USE OF ALTERNATIVE EXTENDERS AND TEMPERATURES IN LONG TERM STORAGE OF BOAR SEMEN

Authors

  • Lina Raquel Santos Araújo Faculdade de Veterinária da Universidade Estadual do Ceará
  • Tatyane Bandeira Barros Universidade Estadual do Ceará
  • Daianny Barboza Guimarães Curso de Ciências Biológicas da Universidade Estadual do Ceará
  • Ludymila Furtado Cantanhêde Universidade Estadual do Ceará
  • Aline Viana Dias Universidade Estadual do Ceará
  • Ricardo Toniolli Faculdade de Veterinária da Universidade Estadual do Ceará

DOI:

https://doi.org/10.1590/cab.v17i1.18885

Keywords:

Animal Reproduction

Abstract

The use of appropriate extenders is important for the success of an artificial insemination program. The objective of this study was to evaluate the efficiency of alternative extenders for swine semen at different temperatures (17 to 10 °C). The following extenders were used: Beltsville Thawing Solution (BTS), powdered coconut water (ACP-103®), and skimmed milk powder (LPD). The 50 ejaculates were analyzed daily, in natura and after dilution, during the 5-day period of semen preservation  (D0 to D4), regarding spermatic vigor and motility. Acrosome integrity and sperm viability were evaluated on D0 and D4. Data were analyzed using Mann-Whitney, Students, Tukey and chi-square tests (p<0.05). The LPD extender at 10 °C presented higher motility and sperm vigor compared to BTS and ACP until D2, and to treatments stored at 17 °C. Acrosome vitality and integrity remained higher (p<0.001) with LPD at 10 °C on D0 and D4. LPD showed to be a good extender for the swine semen at lower temperature (10 °C). Furthermore, it provided better protection to sperm cells, by allowing greater integrity and vitality of the acrosome.

Keywords: coconut water; conservation; skimmed milk; semen boar.

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Author Biographies

Lina Raquel Santos Araújo, Faculdade de Veterinária da Universidade Estadual do Ceará

Programa de Pós Graduação em Ciências Veterinárias (PPGCV)

Tatyane Bandeira Barros, Universidade Estadual do Ceará

Programa de Pós Graduação em Ciências Veterinárias (PPGCV)

Daianny Barboza Guimarães, Curso de Ciências Biológicas da Universidade Estadual do Ceará

Programa de Pós Graduação em Ciências Veterinárias (PPGCV)

Ludymila Furtado Cantanhêde, Universidade Estadual do Ceará

Programa de Pós Graduação em Ciências Veterinárias (PPGCV)

Aline Viana Dias, Universidade Estadual do Ceará

Programa de Pós Graduação em Ciências Veterinárias (PPGCV)

Ricardo Toniolli, Faculdade de Veterinária da Universidade Estadual do Ceará

Laboratório de Reprodução Suína e Tecnologia de Sêmen (LRSTS)

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Published

2016-01-30

How to Cite

ARAÚJO, L. R. S.; BARROS, T. B.; GUIMARÃES, D. B.; CANTANHÊDE, L. F.; DIAS, A. V.; TONIOLLI, R. USE OF ALTERNATIVE EXTENDERS AND TEMPERATURES IN LONG TERM STORAGE OF BOAR SEMEN. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 17, n. 1, p. 26–35, 2016. DOI: 10.1590/cab.v17i1.18885. Disponível em: https://revistas.ufg.br/vet/article/view/18885. Acesso em: 16 aug. 2024.

Issue

Vol. 17 No. 1 (2016)

Section

Animal Production

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