INFLUENCE OF REFRIGERATION ON THE PRESERVATION OF THE TOTAL NUMBER OF HEMOCYTES OF SEA SHRIMPS Litopenaeus vannamei CULTIVATED USING SODIUM CITRATE

Authors

  • Andrea C. Gomes Barretto UFRPE
  • Lilian M.N. de Barros Góes
  • Dulcilene Lacerda do Nascimento
  • Paulo de Paula Mendes UFRPE
  • Eneida Wilcox Rêgo
  • Emiko Shinozaki Mendes

DOI:

https://doi.org/10.5216/cab.v13i4.5647

Keywords:

Aquaculture

Abstract

For carrying out blood cell count, the use anti-coagulants is necessary, and in the specific case of the hemolymph  in shrimp, there is still no standard solution to avoid its coagulation. Sodium citrate is frequently used as anti-coagulant, because it has been previously proven to be efficient in the preservation of the hemocytes of the sea shrimp Litopenaeus vannamei. In 140 samples of hemolymph with sodium citrate (10%) in the ratio of 1:1, collected at 2 stages of cultivation, in dry and wet season, were examined for HTC immediately after collection and then again after storage in the refrigerator. All data were analyzed using modeling mathematics formulas (P < 0.05). After storage in the refrigerator, the number of cells decreased considerably. Therefore, the anticoagulant sodium citrate only preserved the hemolymph of the shrimp
KEYWORDS: anticoagulant; haemolynph; shrimp.

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Author Biography

Andrea C. Gomes Barretto, UFRPE

Departamento de Medicina Veterinária

Laboratório de sanidade dos animais aquáticos

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Published

2012-12-14

How to Cite

BARRETTO, A. C. G.; GÓES, L. M. de B.; NASCIMENTO, D. L. do; MENDES, P. de P.; RÊGO, E. W.; MENDES, E. S. INFLUENCE OF REFRIGERATION ON THE PRESERVATION OF THE TOTAL NUMBER OF HEMOCYTES OF SEA SHRIMPS Litopenaeus vannamei CULTIVATED USING SODIUM CITRATE. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 13, n. 4, p. 520–524, 2012. DOI: 10.5216/cab.v13i4.5647. Disponível em: https://revistas.ufg.br/vet/article/view/5647. Acesso em: 11 may. 2024.

Issue

Vol. 13 No. 4 (2012)

Section

Veterinary Medicine

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