OBTENTION METHODS OF RESPIRATORY TRACT CELSS IN HEALTHY SHEEP BY THE TRACHEOBRONCHEAL LAVAGE TECHNIQUE BY NASOTRACHEAL VIA

Authors

  • Julio Simões Marcondes Universidade Estadual Paulista (UNESP), Botucatu, São Paulo, Brasil
  • Roberto Calderon Gonçalves Universidade Estadual Paulista (UNESP), Botucatu, São Paulo, Brasil

Keywords:

Sanidade Animal, ovinos, lavado traqueobrônquico, citologia

Abstract

The technique of pulmonary cells obtention by nasotracheal intubation was evaluated and the cellular population of 19 healthy sheep was characterized. The animals were hold standing still with head and neck strait; aligned with the vertebral spine and a silicone tube guide till the carina, to introduce a lower caliber tube for precede the tracheobronchial lavage. The average counting of nucleated cells was 64.650 ± 49.674. The cytological analyses of the samples slowed by the average of obtained percentages: 62.74% of macrophages, 15.01% cylindrical epithelial cells, 20.04% of neutrophills, 1.44 of lymphocytes and 0.77% of eosinophills. It was concluded that this methods of sample obtention by nasotracheal intubation to reach the tracheobronchial region was efficient to cytological characterization and cellular differentiation in the obtained samples, being well supported by the animals.

 

KEY WORDS: Citocentrifugation, cytology, tracheobronchial lavage, sheep.

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Published

2008-07-09

How to Cite

MARCONDES, Julio Simões; GONÇALVES, Roberto Calderon. OBTENTION METHODS OF RESPIRATORY TRACT CELSS IN HEALTHY SHEEP BY THE TRACHEOBRONCHEAL LAVAGE TECHNIQUE BY NASOTRACHEAL VIA. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 9, n. 2, p. 418–426, 2008. Disponível em: https://revistas.ufg.br/vet/article/view/1049. Acesso em: 11 feb. 2026.

Issue

Vol. 9 No. 2 (2008)

Section

Veterinary Medicine

License

Copyright (c) 2008 Brazilian Animal Science/ Ciência Animal Brasileira

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