Vibrio spp. ISOLATED FROM SHRIMPS AND WATER FROM A MARINE FARM IN PERNAMBUCO, BRAZIL

Abstract

Water and shrimp samples were collected monthly, during all cultivation phases, in three located farms at Pernambuco coast, on winter and summer, for Vibrio spp. quantification and identification. The counting’s were correlated, through mathematical models (P<0.05), with the variables season, water biochemical parameters, wet mount, histopathology exam, toxins presence and handling techniques used. Just the variable cultivation time interfered at total counting of Vibrio spp. in all samples were obtained countings that varied 0.1x 10 to 6.2 x 103 UFC/mL in water, of 7.0 x 10 to 8.2 x 105 UFC/g in powder-larva, of 1.1 x 10 to 1.1 x 105 UFC/mL in hemolymph and of 2.5 x 102 to 1.1 x 106UFC/g in hepatopancreas. The species V. mediterranei (1%), V. mimicus (1.25%), V. fischeri (4.25%), V. cincinnatiensis (4.25%), V. metschnikovii (4.25%), V. proteolyticus (5.5%), V. harveyi (5.5%), V. hollisae (5.5%), V. carchariae (7%), V. vulnificus (8.5%), V. damsela (8.5%), V. parahaemolyticus (13%), V. fluvialis (15%), V. anguillarum (16.5%) were identified. It is concluded that the Vibrionaceae load increases proportionally with cultivation time, due to the organic matter increment, what can turn the susceptible animals to the infection for vibrios.
KEY WORD: Vibrionaceae, Vibrio spp., shrimp and pond water.