EFFECT OF DIFFERENT THAWING PROTOCOLS ON CANINE SEMEN CRYOPRESERVED IN A POWDERED COCONUT WATER EXTENDER (ACP-106®)

Authors

  • Victor Leão Hitzschky Madeira Universidade Estadual do Ceará
  • Cynthia Levi Baratta Monteiro Universidade Estadual do Ceará
  • Claudia da Cunha Barbosa Universidade Estadual do Ceará
  • Ricardo Parente Jucá Universidade Estadual do Ceará
  • Ângela Cristina de Oliveira Universidade Estadual do Ceará
  • Daniel Couto Uchoa Universidade Estadual do Ceará
  • Lúcia Daniel Machado da Silva Universidade Estadual do Ceará

DOI:

https://doi.org/10.5216/cab.v11i4.2449

Keywords:

Animal Reproduction

Abstract

It is known that temperature and thawing velocity can affect sperm quality during freeze-thawing procedures. Thus, the aim of this research was to compare different thawing protocols for canine semen extended in ACP-106® extender and cryopreserved. Ten ejaculates were collected from eight dogs and sperm rich fraction was evaluated regarding volume, concentration, motility, vigor, pH, morphology, acrosomal integrity and hyposmotic swelling test. Then, semen was extended in ACP-106® with 20% egg yolk and 6% glycerol, and frozen in 0.25 mL straws. After one week, one straw was thawed at 37°C/60s, another one at 55°C/5s and a third one at 75°C/8s, and the seminal characteristics were reevaluated. Similar results were observed for all the groups, except for sperm motility which was better preserved at 55°C/5s for up 30min than at 37°C/60s or 75°C/8s. From these results, it is concluded that canine semen extended in ACP -106® is better thawed at 55°C/5s.

KEY WORDS: ACP-106®, cryopreservation, dog, semen, thawing.

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Author Biographies

Victor Leão Hitzschky Madeira, Universidade Estadual do Ceará

Médico Veterinário, mestrando do Programa de Pós-Graduação em Ciências Veterinárias da Universidade Estadual do Ceará.

Cynthia Levi Baratta Monteiro, Universidade Estadual do Ceará

Médica Veterinária, mestranda do Programa de Pós-Graduação em Ciências Veterinárias da Universidade Estadual do Ceará.

Claudia da Cunha Barbosa, Universidade Estadual do Ceará

Médica Veterinária, mestranda do Programa de Pós-Graduação em Ciências Veterinárias da Universidade Estadual do Ceará.

Ricardo Parente Jucá, Universidade Estadual do Ceará

Acadêmico da Medicina Veterinária da Universidade Estadual do Ceará.

Ângela Cristina de Oliveira, Universidade Estadual do Ceará

Acadêmica da Medicina Veterinária da Universidade Estadual do Ceará.

Daniel Couto Uchoa, Universidade Estadual do Ceará

Médico Veterinário, doutorando do Programa de Pós-Graduação Rede Nordeste de Biotecnologia.

Lúcia Daniel Machado da Silva, Universidade Estadual do Ceará

Médica Veterinária, professor adjunto da Faculdade de Veterinária da Universidade Estadual do Ceará.

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Published

2010-12-21

How to Cite

MADEIRA, V. L. H.; MONTEIRO, C. L. B.; BARBOSA, C. da C.; JUCÁ, R. P.; OLIVEIRA, Ângela C. de; UCHOA, D. C.; SILVA, L. D. M. da. EFFECT OF DIFFERENT THAWING PROTOCOLS ON CANINE SEMEN CRYOPRESERVED IN A POWDERED COCONUT WATER EXTENDER (ACP-106®). Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 11, n. 4, p. 845–852, 2010. DOI: 10.5216/cab.v11i4.2449. Disponível em: https://revistas.ufg.br/vet/article/view/2449. Acesso em: 17 jul. 2024.

Issue

Vol. 11 No. 4 (2010)

Section

Veterinary Medicine

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