Implante 3D de copoliamida associada à elastômero termoplástico  (PCTPE) para reparação traqueal de coelhos (Oryctolagus cuniculus):  estudo preliminar

Ariadne Rein; Marcelo Carrijo da Costa; Gabriel Montanhin; Gustavo Fernandes; Marcella Dall’ Agnol Leite; Thiago André Salvitti de Sá Rocha; Gabriel João Unger Carra; Rosemeri de Oliveira Vasconcelos; Luís Gustavo Gosuen Gonçalves Dias; Paola Castro Moraes

doi:10.1590/1809-6891v25e-76225E

Autores

Ariadne Rein Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil https://orcid.org/0000-0003-0181-6768
Marcelo Carrijo da Costa Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil https://orcid.org/0000-0003-4014-4351
Gabriel Montanhin Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil https://orcid.org/0000-0002-9830-041X
Gustavo Fernandes Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil https://orcid.org/0000-0001-5410-476X
Marcella Dall’ Agnol Leite Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil https://orcid.org/0000-0001-8930-6791
Thiago André Salvitti de Sá Rocha Universidade Federal de Jataí - GO https://orcid.org/0000-0003-1414-3101
Gabriel João Unger Carra Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil https://orcid.org/0000-0003-1355-1955
Rosemeri de Oliveira Vasconcelos Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil https://orcid.org/0000-0003-2319-5309
Luís Gustavo Gosuen Gonçalves Dias Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil https://orcid.org/0000-0003-3993-1649
Paola Castro Moraes Universidade Estadual Paulista (UNESP), Jaboticabal, São Paulo, Brasil https://orcid.org/0000-0002-0255-2971

DOI:

https://doi.org/10.1590/1809-6891v25e-76225E

Resumo

Defeitos de grandes segmentos traqueais podem apresentar sério problema clínico, devido à ausência de substitutos na cirurgia reconstrutiva. Os biomateriais poliméricos estão entre os mais utilizados na medicina. Entretanto, a implantação de um biomaterial desencadeia uma série de eventos biológicos e a biocompatibilidade do material é de extrema importância para a medicina regenerativa. Objetivou-se com este estudo avaliar o uso da copoliamida associada ao elastômero termoplástico (PCTPE) em próteses impressas em 3D para reparação de defeitos parciais da traqueia de coelhos. Foram utilizados 16 coelhos machos da raça Nova Zelândia Branco, submetidos à criação de um defeito parcial de traqueia na região ventral do 4º ao 8º anel traqueal. Os animais foram subdivididos (n=4) de acordo com o momento das eutanásias, programadas com intervalo de sete (G7), quinze (G15), trinta (G30) e sessenta dias (G60). A análise histopatológica por hematoxilina e eosina (HE) revelou que o implante 3D de PCTPE apresentou reação do tipo corpo estranho e a inflamação persistiu até os 60 dias de avaliação. A histoquímica de picrosirius vermelho revelou tendência de maior quantidade de colágeno tipo I nos tempos iniciais de inflamação, enquanto o colágeno tipo III foi predominante nos períodos tardios de avaliação. Pode- se sugerir reação exacerbada e não adequada da interface tecido-material e perpetuação do processo inflamatório.

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