EFFECT OF WATER EXTRACT FROM BROWN PROPOLIS ON PRODUCTION OF IFN-? AFTER IMMUNIZATION AGAINST CANINE PARVOVIRUS (CPV) AND CANINE CORONAVIRUS (CCoV)

Authors

  • Maureen Hoch Vieira Fernandes Universidade Federal de Pelotas
  • Lilian das Neves Ferreira
  • Gilberto D'Avila Vargas UFPel
  • Geferson Fischer UFPel
  • Silvia de Oliveira Hübner UFPel

DOI:

https://doi.org/10.1590/cab.v16i2.23458

Keywords:

apiculture

Abstract

The purpose of this study was to evaluate the imunnostimulatory adjuvant capacity of water extract from brown propolis (WEBP) when added to a vaccine against canine parvovirus (CPV) and canine coronavirus (CCoV), regarding the production of IFN-?. Mice were vaccinated with CPV and CCoV (3.0 x 106 TCID50) with or without 400 µg/dose of WEBP. Thirty days after the third dose, splenocytes were cultured to measure the expression levels of IFN-? mRNA in the immunized animals. Increased levels of IFN-? mRNA expression for CCoV were evidenced by RT-PCR, in the splenocytes of mice inoculated with the vaccine containing 400 ?g/dose of WEBP, demonstrating the ability of propolis to stimulate cellular immune responses against the antigens of this virus. In contrast, the levels of IFN-? to CPV were not influenced by the presence of WEBP.

Keywords: adjuvant; canine coronavirus; canine parvovirus; propolis; vaccine.

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Author Biographies

Maureen Hoch Vieira Fernandes, Universidade Federal de Pelotas

Departamento de Veterinária Preventiva, Laboratório de Virologia e Imunologia

Lilian das Neves Ferreira

Médico Veterinário autônomo

Gilberto D'Avila Vargas, UFPel

Departamento de Veterinária Preventiva, Laboratório de Virologia e Imunologia

Geferson Fischer, UFPel

Departamento de Veterinária Preventiva, Laboratório de Virologia e Imunologia

Silvia de Oliveira Hübner, UFPel

Departamento de Veterinária Preventiva, Laboratório de Virologia e Imunologia

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Published

2015-04-30

How to Cite

FERNANDES, M. H. V.; FERREIRA, L. das N.; VARGAS, G. D.; FISCHER, G.; HÜBNER, S. de O. EFFECT OF WATER EXTRACT FROM BROWN PROPOLIS ON PRODUCTION OF IFN-? AFTER IMMUNIZATION AGAINST CANINE PARVOVIRUS (CPV) AND CANINE CORONAVIRUS (CCoV). Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 16, n. 2, p. 235–242, 2015. DOI: 10.1590/cab.v16i2.23458. Disponível em: https://revistas.ufg.br/vet/article/view/23458. Acesso em: 22 nov. 2024.

Issue

Vol. 16 No. 2 (2015)

Section

Veterinary Medicine

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