EVALUATION OF BLOOD ON FILTER PAPER TO DETECTION AND QUANTIFICATION OF THE ANTIBO¬DIES TO NEWCASTLE DISEASE VIRUS

Authors

  • Fabiane Fonseca
  • Silvia de Oliveira Hübner
  • Gilberto D’Avila Vargas
  • Geferson Fischer
  • Telmo Vidor

DOI:

https://doi.org/10.5216/cab.v8i2.1357

Abstract

The present study describes the evaluation of a filter paper blood sampling technique for assessment of the level of antibodies against the Newcastle disease virus (NDV) by hemagglutination inhibition (HI) test. Blood samples were collected from ninety eigth chickens with a known history of vaccination against NDV and analyzed by HI method. The blood was impregnated onto Whatman no. 1 filter paper with 3cm2 and also collected in tubes for obtaining serum. The papers were stored at room temperature and tested within fourty five days. The resulting sensitivity, specificity, positive predictive value and negative predictive value of the filter paper HI compared to serum HI was 89%, 73%, 76% and 87%, respectively. It was found index precision of the 80% and the correlation coefficient r = 0.81%. The overall average geometrical titer of antibodies was 33.1 and 33.6 in eluates and in sera, respectively. Association between the two methods was found to be highly significant (p < 0.001) when analyzed by Fishers test. The results indicate the potential use of filter paper blood sampling technique as an alternative to serum for detection of the antibodies against NDV. KEY-WORDS: Chicken, hemagglutination inhibition, serology.

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Published

2007-07-05

How to Cite

FONSECA, F.; HÜBNER, S. de O.; VARGAS, G. D.; FISCHER, G.; VIDOR, T. EVALUATION OF BLOOD ON FILTER PAPER TO DETECTION AND QUANTIFICATION OF THE ANTIBO¬DIES TO NEWCASTLE DISEASE VIRUS. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 8, n. 2, p. 319–324, 2007. DOI: 10.5216/cab.v8i2.1357. Disponível em: https://revistas.ufg.br/vet/article/view/1357. Acesso em: 23 nov. 2024.

Issue

Vol. 8 No. 2 (2007)

Section

Veterinary Medicine

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