ANTIBODIES DETECTION TO RHODOCOCCUS EQUI IN VACCINATED MARES AND FOALS BY INDIRECT ENZYME IMMUNOASSAY

Authors

  • Carla Braga Martins Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil
  • Marco Augusto Giannoccaro da Silva Universidade Federal do Tocantins (UFT), Araguaína, Tocantins, Brasil
  • Claudia Acosta Duarte Universidade Estadual do Norte do Paraná (UENP), Bandeirantes, Paraná, Brasil
  • Raquel Mincarelli Albernaz Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil
  • José Correa de Lacerda Neto Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil
  • Rosangela Zacarias Machado Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil

DOI:

https://doi.org/10.5216/cab.v11i1.5207

Keywords:

Foals, antibodies, ELISA, Rhodococcus equi, immunization

Abstract

The humoral immune response in ‘Brasileiro de Hipismo’ (BH) breed and Breton mares was compared after using the Rhodococcus equi vaccine, and the effect of maternal immunoprophylaxis on antibody transfer to newborn foals through the colostrum was evaluated. Blood samples were obtained from 16 pregnant mares vaccinated against R. equi, 16 foals (offspring from vaccinated mares), 8 unvaccinated pregnant mares and 8 foals (offspring from control mares). R equi serum antibody titers were determined by enzyme-linked immunosorbant assay (ELISA) after the immunization of pregnant mares using two different antigens, APTX and the commercial vaccine. There was no difference in antibody production between the two breeds. Significant increase in R. equi antibody titers was observed in mares after vaccination (p<0.01), reaching a peak at foaling. Afterward, titers tended to decrease for up to 60 days after birth (dab) and then remained constant until 150 dab. Significant antibody transfer to the vaccinated mares newborn foal occurred through the colostrum. A slight reduction in antibody titer was observed at 60 dab, after which titers remained constant for up to 150 dab. The commercial antigen detected significantly higher antibody titers than did APTX (p<0.01).

KEY WORDS: Antibodies, ELISA, Rhodococcus equi, immunization, foals.

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Published

2010-04-01

How to Cite

MARTINS, Carla Braga; SILVA, Marco Augusto Giannoccaro da; DUARTE, Claudia Acosta; ALBERNAZ, Raquel Mincarelli; NETO, José Correa de Lacerda; MACHADO, Rosangela Zacarias. ANTIBODIES DETECTION TO RHODOCOCCUS EQUI IN VACCINATED MARES AND FOALS BY INDIRECT ENZYME IMMUNOASSAY. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 11, n. 1, p. 194–200, 2010. DOI: 10.5216/cab.v11i1.5207. Disponível em: https://revistas.ufg.br/vet/article/view/5207. Acesso em: 17 dec. 2025.

Issue

Vol. 11 No. 1 (2010)

Section

Veterinary Medicine

License

Copyright (c) 2010 Brazilian Animal Science/ Ciência Animal Brasileira

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