EVALUATION OF AGAR GEL MICROIMUNODIFUSION FOR DIAGNOSIS OF SMALL RUMINANT LENTIVIRUSES (SRLV) IN GOATS

Authors

  • Emmanuela T. de Arruda Universidade Federal Rural de Pernambuco
  • Michele M. Martins Oliveira Faculdade de Imperatriz (FACIMP)
  • Sérgio Alves do Nascimento Universidade Federal Rural de Pernambuco
  • Ana Claudia Campos Universidade Federal Rural de Pernambuco
  • Roberto Soares de Castro Universidade Federal Rural de Pernambuco

DOI:

https://doi.org/10.5216/cab.v12i3.12475

Keywords:

Animal sanity

Abstract

Small Ruminant Lentiviruses - SRLV (Caprine Arthritis - encephalitis - CAE and Maedi-Visna) infections are characterized by slow and progressive course, in which the majority of the animals do not present symptoms. Thus, the serological tests are the most practical diagnostic tool as the presence of antibodies indirectly indicates SRLV infection. This work was carried out with the objective of evaluating an agar gel micro-immunodifusion (micro-AGID) test in comparison with a macro-AGID test to be used in the serological diagnostic of SRLV in goats. In the micro-AGID, 10 ?L of antigen and control serum and 30 ?L of test serum were used; in the macro-AGID, 20 ?L of sera and antigen were used. The tests were compared through the adjusted agreement index (Kappa - k) calculated according to 447 samples of goat serum from Pernambuco, Paraíba and Rio Grande do Norte states. Of 447 samples, 63 (14.09%) and 60 (13.42%) were positive to micro-AGID and macro-AGID, respectively, corresponding to a significant agreement between the tests (k = 0.90). Furthermore, the micro-AGID presented clearer lines than the macro-AGID and the results may be recorded 24 earlier in micro-AGID. Thus, the micro-AGID may replace the macro-AGID in the serological SRL diagnosis.
KEYWORDS: AGID; CAEV; diagnostic; Goat; Maedi-Visna.

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Author Biographies

Emmanuela T. de Arruda, Universidade Federal Rural de Pernambuco

´Mestre em Ciência Animal

Michele M. Martins Oliveira, Faculdade de Imperatriz (FACIMP)

Professora Doutora da Faculdade de Imperatriz (FACIMP)

Sérgio Alves do Nascimento, Universidade Federal Rural de Pernambuco

Biológo da Universidade Federal Rural de Pernambuco

Ana Claudia Campos, Universidade Federal Rural de Pernambuco

Doutoranda do Programa de Pós-graduação em Ciência Veterinária da Universidade Federal Rural de Pernambuco (UFRPE)

Roberto Soares de Castro, Universidade Federal Rural de Pernambuco

Professor Associado da Universidade Federal Rural de Pernambuco (UFRPE)

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Published

2011-09-29

How to Cite

ARRUDA, E. T. de; OLIVEIRA, M. M. M.; NASCIMENTO, S. A. do; CAMPOS, A. C.; CASTRO, R. S. de. EVALUATION OF AGAR GEL MICROIMUNODIFUSION FOR DIAGNOSIS OF SMALL RUMINANT LENTIVIRUSES (SRLV) IN GOATS. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 12, n. 3, p. 560–565, 2011. DOI: 10.5216/cab.v12i3.12475. Disponível em: https://revistas.ufg.br/vet/article/view/12475. Acesso em: 17 may. 2024.

Issue

Vol. 12 No. 3 (2011)

Section

Veterinary Medicine

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