EQUINE FROZEN SPERMATIC CELL SURVIVOR AFTER THAWED AND DILUTION USING TWO DIFFERENT COMMERCIAL EXTENDERS

Authors

  • Marina Ferreira Zimmermann Universidade de Brasília
  • Adalberto Farinasso
  • Cely Marini Melo UNESP/BOTUCATU
  • Frederico Ozanam Papa UNESP/BOTUCATU
  • Jairo Pereira Neves UNB

DOI:

https://doi.org/10.5216/cab.v10i1.1932

Keywords:

Reprodução Animal

Abstract

The purpose of this study was to evaluate the motility and viability of stallion semen cryopreserved with 5% dimethyl-formamyde as cryoprotectant and posterior dilution to 2.5 and 1.25% as final concentration of cryoprotectant. After thawing, samples were diluted with two commercial extenders (FR4® and Botucrio® ) in two moments: initial (Ti) and final (Tf). A total of 15 distinct ejaculates from five stallions of national breeds were analysed. Motility was observed using a computer assistant system analysis and viability was analyzed using fluorescent probes. A significant (P<0.05) increase on total and progressive motility parameters was observed at the final time using Botu-Crio®  compared with FR4®. Statistical difference (P>0.05) on membrane integrity between treatments was not observed.

KEY WORDS: Cryoprotectant,  diluent, equine, freezing, thawing.

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Author Biographies

Marina Ferreira Zimmermann, Universidade de Brasília

Faculdade de Agronomia e Medicina Veterinária; Mestrado em Ciências Agrárias, Produção Animal

Adalberto Farinasso

Laboratório Unicórnio de Reprodução Equina

Cely Marini Melo, UNESP/BOTUCATU

Doutoranda UNESP/BOTUCATU

Frederico Ozanam Papa, UNESP/BOTUCATU

Prof. Adjunto da área de Reprodução Animal da Faculdade de Medicina Veterinária e Zootecnia, UNESP, Campus Botucatu

Jairo Pereira Neves, UNB

Prof. Adjunto de Reprodução Animal da FAV/ UnB

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Published

2009-04-03

How to Cite

ZIMMERMANN, M. F.; FARINASSO, A.; MELO, C. M.; PAPA, F. O.; NEVES, J. P. EQUINE FROZEN SPERMATIC CELL SURVIVOR AFTER THAWED AND DILUTION USING TWO DIFFERENT COMMERCIAL EXTENDERS. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 10, n. 1, p. 238–245, 2009. DOI: 10.5216/cab.v10i1.1932. Disponível em: https://revistas.ufg.br/vet/article/view/1932. Acesso em: 30 jun. 2024.

Issue

Vol. 10 No. 1 (2009)

Section

Veterinary Medicine

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