EFFECT OF IMPERMEANT CRYOPROTECTANTS ON THE IN VITRO VIABILITY OF FROZEN SPERMATOZOA OF SWISS-ALBINA AND BALB/C MICE (Mus musculus)

Authors

  • Ligia Maria Piassi Universidade Federal de Pelotas
  • Carine Dahl Corcini
  • Bruna da Rosa Curcio
  • Andrea Panzardi
  • Naina Oliveira Martins
  • Milton Oliveira Amado
  • Antonio Sergio Varela Junior
  • Thomaz Lucia Jr
  • João Carlos Deschamps

DOI:

https://doi.org/10.5216/cab.v10i4.1200

Keywords:

Animal sanity

Abstract

The objective of this study was to evaluate the effect of three disaccharides (sucrose, threalose and lactose) used as nonpenetrating cryoprotectants in extender for mice semen. The parameters evaluated were sperm motility (MOT) and cleavage rate (CLV) after in vitro fertilization in the SWISS-ALBINA and BALB/c lines. The treatments were S1=sucrose; S2=threalose; S3=lactose, for SWISS-ALBINA; and B1=lactose; B2=threalose for BALB/c.  MOT was evaluated after: thawing (THA), centrifugation and re-suspension in P-1 medium (CEN) and after 10 minutes of incubation (10M). The MOT for the SWISS-ALBINA line was higher for S2 (P<0.001) in the 3 evaluated steps (47% at DES; 66.5% at CEN and 67.2% at 10M) than for S1 (32.5% at DES; 51.5% at CEN and 47.7% at 10M) and S3 (30% at DES, 46.5% at CEN and 32.7% at 10M). For the BALB/c line, MOT was superior for B2 than for B1 (P<0.001). Thus, the tested disaccharides, especially threalose, can be recommended for freezing of mice sperm.
KEY WORDS: Cryopreservation, disaccharides, mice, semen. 

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Author Biographies

Ligia Maria Piassi, Universidade Federal de Pelotas

Médico Veterinária, M.C

Carine Dahl Corcini

Médica Veterinária

Bruna da Rosa Curcio

Médica Veteirária, M.C., D.C Reprodução Animal

Andrea Panzardi

Médica Veterinária, M.C

Naina Oliveira Martins

Estudante Medicina Veterinária

Milton Oliveira Amado

Médico Veterinário

Antonio Sergio Varela Junior

Médico Veterinário, M.C.

Thomaz Lucia Jr

Médico Veterinário, M.C., PhD

João Carlos Deschamps

Médico Veterinário, M.Sc.,PhD Área Reprodução Animal

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Published

2009-12-17

How to Cite

PIASSI, L. M.; CORCINI, C. D.; CURCIO, B. da R.; PANZARDI, A.; MARTINS, N. O.; AMADO, M. O.; VARELA JUNIOR, A. S.; LUCIA JR, T.; DESCHAMPS, J. C. EFFECT OF IMPERMEANT CRYOPROTECTANTS ON THE IN VITRO VIABILITY OF FROZEN SPERMATOZOA OF SWISS-ALBINA AND BALB/C MICE (Mus musculus). Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 10, n. 4, p. 1161–1170, 2009. DOI: 10.5216/cab.v10i4.1200. Disponível em: https://revistas.ufg.br/vet/article/view/1200. Acesso em: 30 jun. 2024.

Issue

Vol. 10 No. 4 (2009)

Section

Veterinary Medicine

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