Morphologic evaluation of goat spermatozoa diluted and frozen in media based on powder coconut water (PCW-101) or TRIS, stained by eosin-nigrosin and bromophenol blue
DOI:
https://doi.org/10.5216/cab.v10i3.2278Keywords:
Animal reproduction clinicalAbstract
This aims of the work were: to evaluate in vitro the goat semen frozen in diluents media based on powder coconut water (PCW-101) and TRIS and, compare the bromophenol blue stain efficiency with the eosin-nigrosin stain. The ejaculateds were divided and diluted into PCW-101 and TRIS, frozed and thawed after 30 days. Spermatic morphology was evaluated, through semen smears stained by eosin-nigrosin (EN) and bromophenol blue (BB). The morphologic parameters evaluated were: normal spermatozoa (N), head alteration (HA), intermediary piece alteration (IPA), tail alteration (TA), proximal citoplasmic drop (PCD), distal citoplasmic drop (DCD), and detached head (DH). There wasn’t significant difference in the observation of N between media, staining and their interactions after 5 minutes of thermo resistance test. After 120, the N was significantly influenced by media, where the TRIS presented better results. The incubation period of 120 minutes at 37ºC affect the spermatic morphology, increasing the HA percentages. The media based on TRIS promoted better protection from the cryoinjuries on frozen goat spermatozoa. BB staining was efficient on the fresh and post-thaw goat semen evaluation.
KEY WORDS: Bromophenol blue, goat, powder coconut water; TRIS, spermatozoa.
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