IN VITRO ACTIVITY OF SECONDARY METABOLITES OF Burkholderia sp. (BURKHOLDER 1950) AGAINST THE YEAST Malassezia pachydermatis (WEIDMANN) DODGE 1935.

Authors

  • Olney Vieira-da-Motta Universidade Estadual do Norte Fluminense Darcy Ribeiro
  • Maria José Ribeiro da Silva Universidade Estadual do Norte Fluminense Darcy Ribeiro
  • Mariana Feitosa de Deus Universidade Estadual do Norte Fluminense Darcy Ribeiro
  • Richard Ian Samuels Universidade Estadual do Norte Fluminense Darcy Ribeiro
  • Luciana Mathias Universidade Estadual do Norte Fluminense Darcy Ribeiro
  • João Carlos de Aquino Almeida Universidade Estadual do Norte Fluminense Darcy Ribeiro
  • Erick Vaz Guimarães Fiocruz
  • Antonio Henrique Almeida Moraes-Neto FIOCRUZ

DOI:

https://doi.org/10.5216/cab.v9i2.1040

Keywords:

Sanidade Animal

Abstract

M. pachydermatis is among the main agents involved in skin lesion and otopathological alterations of dogs and cats frequently associated to bacterial infections and host immune suppression. Because of resistance toward drugs, the market urge new therapies for treatment of fungal infections are needed. The effect of metabolites present in the filtrate culture of Burkholderia sp. (FCB) was tested against native clinical strains of the yeast M. pachydermatis in liquid medium measured by standard growth curve, by agar diffusion method, and colony forming units count in agar. Activity of FCB on cells was evaluated by transmission eletron microscopy (TEM). For comparison, sensitivity tests were performed on the yeast by diffusion agar test using commercial drugs. Crude FCB presented activity against the yeast by the methods utilized. The activity of FCB on cells was also observed by TEM, which revealed loss of eletrondensity and organelles structure alteration. These results suggest internal and external activities on the yeast cells and the potential use of FCB against M. pachydermatis.

 

KEY WORDS: Bacterial metabolites, Burkholderia sp., growing inhibition, Malassezia pachydermatis.

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Author Biographies

Olney Vieira-da-Motta, Universidade Estadual do Norte Fluminense Darcy Ribeiro

Professor Associado II do Curso de Medicina Veterinária da UENF, do Centro de Ciências e Tecnologias Agropecuárias, Laboratório de Sanidade Animal

Maria José Ribeiro da Silva, Universidade Estadual do Norte Fluminense Darcy Ribeiro

Médica Veterinária, formada na UENF em 2003; mestranda em Produção Animal pelo Laboratório de Sanidade Animal/CCTA

Mariana Feitosa de Deus, Universidade Estadual do Norte Fluminense Darcy Ribeiro

Bióloga, formada na UENF em 2003, mestranda do Laboratório de Entomologia e Fitopatologia, CCTA.

Richard Ian Samuels, Universidade Estadual do Norte Fluminense Darcy Ribeiro

Biólogo formado pela Universidade de Bath, no Reino Unido, professor do curso de Agronomia, membro do Laboratório de Entomologia e Fitopatologia, CCTA.

Luciana Mathias, Universidade Estadual do Norte Fluminense Darcy Ribeiro

Graduanda em Biologia, bolsista de Iniciação Científica do Laboratório de Fisiologia e Bioquímica de Microrganismos, Centro de Biociências e Biotecnologias/CBB.

João Carlos de Aquino Almeida, Universidade Estadual do Norte Fluminense Darcy Ribeiro

Biólogo, doutorado em Ciências Biológicas UFRJ, professor do Laboratório de Fisiologia e Bioquímica de Microrganismos, CBB.

Erick Vaz Guimarães, Fiocruz

Mestre em Biologia Celular e Molecular, formado pela Fiocruz, doutorando do Departamento de Ultra Estrutura e Biologia Celular-Fiocruz

Antonio Henrique Almeida Moraes-Neto, FIOCRUZ

Doutor em Ciências Biológicas, UFRJ, pesquisadorLaboratório de Ecoepidemiologia e Controle da Esquistossomose e Geohelmintoses, Departamento de Biologia-Fiocruz

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Published

2008-07-09

How to Cite

VIEIRA-DA-MOTTA, O.; SILVA, M. J. R. da; DEUS, M. F. de; SAMUELS, R. I.; MATHIAS, L.; ALMEIDA, J. C. de A.; GUIMARÃES, E. V.; MORAES-NETO, A. H. A. IN VITRO ACTIVITY OF SECONDARY METABOLITES OF Burkholderia sp. (BURKHOLDER 1950) AGAINST THE YEAST Malassezia pachydermatis (WEIDMANN) DODGE 1935. Brazilian Animal Science/ Ciência Animal Brasileira, Goiânia, v. 9, n. 2, p. 410–417, 2008. DOI: 10.5216/cab.v9i2.1040. Disponível em: https://revistas.ufg.br/vet/article/view/1040. Acesso em: 30 jun. 2024.

Issue

Vol. 9 No. 2 (2008)

Section

Veterinary Medicine

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