Ex vivo evaluation of anterior lens capsule staining in horses with three concentrations of gentian violet for surgical training

Marcelle Bettio; Maiara Poersch Seibel; Maria Eduarda Mattos Franceschini; Rafaella Silva Rocha; Renata Lima Baptista; Anita Marchionatti Pigatto; João Antonio Tadeu Pigatto

doi:10.1590/1809-6891v24e-76196E

Autores

Marcelle Bettio Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0009-0008-3798-6682
Maiara Poersch Seibel Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-5295-4979
Maria Eduarda Mattos Franceschini Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-6705-2740
Rafaella Silva Rocha Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0001-8856-8239
Renata Lima Baptista Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-7138-9020
Anita Marchionatti Pigatto Universidade Estadual Paulista "Júlio de Mesquita Filho (UNESP), Botucatu, São Paulo, Brasil https://orcid.org/0000-0002-0521-7984
João Antonio Tadeu Pigatto Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-1541-9133

DOI:

https://doi.org/10.1590/1809-6891v24e-76196E

Resumo

O objetivo deste estudo foi avaliar e comparar a eficácia de três concentrações de violeta genciana (0,5%, 0,1% e 0,05%) na coloração da cápsula anterior da lente em equinos. Trinta e seis olhos de equinos post-morten foram utilizados. De acordo com a concentração de violeta genciana utilizada, os olhos foram subdivididos em três grupos compostos por 12 olhos cada. A avaliação da eficácia em coloração da cápsula anterior da lente com diferentes concentrações de violeta de genciana foi realizada por meio de um sistema empírico de avaliação da coloração adequada ou inadequada de retalhos capsulares. Com base na avaliação dos examinadores, as concentrações de 0,1% e 0,05% de violeta de genciana permitiram a visualização adequada da cápsula anterior para o treinamento da capsulotomia curvilínea contínua enquanto a concentração de 0,5% produziu uma coloração capsular forte e inadequada. O modelo desenvolvido com violeta genciana, nas concentrações de 0,1% e 0,05%, permitiu a visualização nítida do retalho capsular, o que o torna viável como modelo para treinamento da etapa de capsulotomia curvilínea contínua em cirurgia de catarata em equinos.
Palavras-chave: capsulotomia anterior; corantes vitais; equino; laboratório úmido; treinamento cirúrgico

Downloads

Não há dados estatísticos.

Referências

McMullen Junior RJ, Utter ME. Current developments in equine cataract surgery. Equine Veterinary Journal. Supplement. 2010;37:38-45. Available from: doi:10.1111/j.2042-3306.2010.tb05633.x.

Jhanju V, Chan E, Das S, Zhang H, Vajpayee RB. Trypan blue dye for anterior segment surgeries . Eye (Lond) 2011;25(9):1113-1120. Available from: doi:10.1038/eye.2011.139.

MCMULLEN RJ, Stoppini R. Diseases and surgery of the lens. In: Gilger BC, editor. Equine Ophthalmology. 4th ed. Hoboken : John Wiley & Sons; 2017. p.416-452. Available from: doi:10.1002/9781119782285.ch7.

Jacobs DS, Cox TA, Wagoner MD, Ariyasu RG, Karp CL. Capsule staining as an adjunct to cataract surgery. Ophthalmology. 2006;113(4):707-713. Available from: doi:10.1016/j.ophtha.2006.01.024.

Hassaballa MAM, Osman AAEL. Delineating the extent of anterior capsulorhexis with gentian violet using capsulorhexis marker: a preliminary study of efficacy and toxicity in an animal model. Clinical Ophthalmology. 2011;5:831-836. Available from: doi:10.2147/OPTH.S21791.

Haeussler-Sinangin Y, Dahlhoff D, Schultz T, Dick HB. Clinical performance in continuous curvilinear capsulorhexis creation supported by a digital image guidance system. Journal of Cataract and Refractive Surgery. 2017;43(3):348-352. Available from: doi:10.1016/j.jcrs.2016.12.027.

Hu WF, Chen SH. Advances in capsulorhexis. Current Opinion Ophthalmology. 2019;30(1):19-24. Available from: doi: 10.1097/ICU.0000000000000539.

Sharma B, Abell RG, Arora T, Antony T, Vajpayee RB. Techniques of anterior capsulotomy in cataract surgery. Indian Journal of Ophthalmology. 2019;67(4):450-460. Available from: doi: 10.4103/ijo.IJO_1728_18.

Eldin SAG, Mehelmy EM, Shazli EM, Mostafa YM. Experimental staining of the anterior lens capsule in albino rabbits. Journal of Cataract and Refractive Surgery. 1999;25(9):1289-1294. Available from: doi: 10.1016/s0886-3350(99)00153-4.

Melles GRJ, Waard PW, Pameyer JH, Beekhuis WH. Trypan blue capsule staining I'm visualizethe capsulorhexis in cataract surgery. Journal of Cataract Refractive Surgery. 1999;25(1):7-9. Available from: doi:10.1016/s0886-3350(99)80004-2.

Rodrigues EB, Costa EF, Penha FM, Melo GB, Bottós J, Dib E, et al. The use of vital dyes in ocular surgery. Survey of Ophthalmology. 2009;54(5):576-617. Available from: doi: 10.1016/j.survophthal.2009.04.011.

Andjelić S, Zupančič G, Hawlina M. The effect of gentian violet on human previous lens epithelial cells. Current Eye Research. 2014;39(10):1020-1025. Available from: doi: 10.3109/02713683.2014.894077.

Simsek C, Gokmen O. The effects of vital dyes on mechanic properties of the human previous lens capsule. Indian Journal of Ophthalmology. 2020;68:66-70. Available from: doi: 10.4103/ijo.IJO_285_19.

Wilińska, J, Mocanu, B, Awad, D, Gousia, D, Hillner, C, Brannath, W, et al. New stains for anterior capsule surgery. Journal of Cataract and Refractive Surgery. 2019;45(2): 213–218. Available from: 10.1016/j.jcrs.2018.09.016.

Dada VK, Sharma MDM, Sudan R, Sethi H, Dada T, Pangtey MS, et al. Anterior capsule staining for capsulorhexis in cases of white cataract. Journal of Cataract and Refractive Surgery. 2004;30(2):326-333. Available from: doi: 10.1016/S0886-3350(03)00573-X.

Prakash G, Jhanji V, Sharma N, Gupta K, Titiyal JS, Vajpayee RB. Assessment of perceived difficulties by residents in performing routine steps in phacoemulsification surgery and in managing complications. Canadian Journal of Ophthalmology. 2009;44(3):284-287. Available from: doi: 10.3129/i09-051.

Oflaz AB, Köktekir, BE, Okudan S. Does cataract surgery simulation correlate with real- life experience? Turkish Journal of Ophthalmology. 2018;48(3):122-6. Available from: doi: 10.4274/tjo.10586.

Dong J, Yang X, Yang X, Li J. A practical continuous curvilinear capsulorhexis self-training system. Indian Journal of Ophthalmology. 2021;69(10):2678-286. Available from: doi: 10.4103/ijo.IJO_210_21.

Pujari A, Saluja G, Bhaskaran K, Modaboyina S, Asif MI, Agarwal T, et al. Animal and corpse human eyes for residents' surgical training in ophthalmology. Survey Ophthalmology. 2021;67(1):226-251. Available from: doi: 10.1016/j.survophthal.2021.05.004.

Chang YS, Tseng S, Tseng S. Comparison of dyes for cataract surgery: Part 2: Effectiveness of capsule staining in a rabbit model. Journal of Cataract and Refractive Surgery. 2005;31(4):799-804. Available from: doi: 10.1016/j.jcrs.2004.09.029.

Hisatomi T, Enaida H, Matsumoto H, Kagimoto T, Ueno A, Hata Y, et al. staining ability and biocompatibility of brilliant blue G. Archives of Ophthalmology. 2006;124(4):514-9. Available from: doi:10.1001/archopht.124.4.514.

Landis JR, Koch GG. The measurement of observer agreement for categorical data. Biometrics. 1977;33:159-74 . Available from: https://doi.org/10.2307/2529310

Kirkwood BR, Sterne JAC. Essential medical statistics. 2nd ed. Massachusetts: Blackwell Science; 2006. Available from: https://bcs.wiley.com/he-bcs/Books?action=index&bcsId=11848&itemId=0865428719

Pona A, Quan, EY, Cline A, Feldman SR. Review of the use of gentian violet in dermatology practice. Dermatology Online Journal. 2020;26(5):13030. Available from: https://doi.org/10.5070/D3265048772

Prabha N, Arora RD, Ganguly S, Chhabra N. Gentian violet: revisited. Indian Journal of Dermatology Venereology and Leprology. 2020;86:600-603. Available from: doi: 10.4103/ijdvl.IJDVL_579_19.

Ünlü K, Askünger A, Söker S, Kilinç N, Karaca C, Erdinc M. Gentian violet solution for staining the previous capsule. Indian Journal of Dermatology Venereology and Leprology. 2020;86(5):600-603. Available from: doi: 10.1016/s0886-3350(00)00360-6.

Moharana B, Singh P, Patel S, Srivastava P, Sharma B. Commentary: High fidelity and cost-effective cataract surgery training system: need of the hour. Indian Journal of Ophthalmology. 2021;69(10):2686-2687. doi: 10.4103/ijo.IJO_1877_21.

(28) Fernandez-Bueno I, Usategui-Martín R, Pastor JC, Andrés-Iglesias C. Ex-vivo method to quantifiably the staining effectiveness of anterior lens capsule dyes. Translational Vision Science & Technology. 2021;10(17):1-6. Available from: 10.1167/tvst.10.14.17.

(29) Naik MP, Sethi H, Kasiviswanathan P. Modified bandage-contact-lens used as a guide-marker for performing continuous curvilinear capsulorhexis by a first year post graduate ophthalmology resident. American Journal of Ophthalmology Case Reports. 2020; 20:100889. Available from: doi: 10.1016/j.ajoc.2020.100889.

(30) Plummer EC. Equine ophthalmology. In: Gellat K. (org.). Veterinary Ophthalmology. 6th ed. New York: Wiley-Blackwell; 2021. Available from: https://www.wiley.com/en-us/Veterinary+Ophthalmology,+2+Volume+Set,+6th+Edition-p-9781119441830.