IN VITRO CHARACTERISTICS AND FERTILITY OF GOAT SEMEN COOLED AT 5°C FOR 24 HOURS USING TWO EGG YOLK CONCENTRATIONS IN SEMEN EXTENDER
DOI:
https://doi.org/10.5216/cab.v12i4.13115Keywords:
Animal ReproductionAbstract
The objective of this study was to evaluate by in vitro tests and in vivo fertility, two concentrations of egg yolk (T1 = 20% or T2 = 2.5%) in egg yolk-citrate semen extender for storing goat semen at 5°C for 24 hours. Ninety semen collections were performed in four goats. The semen was split and diluted in two different semen extenders and stored in refrigerator at 5°C for 24 hours, until the semen evaluation and insemination procedures. The average values for cooled semen, respectively, for T1 and T2 were motility, 68.5 ± 15.4 and 78.0 ± 5.5 (p < 0.05); vigor, 2.5 ± 0.6 and 3.2 ± 0.3 (p < 0.05), supravital test, 59.4 ± 17.2 and 71.1 ± 10.5 (p < 0.05) and hypoosmotic test, 42.2 ± 15.7 and 56.2 ± 11.5 (p < 0.05). The fertility rate was greater (p < 0.05) in T1 (31.3%) than in T2 (66.7%) and the prolificacy did not differ (p < 0.05) between T1 (1.3 ± 0.5) and T2 (1.4 ± 0.5). In conclusion, the egg yolk-citrate extender with a low concentration (2.5%) of egg yolk was more effective in preserving the viability and fertility of goat semen stored at 5°C for 24 hours.
KEYWORDS: artificial insemination; extender; goat; semen; spermatozoa.
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