Specular microscopy of the different regions of the cornea in enucleated swine eyes - ex vivo evaluation

Eduarda Valim Borges de Vargas; Anita  Marchionatti Pigatto; Rafaella Silva Rocha; Maria  Eduarda Mattos Franceschini; João Antonio Tadeu Pigatto

doi:10.1590/1809-6891v24e-75138E

Autores

Eduarda Valim Borges de Vargas Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-7499-0866
Anita Marchionatti Pigatto Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-0521-7984
Rafaella Silva Rocha Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0001-8856-8239
Maria Eduarda Mattos Franceschini Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-6705-2740
João Antonio Tadeu Pigatto Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-1541-9133

DOI:

https://doi.org/10.1590/1809-6891v24e-75138E

Resumo

O objetivo do presente estudo foi avaliar a densidade endotelial e a hexagonalidade das células endoteliais nas diferentes regiões da córnea de suínos utilizando a microscopia especular de contato. Foram estudados 24 bulbos oculares de 12 suínos (Sus scrofa domesticus), machos, com seis meses de idade e da raça Large White. A microscopia especular de contato foi realizada nas regiões central, superior, inferior, lateral e medial. A densidade endotelial média na região central foi de 1865 células/mm², na região superior foi de 1877 células/mm², na região inferior foi de 1854 células/mm², na região lateral foi de 1847 células/mm² e na região medial foi de 1831 células/mm². Na região central, a hexagonalidade foi de 53%, na região superior foi de 54%, na região inferior foi de 54%, na região lateral foi de 54%, na região medial foi de 54%. Não foram observadas diferenças significativas na densidade celular e na hexagonalidade nas diferentes regiões da córnea analisadas. Este estudo demonstrou que a densidade endotelial e a hexagonalidade da área central da córnea representam todo o mosaico endotelial.
Palavras-chave: córnea; endotélio; morfologia; contagem celular; suíno.

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