CENTRIFUGATION OF OVINE SPERM IN CONTINUOUS DENSITY GRADIENT: EFFECT ON PREGNANCY RATE AND SEX RATIO

Authors

  • Max Vitória Resende Setor de Reprodução Animal - EMVZ - UFBA
  • Carmo Emanuel Almeida Biscarde Universidade Federal do Recôncavo da Bahia
  • Laura Emília Panelli Martins Setor de Reprodução Animal - EMVZ - UFBA
  • Cláudia Kazumi Kiya Setor de Reprodução Animal - EMVZ - UFBA
  • Vera Fernanda Martins Hossepian de Lima Departamento de Reprodução Animal - FCAV - UNESP
  • Alberto Lopes Gusmão Setor de Reprodução Animal - EMVZ - UFBA

DOI:

https://doi.org/10.1590/cab.v16i1.24607

Keywords:

Animal Reproduction

Abstract

The objective of this study was to evaluate the efficiency of continuous density gradients of Percoll and OptiPrep for separation of X-bearing sperm, and assess the influence on sperm viability, pregnancy rate and sex ratio. With this purpose 100 million spermatozoa were deposited into each tube containing density gradient, which were previously prepared in polystyrene tubes, 24 hours before centrifugation and stored at 4 °C. The tubes were centrifuged at 500xg for 15 minutes at 22 °C, and then the supernatants aspirated and sperm recovered from the bottom of the tubes. A sample was evaluated before and after centrifugation to verify motility and sperm vigor. The sperm were used for fixed time artificial insemination. The results indicated that centrifugation did not affect sperm viability and pregnancy rate, however there was no deviation of sex ratio for females. More studies are needed of adapting the centrifugation technique to sheep and thus obtain the deviation of sex ration as in bovine.

Keywords: ram; semen; sexing; sperm viability.

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Author Biographies

Max Vitória Resende, Setor de Reprodução Animal - EMVZ - UFBA

Carmo Emanuel Almeida Biscarde, Universidade Federal do Recôncavo da Bahia

Laura Emília Panelli Martins, Setor de Reprodução Animal - EMVZ - UFBA

Cláudia Kazumi Kiya, Setor de Reprodução Animal - EMVZ - UFBA

Vera Fernanda Martins Hossepian de Lima, Departamento de Reprodução Animal - FCAV - UNESP

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Published

2015-01-31

How to Cite

RESENDE, M. V.; BISCARDE, C. E. A.; MARTINS, L. E. P.; KIYA, C. K.; HOSSEPIAN DE LIMA, V. F. M.; GUSMÃO, A. L. CENTRIFUGATION OF OVINE SPERM IN CONTINUOUS DENSITY GRADIENT: EFFECT ON PREGNANCY RATE AND SEX RATIO. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 16, n. 1, p. 125–132, 2015. DOI: 10.1590/cab.v16i1.24607. Disponível em: https://revistas.ufg.br/vet/article/view/24607. Acesso em: 17 jul. 2024.

Issue

Vol. 16 No. 1 (2015)

Section

Veterinary Medicine

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