EFECT OF MINIMUM CONTAMINATION MEDIUM (MMC) AND MODIFIED EGG YOLK LACTOSE (LGM) EXTENDERS ON CANINE SEMEN VIABILITY AFTER COOLING AT 4 ºC FOR 24 HOURS IN EQUITAINER®

Authors

  • Bianca Perez Barcellos UFF
  • Marco Antônio Rocha UFF
  • Luciano Jamas Polícia Militar do Rio de Janeiro
  • Felipe Zandonadi Brandão UFF
  • Luis Garcia Nogueira UFF
  • Tania Goes de Pinho UFF

DOI:

https://doi.org/10.5216/cab.v12i1.4692

Keywords:

Animal reproduction

Abstract

The aim of this study was to compare the viability of canine semen after dilution in Minimum Contamination Medium (MMC) and Modified Lactose-Egg yolk extender (LGM) and incubation at 4 ºC for 12 and 24 hours. Thirteen ejaculates were collected from 5 adult dogs by digital manipulation. Macroscopic and microscopic characteristics were assessed right after collection. Semen was divided to be diluted (1:3) in MMC and LGM and subsequently chilled in Equitainer®. Seminal parameters of motility and spermatic morphology, membrane integrity (hyposmotic test), spermatic viability and spontaneous acrosome reaction (Trypan-blue Giemsa stain) were evaluated in fresh and chilled semen after 12 and 24 hours of incubation at 4 ºC.  No difference between extenders was identified in semen conservation after 12 hours. After 24 hours just spermatic motility was different (p>0.05). But, after both periods of conservation, semen diluted in LGM maintained most of the characteristics verified in the fresh semen. The mean of true and false acrosome reaction did not exceed 2% in both semen extenders, which demonstrates no influence of media component and incubation period on these phenomena at 4 ºC. In conclusion, these results indicate that both extenders can be used in canine semen conservation at 4 ºC in contêiner during 12 hours without significant changes in semen characteristics.

KEYWORDS: Dogs, sperm, cooling.

Downloads

Download data is not yet available.

Author Biography

Tania Goes de Pinho, UFF

Departamento de patologia e clínica veterinária. Fisiopatologia da reprodução.

Downloads

Published

2011-03-31

How to Cite

BARCELLOS, B. P.; ROCHA, M. A.; JAMAS, L.; BRANDÃO, F. Z.; NOGUEIRA, L. G.; PINHO, T. G. de. EFECT OF MINIMUM CONTAMINATION MEDIUM (MMC) AND MODIFIED EGG YOLK LACTOSE (LGM) EXTENDERS ON CANINE SEMEN VIABILITY AFTER COOLING AT 4 ºC FOR 24 HOURS IN EQUITAINER®. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 12, n. 1, p. 126–133, 2011. DOI: 10.5216/cab.v12i1.4692. Disponível em: https://revistas.ufg.br/vet/article/view/4692. Acesso em: 30 jun. 2024.

Issue

Vol. 12 No. 1 (2011)

Section

Veterinary Medicine

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).