PRODUCTION OF HYBRIDOMAS SECRETING ANTI-Neospora caninum ANTIBODIES FOR USE IN IMMUNODIAGNOSTIC

Authors

  • Bruna Alves Devens Centro Universitário do Espírito Santo-UNESC
  • Marlene Isabel Vargas Viloria Professora Doutora Universidade Federal de Viçosa
  • Carlos Henryque de Souza e Silva Pós doutorando pela Universidade Federal de Ouro Preto
  • Joaquin Hernán Patarroyo Salcedo Professor Doutor Universidade Federal de Viçosa

DOI:

https://doi.org/10.5216/cab.v15i2.23017

Keywords:

Animal sanity

Abstract

The Neospora caninum is a protozoan Apicomplexa with greater involvement in abortions worldwide. The economic losses determined by neosporosis also include abortions besides the early disposal of cows, costs for replacing animals in the herd, drop in milk production as well as milk in fat production. The immunological diagnosis involves purchasing costly diagnostic kits on the market. Therefore, the aim of this study was the production of hybridomas secreting polyclonal antibodies with affinity to Neospora caninum (Nc-1 strain) for immunodiagnostic use. For antibodies production, we used sonicated protozoa from Vero cells in culture, purified by filtration. These tachyzoites were employed for immunization of BALB / c mice using saponin as adjuvant, which allowed obtaining polyclonal antibodies capable of revealing fluorescein reaction in indirect immunofluorescence. The fusion of splenic cells, from the immunized mice with myeloma cells SP2 / 0 resulted in 72.4% hybridomas secreting anti-Nc-1antibodies. These hybridomas secreted antibodies positive to N. caninum and negative to Toxoplasma gondii.

KEYWORDS: hybridomas, immunodiagnosis, monoclonal antibodies, neosporosis.

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Author Biography

Bruna Alves Devens, Centro Universitário do Espírito Santo-UNESC

Médica Veterinária e mestre em medicina veterinária pela Universidade Federal de Viçosa. Professora Universitária dos cursos de saúde do UNESC. Médica Veterinária da Secretaria de Desenvolvimento Rural do Município de Colatina.

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Published

2014-06-29

How to Cite

DEVENS, B. A.; VARGAS VILORIA, M. I.; SOUZA E SILVA, C. H. de; SALCEDO, J. H. P. PRODUCTION OF HYBRIDOMAS SECRETING ANTI-Neospora caninum ANTIBODIES FOR USE IN IMMUNODIAGNOSTIC. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 15, n. 2, p. 228–233, 2014. DOI: 10.5216/cab.v15i2.23017. Disponível em: https://revistas.ufg.br/vet/article/view/23017. Acesso em: 27 jul. 2024.

Issue

Vol. 15 No. 2 (2014)

Section

Veterinary Medicine

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