IN VITRO CHARACTERISTICS AND FERTILITY OF GOAT SEMEN COOLED AT 5°C FOR 24 HOURS USING TWO EGG YOLK CONCENTRATIONS IN SEMEN EXTENDER

Authors

  • Charles André S. Bispo Instituto Federal de Minas Gerais
  • Guilherme Pugliesi Universidade Federal de Viçosa
  • Miller Pereira Palhão Empresa Brasileira de Pesquisa Agropecuária
  • Polyana Galvão B. Coelho Universidade Federal de Viçosa
  • Pedro Gama Ker Universidade Federal de Viçosa
  • Marcelo Teixeira Rodrigues Universidade Federal de Viçosa
  • Giovanni Ribeiro Carvalho Universidade Federal de Viçosa

DOI:

https://doi.org/10.5216/cab.v12i4.13115

Keywords:

Animal Reproduction

Abstract

The objective of this study was to evaluate by in vitro tests and in vivo fertility, two concentrations of egg yolk (T1 = 20% or T2 = 2.5%) in egg yolk-citrate semen extender for storing goat semen at 5°C for 24 hours. Ninety semen collections were performed in four goats. The semen was split and diluted in two different semen extenders and stored in refrigerator at 5°C for 24 hours, until the semen evaluation and insemination procedures. The average values for cooled semen, respectively, for T1 and T2 were motility, 68.5 ± 15.4 and 78.0 ± 5.5 (p < 0.05); vigor, 2.5 ± 0.6 and 3.2 ± 0.3 (p < 0.05), supravital test, 59.4 ± 17.2 and 71.1 ± 10.5 (p < 0.05) and hypoosmotic test, 42.2 ± 15.7 and 56.2 ± 11.5 (p < 0.05). The fertility rate was greater (p < 0.05) in T1 (31.3%) than in T2 (66.7%) and the prolificacy did not differ (p < 0.05) between T1 (1.3 ± 0.5) and T2 (1.4 ± 0.5). In conclusion, the egg yolk-citrate extender with a low concentration (2.5%) of egg yolk was more effective in preserving the viability and fertility of goat semen stored at 5°C for 24 hours.

KEYWORDS: artificial insemination; extender; goat; semen; spermatozoa.

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Author Biographies

Charles André S. Bispo, Instituto Federal de Minas Gerais

Professor do Instituto Federal Minas Gerais - Campus São João Evangelista, Minas Gerais.

Guilherme Pugliesi, Universidade Federal de Viçosa

Doutorando em Reprodução Animal, Departamento de Zootecnia

Miller Pereira Palhão, Empresa Brasileira de Pesquisa Agropecuária

Pós-Doutorando. Empresa Brasileira de Pesquisa Agropecuária - Embrapa Gado de Leite, Juiz de Fora, Minas Gerais

Polyana Galvão B. Coelho, Universidade Federal de Viçosa

Mestranda em Reprodução Animal, Departamento de Veterinária

Pedro Gama Ker, Universidade Federal de Viçosa

Doutorando em Reprodução Animal, Departamento de Zootecnia

Marcelo Teixeira Rodrigues, Universidade Federal de Viçosa

Professor do Departamento de Zootecnia da Universidade Federal de Viçosa

Giovanni Ribeiro Carvalho, Universidade Federal de Viçosa

Professor do Departamento de Zootecnia da Universidade Federal de Viçosa

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Published

2011-12-20

How to Cite

BISPO, C. A. S.; PUGLIESI, G.; PALHÃO, M. P.; COELHO, P. G. B.; KER, P. G.; RODRIGUES, M. T.; CARVALHO, G. R. IN VITRO CHARACTERISTICS AND FERTILITY OF GOAT SEMEN COOLED AT 5°C FOR 24 HOURS USING TWO EGG YOLK CONCENTRATIONS IN SEMEN EXTENDER. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 12, n. 4, p. 653–660, 2011. DOI: 10.5216/cab.v12i4.13115. Disponível em: https://revistas.ufg.br/vet/article/view/13115. Acesso em: 30 jun. 2024.

Issue

Vol. 12 No. 4 (2011)

Section

Animal Production

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