CRYOPRESERVATION CANINE SEMEN USING A POWDERED COCONUT WATER EXTENDER (APC-106): EFFECT OF GLYCEROL TEMPERATURE ADDITION (27OC AND 4OC)

Authors

  • Claudia da Cunha Barbosa Universidade Estadual do Ceará
  • Victor Leão Hitzschky Madeira Universidade Estadual do Ceará
  • Ricardo Parente Jucá Universidade Estadual do Ceará
  • Ângela Cristina de Oliveira Universidade Estadual do Ceará
  • Daniel Couto Uchoa Universidade Estadual do Ceará
  • Lúcia Daniel Machado da Silva Universidade Estadual do Ceará

DOI:

https://doi.org/10.5216/cab.v10i4.2356

Keywords:

Animal production

Abstract

The aim of the present study was to compare the effect of glycerol addition at two temperatures on canine semen extended and frozen with powdered coconut water extender (ACP-106®). Twelve ejaculates were collected, the sperm-rich fraction was evaluated and further divided into two aliquots. The first aliquot was extended in ACP-106® with 5% egg yolk and glycerol at 27ºC (G27) and the second one was also extended in ACP-106® with 5% egg yolk, with glycerol addition at 4ºC (G4). Samples were frozen, thawed and submitted to evaluations of morphology, acrossomal integrity, hypo-osmotic swelling and alive spermatozoa. No differences were observed between groups after thawing regarding all seminal parameters. In computerized analyzes, it was also not evidenced differences between groups for total and progressive motility, percentual of spermatozoa with fast and medium velocity, and average path velocity (VAP) of spermatozoa with fast and medium velocity, being observed in G4 and G27, respectively, a total motility of 24.45 ± 3.87% and 31.65 ± 3.93% with the VAP of the fast spermatozoa of 91.24 ± 7.74µm/s and 106.25 ± 3.94µm/s. In conclusion, the glycerol temperature addition does not influence the quality of post-thawed canine semen diluted in ACP-106®.
KEY WORDS: ACP-106®, cryopreservation, dog, glycerol, semen. 

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Author Biographies

Claudia da Cunha Barbosa, Universidade Estadual do Ceará

Mestranda do Programa de Pós-Graduação em Ciências Veterinárias da UECE.

Victor Leão Hitzschky Madeira, Universidade Estadual do Ceará

Mestrando do Programa de Pós-Graduação em Ciências Veterinárias da UECE.

Ricardo Parente Jucá, Universidade Estadual do Ceará

Acadêmico de Medicina Veterinária da UECE.

Ângela Cristina de Oliveira, Universidade Estadual do Ceará

Acadêmica de Medicina Veterinária da UECE.

Daniel Couto Uchoa, Universidade Estadual do Ceará

Doutorando da Rede Nordeste de Biotecnologia.

Lúcia Daniel Machado da Silva, Universidade Estadual do Ceará

Departamento de Medicina Veterinária.

Área: Reprodução de Carnívoros

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Published

2009-12-17

How to Cite

BARBOSA, C. da C.; MADEIRA, V. L. H.; JUCÁ, R. P.; OLIVEIRA, Ângela C. de; UCHOA, D. C.; SILVA, L. D. M. da. CRYOPRESERVATION CANINE SEMEN USING A POWDERED COCONUT WATER EXTENDER (APC-106): EFFECT OF GLYCEROL TEMPERATURE ADDITION (27OC AND 4OC). Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 10, n. 4, p. 1207–1215, 2009. DOI: 10.5216/cab.v10i4.2356. Disponível em: https://revistas.ufg.br/vet/article/view/2356. Acesso em: 17 jul. 2024.

Issue

Vol. 10 No. 4 (2009)

Section

Veterinary Medicine

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