EQUINE FROZEN SPERMATIC CELL SURVIVOR AFTER THAWED AND DILUTION USING TWO DIFFERENT COMMERCIAL EXTENDERS

Authors

  • Marina Ferreira Zimmermann Universidade de Brasília (UnB), Brasília, Distrito Federal, Brasil
  • Adalberto Farinasso
  • Cely Marini Melo Universidade Estadual Paulista (UNESP), Botucatu, São Paulo, Brasil
  • Frederico Ozanam Papa Universidade Estadual Paulista (UNESP), Botucatu, São Paulo, Brasil
  • Jairo Pereira Neves Universidade de Brasília (UnB), Brasília, Distrito Federal, Brasil

Keywords:

Reprodução Animal

Abstract

The purpose of this study was to evaluate the motility and viability of stallion semen cryopreserved with 5% dimethyl-formamyde as cryoprotectant and posterior dilution to 2.5 and 1.25% as final concentration of cryoprotectant. After thawing, samples were diluted with two commercial extenders (FR4® and Botucrio® ) in two moments: initial (Ti) and final (Tf). A total of 15 distinct ejaculates from five stallions of national breeds were analysed. Motility was observed using a computer assistant system analysis and viability was analyzed using fluorescent probes. A significant (P<0.05) increase on total and progressive motility parameters was observed at the final time using Botu-Crio®  compared with FR4®. Statistical difference (P>0.05) on membrane integrity between treatments was not observed.

KEY WORDS: Cryoprotectant,  diluent, equine, freezing, thawing.

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Published

2009-04-03

How to Cite

ZIMMERMANN, Marina Ferreira; FARINASSO, Adalberto; MELO, Cely Marini; PAPA, Frederico Ozanam; NEVES, Jairo Pereira. EQUINE FROZEN SPERMATIC CELL SURVIVOR AFTER THAWED AND DILUTION USING TWO DIFFERENT COMMERCIAL EXTENDERS. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 10, n. 1, p. 238–245, 2009. Disponível em: https://revistas.ufg.br/vet/article/view/1932. Acesso em: 14 dec. 2025.

Issue

Vol. 10 No. 1 (2009)

Section

Veterinary Medicine

License

Copyright (c) 2009 Brazilian Animal Science/ Ciência Animal Brasileira

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