MORPHOLOGICAL EVALUATION OF RABBIT CORNEA CRYOPRESERVED WITH ETHYLENE GLYCOL

Authors

  • Lorena Borges Alves FAMEV/UFU
  • Duvaldo Eurides Universidade Federal de Uberlândia
  • Lilian Vieira de Andrade FAMEV/UFU
  • Luiz Antônio F. da Silva EV/UFG
  • Benito J. N. Alves de Oliveira EV/UFG
  • Luiz Augusto de Souza EV/UFG

DOI:

https://doi.org/10.5216/cab.v12i2.11445

Keywords:

Animal sanity

Abstract

Rabbit corneas, cryopreserved with ethylene glycol, were evaluated. Corneas of the right and left ocular globes were collected from eleven New Zelanda male rabbits, weighing between 4.0kg and 5.0kg, immediately after the animals were slaughtered. The corneas were removed with a 2.0mm edge of sclera and washed in physiological solution 0.9%. The right corneas were fixed in formaldeide, enclosed in paraffin, stained in hematoxyline eosine for evaluation in light microscopy. The left corneas were immersed in cryoprotect bottles containing phosphate-buffered saline solution, 0.4% of bovine albumen and 1.5 molar of ethylene glycol. The bottles were frozen for 60 days at -196º C in liquid nitrogen. After thawing,  they were submitted to macrocospic analysis, and measured from digital images. The rabbits´ corneas preservated with ethylene glycol in liquid nitrogen caused no structural nor morphometry alterations at the layers that compose the cornea.

KEYWORDS: córnea; cryopreserved; rabbit.

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Author Biographies

Lorena Borges Alves, FAMEV/UFU


Duvaldo Eurides, Universidade Federal de Uberlândia

Médico Veterinário. Prof. Dr. Faculdade de Medicina Veterinária - Universidade Federal de Uberlândia

Lilian Vieira de Andrade, FAMEV/UFU

Médico Veterinário. Prof. Dr. Faculdade de Medicina Veterinária - Universidade Federal de Uberlândia

Luiz Antônio F. da Silva, EV/UFG

Professor Doutor EV/UFG

Benito J. N. Alves de Oliveira, EV/UFG


Luiz Augusto de Souza, EV/UFG

Médicos Veterinários. Alunos do Programa de Pós-graduação Saúde Animal/EV/UFG.

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Published

2011-06-25

How to Cite

ALVES, L. B.; EURIDES, D.; ANDRADE, L. V. de; SILVA, L. A. F. da; OLIVEIRA, B. J. N. A. de; SOUZA, L. A. de. MORPHOLOGICAL EVALUATION OF RABBIT CORNEA CRYOPRESERVED WITH ETHYLENE GLYCOL. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 12, n. 2, p. 376–381, 2011. DOI: 10.5216/cab.v12i2.11445. Disponível em: https://revistas.ufg.br/vet/article/view/11445. Acesso em: 22 nov. 2024.

Issue

Vol. 12 No. 2 (2011)

Section

Veterinary Medicine

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