CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION

Authors

  • Julio Cesar Oliveira Dias Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brasil
  • Madriano Christilis da Rocha Santos Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brasil
  • Jurandy Mauro Penitente Filho Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brasil
  • Gisele Dias Oliveira Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brasil
  • Vivian Rachel Araujo Mendes Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brasil
  • Antonio Bento Mancio Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brasil

DOI:

https://doi.org/10.1590/cab.v16i2.24240

Keywords:

Animal Reproduction

Abstract

Cryopreservation of semen is of great importance for various reproductive biotechnologies such as artificial insemination (IA), In Vitro Embryo Production (PIV) and Intracytoplasmic Sperm Injection (ICSI). The objective of this work was to evaluate the stability and persistence of motility and strength of sperm, as well as changes in the plasma membrane after the addition of Ringer Lactate, sodium citrate 2.92% or TRIS solution in thawed goat semen. Semen was collected from two Alpine Brown goats, and standard procedures for cryopreservation and seminal analysis were performed. After thawing the semen, the extenders Ringer Lactate, sodium citrate 2.92% and TRIS solution were added and Thermoresistance (TTR), Supravital and Morphology Tests were carried out. In TTR, only the group received TRIS solution presented motility and strength for a longer period (90 minutes; P< 0,05). No influence of the addition of the solutions was found in the morphological analysis, as well as in the Supravital Test, which showed values ??close to those found for motility (P> 0.05). We concluded that the addition of the solutions does not allow a large persistence of motility and strength of thawed sperm, but TRIS solution could be used for expansion of seminal doses used in vitro reproductive biotechnology.

Keywords: extender, goat, semen, spermatozoa.

Downloads

Download data is not yet available.

Author Biography

Julio Cesar Oliveira Dias, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brasil

Medico Veterinária, atuante na área de Fisiologia e Reprodução Animal de ruminantes (bovino, caprino e ovino)

Downloads

Published

2015-04-30

How to Cite

DIAS, J. C. O.; SANTOS, M. C. da R.; PENITENTE FILHO, J. M.; OLIVEIRA, G. D.; MENDES, V. R. A.; MANCIO, A. B. CHARACTERISTICS OF THAWED GOAT SEMEN AFTER ADDITION OF RINGER LACTATE, SODIUM CITRATE AND TRIS SOLUTION. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 16, n. 2, p. 243–250, 2015. DOI: 10.1590/cab.v16i2.24240. Disponível em: https://revistas.ufg.br/vet/article/view/24240. Acesso em: 17 may. 2024.

Issue

Vol. 16 No. 2 (2015)

Section

Veterinary Medicine

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).