ANTIBACTERIAL EFFECT OF BRAZILIAN BROWN PROPOLIS IN DIFFERENT SOLVENTS AGAINST Staphylococcus spp. ISOLATED FROM CAPRINE MASTITIS

Authors

  • Carla S. Rodrigues Silva IF Sertão-PE, Campus Salgueiro
  • Claudia L. Paes Barreto IFCE, Campus Crato
  • Rodolfo de Moraes Peixoto IF Sertão-PE, Campus Floresta
  • Rinaldo Aparecido Mota UFRPE
  • Márcia de Fátima Ribeiro Embrapa Semiárido
  • Mateus Matiuzzi da Costa UNIVASF

DOI:

https://doi.org/10.5216/cab.v13i2.15121

Keywords:

Veterinary microbiology

Abstract

The purpose of the present study was to evaluate the antimicrobial activity of propolis extracts diluted in different solvents against bacteria from Staphylococcus genus. The study was performed in the Immunology and Microbiology Laboratory from Universidade Federal do Vale do São Francisco. The propolis extracts were prepared using brown propolis diluted in different solvents such as chloroform, methanol, ethyl acetate and grain alcohol. In order to determine the antimicrobial potential of extracts, agar well diffusion method was used, with controls for each diluent. After that, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) methods were used. All tests were performed in triplicate. In the agar well diffusion test, the measurements of the inhibition zone for propolis extract were as follows:  grain alcohol and propolis (2.88mm), methanol and propolis (2.41mm), chloroform and propolis (2.40mm) and ethyl acetate and propolis (0.83mm). The MBC of propolis extracts in different solvents were 93.75 ?g/mL for grain alcohol, 375 ?g/mL for chloroform and methanol and 3,000 ?g/ml for ethyl acetate. Statistically significant differences were achieved comparing the inhibition zones of propolis diluted in grain alcohol and ethyl acetate (2.88 and 0.83 mm, respectively). Considering the low cost of therapy and the activity of the propolis against caprine mastitis pathogens, other studies regarding in vivo activity and chemical characterization are necessary, in addition to evaluation of the toxicological aspects of propolis extracts.

KEYWORDS: Antimicrobial therapy; dairy goats; minimal bactericidal concentration; natural products.

Downloads

Download data is not yet available.

Downloads

Published

2012-06-27

How to Cite

SILVA, C. S. R.; PAES BARRETO, C. L.; PEIXOTO, R. de M.; MOTA, R. A.; RIBEIRO, M. de F.; DA COSTA, M. M. ANTIBACTERIAL EFFECT OF BRAZILIAN BROWN PROPOLIS IN DIFFERENT SOLVENTS AGAINST Staphylococcus spp. ISOLATED FROM CAPRINE MASTITIS. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 13, n. 2, p. 247–251, 2012. DOI: 10.5216/cab.v13i2.15121. Disponível em: https://revistas.ufg.br/vet/article/view/15121. Acesso em: 16 aug. 2024.

Issue

Vol. 13 No. 2 (2012)

Section

Veterinary Medicine

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).