IN VITRO ACTIVITY OF SECONDARY METABOLITES OF Burkholderia sp. (BURKHOLDER 1950) AGAINST THE YEAST Malassezia pachydermatis (WEIDMANN) DODGE 1935.

Authors

  • Olney Vieira-da-Motta Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brasil
  • Maria José Ribeiro da Silva Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brasil
  • Mariana Feitosa de Deus Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brasil
  • Richard Ian Samuels Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brasil
  • Luciana Mathias Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brasil
  • João Carlos de Aquino Almeida Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro, Brasil
  • Erick Vaz Guimarães Fiocruz
  • Antonio Henrique Almeida Moraes-Neto Fiocruz

Keywords:

Sanidade Animal

Abstract

M. pachydermatis is among the main agents involved in skin lesion and otopathological alterations of dogs and cats frequently associated to bacterial infections and host immune suppression. Because of resistance toward drugs, the market urge new therapies for treatment of fungal infections are needed. The effect of metabolites present in the filtrate culture of Burkholderia sp. (FCB) was tested against native clinical strains of the yeast M. pachydermatis in liquid medium measured by standard growth curve, by agar diffusion method, and colony forming units count in agar. Activity of FCB on cells was evaluated by transmission eletron microscopy (TEM). For comparison, sensitivity tests were performed on the yeast by diffusion agar test using commercial drugs. Crude FCB presented activity against the yeast by the methods utilized. The activity of FCB on cells was also observed by TEM, which revealed loss of eletrondensity and organelles structure alteration. These results suggest internal and external activities on the yeast cells and the potential use of FCB against M. pachydermatis.

 

KEY WORDS: Bacterial metabolites, Burkholderia sp., growing inhibition, Malassezia pachydermatis.

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Published

2008-07-09

How to Cite

VIEIRA-DA-MOTTA, Olney; SILVA, Maria José Ribeiro da; DEUS, Mariana Feitosa de; SAMUELS, Richard Ian; MATHIAS, Luciana; ALMEIDA, João Carlos de Aquino; GUIMARÃES, Erick Vaz; MORAES-NETO, Antonio Henrique Almeida. IN VITRO ACTIVITY OF SECONDARY METABOLITES OF Burkholderia sp. (BURKHOLDER 1950) AGAINST THE YEAST Malassezia pachydermatis (WEIDMANN) DODGE 1935. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 9, n. 2, p. 410–417, 2008. Disponível em: https://revistas.ufg.br/vet/article/view/1040. Acesso em: 28 dec. 2025.

Issue

Vol. 9 No. 2 (2008)

Section

Veterinary Medicine

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Copyright (c) 2008 Brazilian Animal Science/ Ciência Animal Brasileira

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