SENSIBILITY OF MICROCULTIVATION ASSAY DERMATOBAC® FOR FUNGAL ENDOMETRITIS DIAGNOSIS IN MARES

Authors

  • Eriky Akio de Oliveira Tongu Universidade de Santo Amaro - UNISA
  • Carlos Peleschi Taborda Departamento de Ciências Biomédicas, Universidade de São Paulo - USP
  • Shirlei Aparecida Vieira Marques Departamento de Ciências Biomédicas, Universidade de São Paulo - USP
  • Gustavo Mendes Gomes Universidade Severino Sombra - USS
  • Letícia Patrão de Macedo Gomes Universidade Severino Sombra - USS
  • Kleber da Cunha Peixoto Jr. Universidade de Santo Amaro - UNISA
  • André Maciel Crespilho Universidade de Santo Amaro- UNISA Laboratório de Reprodução Animal

DOI:

https://doi.org/10.1590/cab.v16i4.31444

Keywords:

Animal Reproduction

Abstract

Among different etiologic factors, fungal endometritis is a common cause of decreased mare’s fertility.Two experiments were conducted to evaluate the sensitivity of Dermatobac®, a microculture system originally developed for the isolation of fungi that concerns Human Medicine. In Experiment 1, standard(ATCC) strains of Candida (n=4) and Aspergyllus (n=1) were grown and visually analyzed daily for 7days. For Experiment 2, 34 mares were selected and subjected to material harvesting for completionof uterine cytology as well as seeding with Dermatobac®. After 24h of cultivation, the macroscopicobservation of all Candida and Aspergyllus strains (Experiment 1) was possible. In Experiment 2,five animals showed presence of yeast on cytologic slides (14.71%), results also confirmed sowingin Dermatobac® system (100% sensitivity). Five false positive cases were observed, characterizedby microbiological growth in Dermatobac® but not confirmed by endometrial cytology, determiningspecificity of 85%. However, the results did not compromise the efficiency of Dermatobac® systemgiven all contaminants presented differentiated morphology and higher growing time (P<0.05)when compared to the major etiological agents of equine fungal endometritis. The Dermatobac®system proved to be efficient for the macroscopic diagnosis of fungal endometritis in horses, showingconclusive information only after 24 hours of culture.

Keywords: Dermatobac®; diagnosis; endometritis; fungi; mare.

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Published

2015-10-30

How to Cite

TONGU, E. A. de O.; TABORDA, C. P.; MARQUES, S. A. V.; GOMES, G. M.; GOMES, L. P. de M.; PEIXOTO JR., K. da C.; CRESPILHO, A. M. SENSIBILITY OF MICROCULTIVATION ASSAY DERMATOBAC® FOR FUNGAL ENDOMETRITIS DIAGNOSIS IN MARES. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 16, n. 4, p. 598–607, 2015. DOI: 10.1590/cab.v16i4.31444. Disponível em: https://revistas.ufg.br/vet/article/view/31444. Acesso em: 23 may. 2024.

Issue

Vol. 16 No. 4 (2015)

Section

Veterinary Medicine

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