INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN

Authors

  • Mauricio Fraga van Tilburg UENF
  • José Frederico Straggiotti Silva UENF
  • Angelo José Burla Dias UENF
  • Célia Raquel Quirino UNEF
  • Bruno Fagundes UENF

DOI:

https://doi.org/10.5216/cab.v9i3.1566

Abstract

Insulin is present on seminal plasma and it can be involved on sperm capacitation and sperm autocrine metabolism (AQUILA et al., 2005). It was verified the effect of insulin addition on ovine frozen semen extender. The parameters analyzed were sperm motility, sperm cinematic, acrossoma integrity and membrane functionality. Insulin was added to fraction B of TRIS-yolk extender in different concentrations. Sperm motility and cinematic were evaluated 0, 3 and 6 hours after thawing while acrossoma integrity and membrane functionality were evaluated 0 and 6 hours after thawing. The addition of 3 UI/mL of insulin increased amplitude lateral of head (5.42±0.97; C:4.75±0.47) and acrossoma integrity (153.67±16.98; C:134.00±18.08). The addition of 0.3 UI/mL of insulin improved retilinearity tree hours after thawing (83.17±2.71; C:78.67±4.97). It was verified a increase on linearity values on treatments with 0.3 e 3 UI/mL (52.17±2.14; 51.67±3.14; C:46.83±3.60), respectively tree hours after thawing. Treatment with 0.3 e 3 UI/ml showed higher acrossoma integrity than control (146.17±10.91; 146.33±16.02; C:117.00±17.80). Addiction of insulin on ovine frozen semen extender improved sperm motility, sperm cinematic and acrossoma integrity.

KEY WORDS: Freezing, insulin, ovine, spermetozoa.

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Author Biographies

Mauricio Fraga van Tilburg, UENF

Médico veterinário formado pela Universidade Estadual do Norte Fluminense Darcy Ribeiro. Mestrado e Doutorado em Produção Animal na área de Biotecnologia da Reprodução pela Universidade Estadual do Norte Fluminense Darcy Ribeiro.

José Frederico Straggiotti Silva, UENF

Doutor em Medicina Veterinária pela Universidade de Hannover e professor associado da UENF.

Angelo José Burla Dias, UENF

Doutor em Produção Animal e professor associado da UENF.

Célia Raquel Quirino, UNEF

Doutora em Melhoramento Genético Animal e professora associada da UENF.

Bruno Fagundes, UENF

Mestre em Produção Animal e aluno de doutorado da UENF.

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Published

2008-10-15

How to Cite

VAN TILBURG, M. F.; SILVA, J. F. S.; DIAS, A. J. B.; QUIRINO, C. R.; FAGUNDES, B. INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 9, n. 3, p. 731–739, 2008. DOI: 10.5216/cab.v9i3.1566. Disponível em: https://revistas.ufg.br/vet/article/view/1566. Acesso em: 22 nov. 2024.

Issue

Vol. 9 No. 3 (2008)

Section

Veterinary Medicine

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