COMPARISON BETWEEN THE CONVENTIONAL AND AUTOMATED SYSTEMS OF SEMEN BOVINE CRYOPRESERVATION

Authors

  • Cátia O. Guimarães Abud Escola de Veterinária-UFG
  • Lucas Jacomini Abud Professor da Universidade Federal Rural da Amazônia
  • José Carvalho Oliveira Neto Universidade de São Paulo – Campus Pirassununga
  • Margot Alves Nunes Dode da Embrapa Recursos Genéticos e Biotecnologia
  • José Robson Bezerra Sereno Embrapa Cerrados
  • Carlos Frederico Martins Embrapa Cerrados

DOI:

https://doi.org/10.5216/cab.v15i1.12233

Keywords:

Animal Reproduction

Abstract

The objective of this study was to compare the efficiency of bovine semen cryopreservation using the controlled-rate freezing machine versus the conventional method (uncontrolled curve) by the parameters of sperm quality and viability in post-thaw period. Semen from four adult crossbreed bulls was cryopreserved in Tris-yolk-glycerol medium. The computer assisted analysis of thawed semen detected the following results: PM 56.50±22.25%; VAP 34.77±4.25?m/s; VSL 28.17±4.25?m/s; VCL 58.45±6.85?m/s; STR 82.00±2.31%; LIN 49.50±3.32%, to automated system and PM 57.00±13.11%; VAP 25.75±1.66?m/s; VSL 23.32±1.99?m/s; VCL 63.32±1.79?m/s; STR 82.25±3.59?m/s; LIN 50.00±4.97?m/s to conventional cryopreservation system. The results of plasma membrane and acrosome integrity evaluation were 54.7±12.55% and 36.13±22.20% for the automated system and 53.22±13.22% and 47.26±5.74% for the conventional system, respectively. The parameters evaluated demonstrated that there was no statistical difference between the cryopreservation systems. Thus, the choice of the bovine semen cryopreservation method to be used on a farm is a responsibility of the technician, and should be based on the reality of each farm. Therefore, it is always necessary to consider that the conventional system of bovine semen cryopreservation can vary more than the automated system, which, despite the cost of the equipment, can ensure repeatability of the results and consequent quality of cryopreserved bovine semen.

KEYWORDS: artificial insemination; biotechnology; bovine sperm; cryopreservation; cryopreservation curve.

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Published

2014-03-28

How to Cite

ABUD, C. O. G.; ABUD, L. J.; OLIVEIRA NETO, J. C.; NUNES DODE, M. A.; SERENO, J. R. B.; MARTINS, C. F. COMPARISON BETWEEN THE CONVENTIONAL AND AUTOMATED SYSTEMS OF SEMEN BOVINE CRYOPRESERVATION. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 15, n. 1, p. 32–37, 2014. DOI: 10.5216/cab.v15i1.12233. Disponível em: https://revistas.ufg.br/vet/article/view/12233. Acesso em: 17 may. 2024.

Issue

Vol. 15 No. 1 (2014)

Section

Animal Production

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