INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN

Authors

  • Mauricio Fraga Van Tilburg Universidade Estadual do Norte Fluminense (UENF), Campos de Goytacazes, Rio de Janeiro, Brasil
  • José Frederico Straggiotti Silva Universidade Estadual do Norte Fluminense (UENF), Campos de Goytacazes, Rio de Janeiro, Brasil
  • Angelo José Burla Dias Universidade Estadual do Norte Fluminense (UENF), Campos de Goytacazes, Rio de Janeiro, Brasil
  • Célia Raquel Quirino Universidade Estadual do Norte Fluminense (UENF), Campos de Goytacazes, Rio de Janeiro, Brasil
  • Bruno Fagundes Universidade Estadual do Norte Fluminense (UENF), Campos de Goytacazes, Rio de Janeiro, Brasil

Abstract

Insulin is present on seminal plasma and it can be involved on sperm capacitation and sperm autocrine metabolism (AQUILA et al., 2005). It was verified the effect of insulin addition on ovine frozen semen extender. The parameters analyzed were sperm motility, sperm cinematic, acrossoma integrity and membrane functionality. Insulin was added to fraction B of TRIS-yolk extender in different concentrations. Sperm motility and cinematic were evaluated 0, 3 and 6 hours after thawing while acrossoma integrity and membrane functionality were evaluated 0 and 6 hours after thawing. The addition of 3 UI/mL of insulin increased amplitude lateral of head (5.42±0.97; C:4.75±0.47) and acrossoma integrity (153.67±16.98; C:134.00±18.08). The addition of 0.3 UI/mL of insulin improved retilinearity tree hours after thawing (83.17±2.71; C:78.67±4.97). It was verified a increase on linearity values on treatments with 0.3 e 3 UI/mL (52.17±2.14; 51.67±3.14; C:46.83±3.60), respectively tree hours after thawing. Treatment with 0.3 e 3 UI/ml showed higher acrossoma integrity than control (146.17±10.91; 146.33±16.02; C:117.00±17.80). Addiction of insulin on ovine frozen semen extender improved sperm motility, sperm cinematic and acrossoma integrity.

KEY WORDS: Freezing, insulin, ovine, spermetozoa.

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Published

2008-10-15

How to Cite

TILBURG, Mauricio Fraga Van; SILVA, José Frederico Straggiotti; DIAS, Angelo José Burla; QUIRINO, Célia Raquel; FAGUNDES, Bruno. INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 9, n. 3, p. 731–739, 2008. Disponível em: https://revistas.ufg.br/vet/article/view/1566. Acesso em: 6 dec. 2025.

Issue

Vol. 9 No. 3 (2008)

Section

Veterinary Medicine

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Copyright (c) 2008 Brazilian Animal Science/ Ciência Animal Brasileira

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