXTENDERS AND BOTU-CRIO ® ON SEMINAL PARAMETERS IN STALLION BREEDS QUARTER HORSE AND MANGALARGA MARCHADOR. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 15, n. 3, p. 322–329, 2014. DOI: 10.5216/cab.v15i3.25327. Disponível em: https://revistas.ufg.br/vet/article/view/25327. Acesso em: 12 may. 2024.

Issue

Vol. 15 No. 3 (2014)

Section

Veterinary Medicine

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