Efeito do extrato aquoso de própolis marrom sobre a produção de ifn-? após imunização contra parvovírus canino (CPV) e coronavírus canino (CCoV)

Maureen Hoch Vieira Fernandes; Lilian das Neves Ferreira; Gilberto D'Avila Vargas; Geferson Fischer; Silvia de Oliveira Hübner

doi:10.1590/1089-6891v16i223458

EFFECT OF WATER EXTRACT FROM BROWN PROPOLIS ON PRODUCTION OF IFN-? AFTER IMMUNIZATION AGAINST CANINE PARVOVIRUS (CPV) AND CANINE CORONAVIRUS (CCoV)

Authors

Maureen Hoch Vieira Fernandes Universidade Federal de Pelotas (UFPEL), Pelotas, Rio Grande do Sul, Brasil
Lilian das Neves Ferreira Universidade Federal de Pelotas (UFPEL), Pelotas, Rio Grande do Sul, Brasil
Gilberto D'Avila Vargas Universidade Federal de Pelotas (UFPEL), Pelotas, Rio Grande do Sul, Brasil
Geferson Fischer Universidade Federal de Pelotas (UFPEL), Pelotas, Rio Grande do Sul, Brasil
Silvia de Oliveira Hübner Universidade Federal de Pelotas (UFPEL), Pelotas, Rio Grande do Sul, Brasil

DOI:

https://doi.org/10.1590/1089-6891v16i223458

Keywords:

apiculture

Abstract

The purpose of this study was to evaluate the imunnostimulatory adjuvant capacity of water extract from brown propolis (WEBP) when added to a vaccine against canine parvovirus (CPV) and canine coronavirus (CCoV), regarding the production of IFN-?. Mice were vaccinated with CPV and CCoV (3.0 x 10⁶ TCID₅₀) with or without 400 µg/dose of WEBP. Thirty days after the third dose, splenocytes were cultured to measure the expression levels of IFN-? mRNA in the immunized animals. Increased levels of IFN-? mRNA expression for CCoV were evidenced by RT-PCR, in the splenocytes of mice inoculated with the vaccine containing 400 ?g/dose of WEBP, demonstrating the ability of propolis to stimulate cellular immune responses against the antigens of this virus. In contrast, the levels of IFN-? to CPV were not influenced by the presence of WEBP.

Keywords: adjuvant; canine coronavirus; canine parvovirus; propolis; vaccine.

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Published

2015-04-30

How to Cite

FERNANDES, Maureen Hoch Vieira; FERREIRA, Lilian das Neves; VARGAS, Gilberto D’Avila; FISCHER, Geferson; HÜBNER, Silvia de Oliveira. EFFECT OF WATER EXTRACT FROM BROWN PROPOLIS ON PRODUCTION OF IFN-? AFTER IMMUNIZATION AGAINST CANINE PARVOVIRUS (CPV) AND CANINE CORONAVIRUS (CCoV). Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 16, n. 2, p. 235–242, 2015. DOI: 10.1590/1089-6891v16i223458. Disponível em: https://revistas.ufg.br/vet/article/view/23458. Acesso em: 6 dec. 2025.

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Issue

Vol. 16 No. 2 (2015)

Section

Veterinary Medicine

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