OBTENTION METHODS OF RESPIRATORY TRACT CELSS IN HEALTHY SHEEP BY THE TRACHEOBRONCHEAL LAVAGE TECHNIQUE BY NASOTRACHEAL VIA

Authors

  • Julio Simões Marcondes Faculdade de Medicina Veterinária e Zootecnia - Unesp Botucatu
  • Roberto Calderon Gonçalves Faculdade de Medicina Veterinária e Zootecnia - Unesp Botucatu

DOI:

https://doi.org/10.5216/cab.v9i2.1049

Keywords:

Sanidade Animal, ovinos, lavado traqueobrônquico, citologia

Abstract

The technique of pulmonary cells obtention by nasotracheal intubation was evaluated and the cellular population of 19 healthy sheep was characterized. The animals were hold standing still with head and neck strait; aligned with the vertebral spine and a silicone tube guide till the carina, to introduce a lower caliber tube for precede the tracheobronchial lavage. The average counting of nucleated cells was 64.650 ± 49.674. The cytological analyses of the samples slowed by the average of obtained percentages: 62.74% of macrophages, 15.01% cylindrical epithelial cells, 20.04% of neutrophills, 1.44 of lymphocytes and 0.77% of eosinophills. It was concluded that this methods of sample obtention by nasotracheal intubation to reach the tracheobronchial region was efficient to cytological characterization and cellular differentiation in the obtained samples, being well supported by the animals.

 

KEY WORDS: Citocentrifugation, cytology, tracheobronchial lavage, sheep.

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Author Biographies

Julio Simões Marcondes, Faculdade de Medicina Veterinária e Zootecnia - Unesp Botucatu

Pós graduando da Área de Clínica de Grandes Animais da Faculdade de Medicina Veterinária e Zootecnia – FMVZ, Botucatu – Universidade Estadual Paulista. Departamento de Clínica Veterinária

Roberto Calderon Gonçalves, Faculdade de Medicina Veterinária e Zootecnia - Unesp Botucatu

Prof° Ass. Dr. da área de Clínica de Grandes Animais da Faculdade de Medicina Veterinária e Zootecnia – FMVZ, Botucatu – Universidade Estadual Paulista. Departamento de Clínica Veterinária.

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Published

2008-07-09

How to Cite

MARCONDES, J. S.; GONÇALVES, R. C. OBTENTION METHODS OF RESPIRATORY TRACT CELSS IN HEALTHY SHEEP BY THE TRACHEOBRONCHEAL LAVAGE TECHNIQUE BY NASOTRACHEAL VIA. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 9, n. 2, p. 418–426, 2008. DOI: 10.5216/cab.v9i2.1049. Disponível em: https://revistas.ufg.br/vet/article/view/1049. Acesso em: 21 nov. 2024.

Issue

Vol. 9 No. 2 (2008)

Section

Veterinary Medicine

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