EVALUATION OF THE GROWTH FACTOR IGF-I IN THE IN VITRO MATURATION OF CANINE OOCYTES IN THE TCM 199 MEDIUM

Authors

  • Kellen de Sousa Oliveira Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil
  • Marco Antônio Machado Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil
  • Ana Paula Coelho Ribeiro Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil
  • Maricy Apparício Ferreira Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil
  • Gilson Hélio Toniollo Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil

Keywords:

Sanidade Animal

Abstract

The aim was to evaluate the supplementation of recombinant IGF-I growth factor at concentration of 100?g/mL in 500µL of TCM 199 medium in the in vitro maturation of canine oocytes. The oocytes were stained with isotiocianate of fluorescein (Lens cullinaris) and Hoeschst 33342 for nuclear and cytoplasmatic assessment using an epifluorescence microscope (filter FITC: excitement 490nm and emission 520nm). There were no statistical differences between control and supplemented groups regarding cytoplasmatic and nuclear maturation when using Kruskal-Wallis test, p<0,05.

Key words: Canine, IGF-I, maturation, oocyte.

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Published

2009-07-03

How to Cite

OLIVEIRA, Kellen de Sousa; MACHADO, Marco Antônio; RIBEIRO, Ana Paula Coelho; FERREIRA, Maricy Apparício; TONIOLLO, Gilson Hélio. EVALUATION OF THE GROWTH FACTOR IGF-I IN THE IN VITRO MATURATION OF CANINE OOCYTES IN THE TCM 199 MEDIUM. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 10, n. 2, p. 560–567, 2009. Disponível em: https://revistas.ufg.br/vet/article/view/1121. Acesso em: 20 dec. 2025.

Issue

Vol. 10 No. 2 (2009)

Section

Veterinary Medicine

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Copyright (c) 2009 Brazilian Animal Science/ Ciência Animal Brasileira

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