Avaliação morfológica de córneas de coelhos criopreservadas com etilenoglicol

Lorena Borges Alves; Duvaldo Eurides; Lilian Vieira de Andrade; Luiz Antônio F. da Silva; Benito J. N. Alves de Oliveira; Luiz Augusto de Souza

doi:10.5216/cab.v12i2.11445

MORPHOLOGICAL EVALUATION OF RABBIT CORNEA CRYOPRESERVED WITH ETHYLENE GLYCOL

Authors

Lorena Borges Alves Universidade Federal de Uberlândia (UFU), Uberlândia, Minas Gerais, Brasil
Duvaldo Eurides Universidade Federal de Uberlândia (UFU), Uberlândia, Minas Gerais, Brasil
Lilian Vieira de Andrade Universidade Federal de Uberlândia (UFU), Uberlândia, Minas Gerais, Brasil
Luiz Antônio F. da Silva Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil
Benito J. N. Alves de Oliveira Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil
Luiz Augusto de Souza Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil

DOI:

https://doi.org/10.5216/cab.v12i2.11445

Keywords:

Animal sanity

Abstract

Rabbit corneas, cryopreserved with ethylene glycol, were evaluated. Corneas of the right and left ocular globes were collected from eleven New Zelanda male rabbits, weighing between 4.0kg and 5.0kg, immediately after the animals were slaughtered. The corneas were removed with a 2.0mm edge of sclera and washed in physiological solution 0.9%. The right corneas were fixed in formaldeide, enclosed in paraffin, stained in hematoxyline eosine for evaluation in light microscopy. The left corneas were immersed in cryoprotect bottles containing phosphate-buffered saline solution, 0.4% of bovine albumen and 1.5 molar of ethylene glycol. The bottles were frozen for 60 days at -196º C in liquid nitrogen. After thawing, they were submitted to macrocospic analysis, and measured from digital images. The rabbits´ corneas preservated with ethylene glycol in liquid nitrogen caused no structural nor morphometry alterations at the layers that compose the cornea.

KEYWORDS: córnea; cryopreserved; rabbit.

Downloads

Download data is not yet available.

Author Biographies

Lorena Borges Alves, Universidade Federal de Uberlândia (UFU), Uberlândia, Minas Gerais, Brasil

Benito J. N. Alves de Oliveira, Universidade Federal de Goiás (UFG), Goiânia, Goiás, Brasil

Downloads

Published

2011-06-25

How to Cite

ALVES, Lorena Borges; EURIDES, Duvaldo; ANDRADE, Lilian Vieira de; SILVA, Luiz Antônio F. da; OLIVEIRA, Benito J. N. Alves de; SOUZA, Luiz Augusto de. MORPHOLOGICAL EVALUATION OF RABBIT CORNEA CRYOPRESERVED WITH ETHYLENE GLYCOL. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 12, n. 2, p. 376–381, 2011. DOI: 10.5216/cab.v12i2.11445. Disponível em: https://revistas.ufg.br/vet/article/view/11445. Acesso em: 5 dec. 2025.

Download Citation

Issue

Vol. 12 No. 2 (2011)

Section

Veterinary Medicine

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).