APPLICATION OF POLIMERASE CHAIN REACTION FOR DETECTION OF Mycoplasma spp IN THE ROUTINE OF CELL CULTURES

Authors

  • Marcelo Fernandes Camargos MAPA-Laboratório Nacional Agropecuário-MG
  • Anapolino Macedo de Oliveira
  • Antônio Augusto Fonseca Júnior
  • Anselmo Vasconcelos Rivetti
  • Pedro Moreira Couto Motta
  • Ronnie Antunes Assis
  • Rômulo Cerqueira Leite

DOI:

https://doi.org/10.5216/cab.v9i3.1400

Abstract

The occurrence of Mycoplasma spp as contaminant of cell cultures in cell culture laboratories of the Escola de Veterinária da UFMG and of the Laboratório Nacional Agropecuário de Minas Gerais was obtained by the use of a fast DNA extraction protocol e by the use of PCR. The PCR sensitivity and specificity were evaluated. The search for Mycoplasmas DNA was done in 83 cell lines samples. We found infected cell lines in both laboratories. The quality of extracted DNA was verified using a beta-actin gene PCR. The Mycoplasmas’s PCR showed to be fast, sensitive and specific.

 

KEY WORDS: Beta-actin, cell, culture, Mycoplasma spp, PCR.

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Author Biography

Marcelo Fernandes Camargos, MAPA-Laboratório Nacional Agropecuário-MG

Laboratório de Biossegurança nível 3+ / Laboratório de Diagóstico de Doenças Virais

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Published

2008-10-15

How to Cite

CAMARGOS, M. F.; OLIVEIRA, A. M. de; FONSECA JÚNIOR, A. A.; RIVETTI, A. V.; MOTTA, P. M. C.; ASSIS, R. A.; LEITE, R. C. APPLICATION OF POLIMERASE CHAIN REACTION FOR DETECTION OF Mycoplasma spp IN THE ROUTINE OF CELL CULTURES. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 9, n. 3, p. 786–790, 2008. DOI: 10.5216/cab.v9i3.1400. Disponível em: https://revistas.ufg.br/vet/article/view/1400. Acesso em: 25 nov. 2024.

Issue

Vol. 9 No. 3 (2008)

Section

Veterinary Medicine

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