EVALUATION OF THE GROWTH FACTOR IGF-I IN THE IN VITRO MATURATION OF CANINE OOCYTES IN THE TCM 199 MEDIUM

Authors

  • Kellen de Sousa, Oliveira Faculdade de ciencias agrárias e veterinárias- UNESP
  • Marco Antônio Machado Faculdade de ciencias agrárias e veterinárias- UNESP
  • Ana Paula Coelho Ribeiro Faculdade de ciencias agrárias e veterinárias- UNESP
  • Maricy Apparício Ferreira Faculdade de ciencias agrárias e veterinárias- UNESP
  • Gilson Hélio Toniollo Faculdade de ciencias agrárias e veterinárias- UNESP

DOI:

https://doi.org/10.5216/cab.v10i2.1121

Keywords:

Sanidade Animal

Abstract

The aim was to evaluate the supplementation of recombinant IGF-I growth factor at concentration of 100?g/mL in 500µL of TCM 199 medium in the in vitro maturation of canine oocytes. The oocytes were stained with isotiocianate of fluorescein (Lens cullinaris) and Hoeschst 33342 for nuclear and cytoplasmatic assessment using an epifluorescence microscope (filter FITC: excitement 490nm and emission 520nm). There were no statistical differences between control and supplemented groups regarding cytoplasmatic and nuclear maturation when using Kruskal-Wallis test, p<0,05.

Key words: Canine, IGF-I, maturation, oocyte.

Downloads

Download data is not yet available.

Author Biographies

Kellen de Sousa, Oliveira, Faculdade de ciencias agrárias e veterinárias- UNESP

1.Pós-graduando (a) em Med. Veterinária – FCAV/UNESP, ksoliver13@hotmail.com

Marco Antônio Machado, Faculdade de ciencias agrárias e veterinárias- UNESP

1.Pós-graduando (a) em Med. Veterinária – FCAV/UNESP,

Ana Paula Coelho Ribeiro, Faculdade de ciencias agrárias e veterinárias- UNESP

2.Pós-graduanda em Cirurgia Veterinária – FCAV/UNESP

Maricy Apparício Ferreira, Faculdade de ciencias agrárias e veterinárias- UNESP

2.Pós-graduanda em Cirurgia Veterinária – FCAV/UNESP

Gilson Hélio Toniollo, Faculdade de ciencias agrárias e veterinárias- UNESP

3.Prof. Titular do Dep. de Med. Vet. e Rep. Animal – FCAV/UNESP, toniollo@fcav.unesp.br

Downloads

Published

2009-07-03

How to Cite

OLIVEIRA, K. de S.; MACHADO, M. A.; RIBEIRO, A. P. C.; FERREIRA, M. A.; TONIOLLO, G. H. EVALUATION OF THE GROWTH FACTOR IGF-I IN THE IN VITRO MATURATION OF CANINE OOCYTES IN THE TCM 199 MEDIUM. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 10, n. 2, p. 560–567, 2009. DOI: 10.5216/cab.v10i2.1121. Disponível em: https://revistas.ufg.br/vet/article/view/1121. Acesso em: 30 jun. 2024.

Issue

Vol. 10 No. 2 (2009)

Section

Veterinary Medicine

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).