IDENTIFICATION OF BACTERIA IN THE SEMEN IN DIFFERENT BREEDING SYSTEMS AND THE EFFECT OF KILOL-L®

Authors

  • Gabriela de Oliveira Fernandes Universidade de Brasília
  • Diogo Ramos Leal Universidade de Brasília
  • Nathalia Hack Moreira Universidade de Brasília
  • Thiago A. de S. Nascimento Silva Universidade de Brasília
  • Alexandre Floriani Ramos Embrapa Cenargen
  • Jairo Pereira Neves Universidade de Brasília

DOI:

https://doi.org/10.5216/cab.v14i3.18613

Keywords:

Animal reproduction

Abstract

The objective of this study was to identify and quantify bacteria from fresh ram semen, evaluate the use of sanitizer Kilol-L® prior to semen collection, and test the sensitivity of bacterial strains against antibiotics. We selected 24 Santa Inês rams clinically healthy, at 4 years of aged, and grouped them into two systems: on pasture (n=12) and confined (n=12). The microbiological results indicated that of the total of 120 ejaculates, 99 had bacterial growth, representing 82.5% of the samples. The most frequently isolated bacteria were Bacillus spp., Corynebacterium spp., Escherichia coli, Listeria spp., Staphylococcus spp., and Streptococcus spp. From the tested antibiotics, amicacin and gentamicin were 100% effective for Bacillus spp. and E. Coli. Ceftiofur was effective for all isolates except for Rodococcus equi. Streptococcus spp. were susceptible to ampicillin and erythromycin and Staphylococcus spp. were sensitive to gentamycin. The use of Kilol-L® reduced the number of Colony Forming Units (CFU/mL) from ejaculate without damaging semen quality. From the tested antibiotics, ceftiofur and gentamicin were more effective against the isolated bacterial strains; thus the use of these antibiotics in the ram semen extender is an alternative to control bacterial growth.

KEYWORDS: antibiogram; bacteria; ovine; sanitizer; semen sperm parameters.

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Published

2013-09-28

How to Cite

FERNANDES, G. de O.; LEAL, D. R.; MOREIRA, N. H.; NASCIMENTO SILVA, T. A. de S.; RAMOS, A. F.; NEVES, J. P. IDENTIFICATION OF BACTERIA IN THE SEMEN IN DIFFERENT BREEDING SYSTEMS AND THE EFFECT OF KILOL-L®. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 14, n. 3, p. 332–337, 2013. DOI: 10.5216/cab.v14i3.18613. Disponível em: https://revistas.ufg.br/vet/article/view/18613. Acesso em: 22 nov. 2024.

Issue

Vol. 14 No. 3 (2013)

Section

Veterinary Medicine

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