OBTENTION METHODS OF RESPIRATORY TRACT CELSS IN HEALTHY SHEEP BY THE TRACHEOBRONCHEAL LAVAGE TECHNIQUE BY NASOTRACHEAL VIA

Authors

  • Julio Simões Marcondes Faculdade de Medicina Veterinária e Zootecnia - Unesp Botucatu
  • Roberto Calderon Gonçalves Faculdade de Medicina Veterinária e Zootecnia - Unesp Botucatu

DOI:

https://doi.org/10.5216/cab.v9i2.1049

Keywords:

Sanidade Animal, ovinos, lavado traqueobrônquico, citologia

Abstract

The technique of pulmonary cells obtention by nasotracheal intubation was evaluated and the cellular population of 19 healthy sheep was characterized. The animals were hold standing still with head and neck strait; aligned with the vertebral spine and a silicone tube guide till the carina, to introduce a lower caliber tube for precede the tracheobronchial lavage. The average counting of nucleated cells was 64.650 ± 49.674. The cytological analyses of the samples slowed by the average of obtained percentages: 62.74% of macrophages, 15.01% cylindrical epithelial cells, 20.04% of neutrophills, 1.44 of lymphocytes and 0.77% of eosinophills. It was concluded that this methods of sample obtention by nasotracheal intubation to reach the tracheobronchial region was efficient to cytological characterization and cellular differentiation in the obtained samples, being well supported by the animals.

 

KEY WORDS: Citocentrifugation, cytology, tracheobronchial lavage, sheep.

Downloads

Download data is not yet available.

Author Biographies

Julio Simões Marcondes, Faculdade de Medicina Veterinária e Zootecnia - Unesp Botucatu

Pós graduando da Área de Clínica de Grandes Animais da Faculdade de Medicina Veterinária e Zootecnia – FMVZ, Botucatu – Universidade Estadual Paulista. Departamento de Clínica Veterinária

Roberto Calderon Gonçalves, Faculdade de Medicina Veterinária e Zootecnia - Unesp Botucatu

Prof° Ass. Dr. da área de Clínica de Grandes Animais da Faculdade de Medicina Veterinária e Zootecnia – FMVZ, Botucatu – Universidade Estadual Paulista. Departamento de Clínica Veterinária.

Downloads

Published

2008-07-09

How to Cite

MARCONDES, J. S.; GONÇALVES, R. C. OBTENTION METHODS OF RESPIRATORY TRACT CELSS IN HEALTHY SHEEP BY THE TRACHEOBRONCHEAL LAVAGE TECHNIQUE BY NASOTRACHEAL VIA. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 9, n. 2, p. 418–426, 2008. DOI: 10.5216/cab.v9i2.1049. Disponível em: https://revistas.ufg.br/vet/article/view/1049. Acesso em: 12 may. 2024.

Issue

Vol. 9 No. 2 (2008)

Section

Veterinary Medicine

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).