IN VITRO CHARACTERISTICS AND FERTILITY OF GOAT SEMEN COOLED AT 5°C FOR 24 HOURS USING TWO EGG YOLK CONCENTRATIONS IN SEMEN EXTENDER

Authors

  • Charles André S. Bispo Instituto Federal de Minas Gerais (IFMG), São João Evangelista, Minas Gerais, Brasi
  • Guilherme Pugliesi Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brasil
  • Miller Pereira Palhão Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Embrapa Gado de Leite, Juiz de Fora, Minas Gerais, Brasil
  • Polyana Galvão B. Coelho Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brasil
  • Pedro Gama Ker Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brasil
  • Marcelo Teixeira Rodrigues Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brasil
  • Giovanni Ribeiro Carvalho Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brasil

DOI:

https://doi.org/10.5216/cab.v12i4.13115

Keywords:

Animal Reproduction

Abstract

The objective of this study was to evaluate by in vitro tests and in vivo fertility, two concentrations of egg yolk (T1 = 20% or T2 = 2.5%) in egg yolk-citrate semen extender for storing goat semen at 5°C for 24 hours. Ninety semen collections were performed in four goats. The semen was split and diluted in two different semen extenders and stored in refrigerator at 5°C for 24 hours, until the semen evaluation and insemination procedures. The average values for cooled semen, respectively, for T1 and T2 were motility, 68.5 ± 15.4 and 78.0 ± 5.5 (p < 0.05); vigor, 2.5 ± 0.6 and 3.2 ± 0.3 (p < 0.05), supravital test, 59.4 ± 17.2 and 71.1 ± 10.5 (p < 0.05) and hypoosmotic test, 42.2 ± 15.7 and 56.2 ± 11.5 (p < 0.05). The fertility rate was greater (p < 0.05) in T1 (31.3%) than in T2 (66.7%) and the prolificacy did not differ (p < 0.05) between T1 (1.3 ± 0.5) and T2 (1.4 ± 0.5). In conclusion, the egg yolk-citrate extender with a low concentration (2.5%) of egg yolk was more effective in preserving the viability and fertility of goat semen stored at 5°C for 24 hours.

KEYWORDS: artificial insemination; extender; goat; semen; spermatozoa.

Downloads

Download data is not yet available.

Downloads

Published

2011-12-20

How to Cite

BISPO, Charles André S.; PUGLIESI, Guilherme; PALHÃO, Miller Pereira; COELHO, Polyana Galvão B.; KER, Pedro Gama; RODRIGUES, Marcelo Teixeira; CARVALHO, Giovanni Ribeiro. IN VITRO CHARACTERISTICS AND FERTILITY OF GOAT SEMEN COOLED AT 5°C FOR 24 HOURS USING TWO EGG YOLK CONCENTRATIONS IN SEMEN EXTENDER. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 12, n. 4, p. 653–660, 2011. DOI: 10.5216/cab.v12i4.13115. Disponível em: https://revistas.ufg.br/vet/article/view/13115. Acesso em: 5 dec. 2025.

Issue

Vol. 12 No. 4 (2011)

Section

Animal Production

License

Copyright (c) 2011 Brazilian Animal Science/ Ciência Animal Brasileira

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
Crossref
Scopus
Google Scholar
Europe PMC