Estrus response patches, timing for artificial insemination, and GnRH protocol in Split Timed AI beef cattle

Danilo Demeterco; Ryon  S. Walker; Luiz Ernandes Kozicki; Vitor Mohad Valle; A.K. Edwards; J.E. Anderson; Cathleen Collet Williams

doi:10.1590/1809-6891v24e-75115E

Autores

Danilo Demeterco LSU AgCenter, Louisiana, United States https://orcid.org/0000-0002-9556-6095
Ryon S. Walker McGregor Research Center, Texas, United States e Texas A & M AgriLife Research, Texas, United States https://orcid.org/0000-0001-8001-1938
Luiz Ernandes Kozicki Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, Paraná, Brasil https://orcid.org/0000-0002-3700-1811
Vitor Mohad Valle Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, Paraná, Brasil https://orcid.org/0000-0001-5546-5604
A.K. Edwards LSU AgCenter, Louisiana, United States https://orcid.org/0000-0001-8699-3741
J.E. Anderson Southern Cultured Creamery, Pontotoc, Mississippi, United States https://orcid.org/0009-0002-4107-6733
Cathleen Collet Williams LSU AgCenter, Louisiana, United States https://orcid.org/0000-0002-7348-7011

DOI:

https://doi.org/10.1590/1809-6891v24e-75115E

Resumo

Um adesivo de detecção de estro foi usado como ferramenta para determinar o momento ideal para a inseminação artificial em tempo dividido (STAI) e a necessidade de injeção de hormônio liberador de gonadotrofinas (GnRH) no 7º dia do protocolo CO-Sinc + CIDR sem comprometer as taxas de prenhez. As vacas eram cruzadas, multíparas e lactantes (Angus x Charolês, n=216) e foram estratificadas por idades (5,9 2.5 anos), BW (581 67kg), BCS (5,3 0,8; 1-9), intervalo entre partos (78,5 15,5 dias). O grupo de tratamento CTRL = IAT (n=67) foi inseminado após 72 h após a remoção do CIDR; já no grupo tratamento TRT= IATP (n=149) as vacas foram inseminadas 72 ou 84 h após a remoção do CIDR. Todas as fêmeas receberam GnRH (100 mcg I.M.), mais um CIDR (1,38 g de progesterone) no D0, no D7 foi realizado a retirada do CIDR, aplicação de PGF2α (25 mcg I.M.) e colocação do adesivo detector de cio. Após 72 h da remoção do CIDR, uma pontuação foi atribuída ao adesivo (OS1<50% removido; OS2> 50% removido) de todas as fêmeas. As vacas do grupo CTRL receberam a segunda injeção de GnRH (100 mcg I.M.) às 72 h na IAT. Vacas do grupo TRT com OS2 não receberam GnRH. Às 84 h as vacas restantes do grupo TRT receberam um segundo OS, aquelas com OS1 receberam GnRH (100 mcg I.M.) e as vacas com OS2 não receberam GnRH. Amostras de sangue para concentração de progesterona foram coletadas nos D-11 e D-0 para determinar o percentual de vacas ciclando. Os dados foram analisados utilizando-se o Proc Genmod, tendo o tratamento e o técnico de IA como efeitos fixos, o touro como efeito aleatório e o BW, BCS, idade e IBP como covariáveis. Resultados: as taxas de gravidez da IAT foram semelhantes (P= 0,81) entre os grupos CTRL (45,6%) e TRT (44,8%). As taxas de prenhez tenderam a ser maiores (P=0,07) para vacas com OS2 (50,3%) do que para aquelas com OS1 (29,4%). No entanto, ao estender a IAT para 84 horas em vacas não responsivas, 82,0% das vacas TRT não receberam uma segunda aplicação de GnRH na IAT. Concluiu-se que os adesivos detectores de estro reduziram a porcentagem de vacas que necessitaram de GnRH na IAT sem comprometer as taxas de prenhez. Os adesivos de detecção de estro reduziram significativamente o número de vacas que receberam uma segunda injeção de GnRH na IAT.
Palavras-chave: inseminação em tempo dividido; STAI; GnRH; vaca de corte; detecção de cio.

Downloads

Não há dados estatísticos.

Referências

Foote, RH. The history of artificial insemination: Selected notes and notables. Journal of Animal Science. 2002; 80, 1-10. (https://doi.org/10.2527/animalsci2002.80e-suppl_21a)

Dick, M, Silva, MA, Dewes, H. Mitigation of environmental impacts of beef cattle production in southern Brazil – Evaluation using farm-based life cycle assessment. Journal of Cleaner Production. 2015; 87, 58–67. (https://doi.org/10.1016/j.jclepro.2014.10.087)

Troy, SM, Duthie, CA, Ross, DW, Hyslop, JJ, Roehe, R, Waterhouse, A, Rooke, JA. A comparison of methane emissions from beef cattle measured using methane hoods with those measured using respiration chambers. Animal Feed Science Technology. 2016; 211, 227–240. (https://doi.org/10.1016/j.anifeedsci.2015.12.005)

Parlasca, MC, Qaim, M. Meat consumption and sustainability. Annual Review of Resources Economics. 2022; 14:17-41.

Burns, BM, Fordyce, G, Holroyd, RG. A review of factors that impact on the capacity of beef cattle females to conceive, maintain a pregnancy and wean a calf—Implications for reproductive efficiency in northern Australia. Animal Reproduction Science. 2010; 122(1), 1–22. (https://doi.org/10.1016/j.anireprosci.2010.04.010)

Souza, AB, Segui, MS, Kozicki, LE, Weiss, RR, Bertol, MAF, Oliveira, DAM, Abreu, ACMR. Impact of Equine Chorionic Gonadotropin Associated with Temporary Weaning, Estradiol Benzoate, or Estradiol Cypionate on Timed Artificial Insemination in Primiparous Bos Indicus Cows. Brazilian Archive of Biology and Technology. 2016; 59. (https://doi.org/10.1590/1678-4324-2016150389)

Nebel, RL, Dransfield, MG, Jobst, SM, Bame, JH. Automated electronic systems for the detection of oestrus and timing of AI in cattle. Animal Reproduction Science. 2000; 60–61, 713–723. (https://doi.org/10.1016/s0378-4320(00)00090-7)

Baruselli, PS, Sales, JNS, Sala, RV, Vieira, LM, Sá Filho, MF. History, evolution and perspectives of timed artificial insemination programs in Brazil. Animal Reproduction. 2012; 9(3),139-152.

Lamb, GC, Mercadante, VRG, Henry, DD, Fontes, PLP, Dahlen, CR, Larson, JE, DiLorenzo, N. Invited Review: Advantages of current and future reproductive technologies for beef cattle production. The Professional Animal Scientist. 2016; 32(2), 162–171. (https://doi.org/10.15232/pas.2015-01455)

Hill, SL, Grieger, DM, Olson, KC, Jaeger, JR, Dahlen, CR, Bridges, GA, Dantas, F, Larson, JE, Muth-Spurlock, AM, Ahola, JK, Fischer, MC, Perry, GA, Larimore, EL, Steckler, TL, Whittier, WD, Currin, JF, Stevenson, JS.Using estrus-detection patches to optimally time insemination improved pregnancy risk in suckled beef cows enrolled in a fixed-time artificial insemination program. Journal of Animal Science. 2016; 94, 3703-3710. (https://doi.org/10.2527/jas.2016-0469)

Hill, SL, Grieger DM, Olson, KC, Jaeger, JR, Dahlen, CR, Crosswhite, MR, Negrin Pereira, N, Underdahl, SR, Neville, BW, Ahola, J, Fischer, MC, Seidel, GE, Stevenson, JS. Gonadotropin-releasing hormone increased pregnancy risk in suckled beef cows not detected in estrus and subjected to a split-time artificial insemination program. Journal of Animal Science. 2016; 94, 3722-3728. (https://doi.org/10.2527/jas.2016-0582)

Bishop, BE, Thomas, JM, Abel, JM, Poock, SE, Ellersieck, MR, Smith, MF, Patterson, DJ. Split-time artificial insemination in beef cattle: III. Comparing fixed-time artificial insemination to split-time artificial insemination with delayed administration of GnRH in postpartum cows. Theriogenology. 2017; 99, 48-52. (https://doi.org/10.1016/j.theriogenology.2017.04.046)

Stevenson, JS, Hill, SL, Grieger, DM, Olson, KC, Jaeger, JR, Ahola, JK, Seidel, GE, Kasimanickam, RK. Two split-time artificial insemination programs in suckled beef cows. Journal of Animal Science. 2017; 95, 5105-5111. (https://doi.org/10.4148/2378-5977.7557)

Bishop, BE, Thomas, JM, Abel, JM, Poock, SE, Ellersieck, MR, Smith, MF, Patterson, DJ. Split-time artificial insemination in beef cattle: Using estrous response to determine the optimal time(s) at which to administer GnRH in beef heifers and postpartum com Theriogenology. 2016 86(4),1102-1110. (https://doi.org/10.1016/j.theriogenology.2016.03.043 )

Ketchum, JN, Bonacker, RC, Andersen,CM, Smith, EG, Stoecklein, KS, Spinka, CM, JM, Thomas, JM. Evaluation of later timepoints for split-time artificial insemination when using sex-sorted semen among beef heifers following the 14-d CIDR®-PG protocol. Animal Reproduction Science. 2021; 224, 106649

Knickmeyer, ER, Thomas, JM, Locke, JWC, Bonacker, RC, Ellersieck, MR, Poock, SE, Smith, MF, Patterson, DJ, Evaluation of split-time artificial insemination following administration of a long or short-term progestin-based estrus synchronization protocol in beef heifers. Theriogenology. 2019; 133, 179–186. https://doi.org/10.1016/j.theriogenology.2019.04.011.

Abraham, GE, Swerdloff, R, Tulchinsky, D, Odell, WD. Radioimmunoassay of Plasma Progesterone. The Journal of Clinical Endocrinol & Metabolism. 1971; 32(5), 619–624. (https://doi.org/10.1210/jcem-32-5-619)

Pereira, MHC, Wiltbank, MC, Vasconcelos, JLM. Expression of estrus improves fertility and decreases pregnancy losses in lactating dairy cows that receive artificial insemination or embryo transfer. Journal of Dairy Science. 2016; 99(3), 2237–2247. (https://doi.org/10.3168/jds.2015-9903)

Stegner, JE, Bader, JF, Kojima, FN, Ellersieck, MR, Smith, MF, Patterson, DJ. Fixed-time artificial insemination of postpartum beef cows at 72 or 80 h after treatment with the MGA® Select protocol. Theriogenology. 2004; 61(7-8), 1299–1305. (https://doi.org/10.1016/j.theriogenology.2003.07.023)

Lamb, GC, Larson, JE, Geary, TW, Stevenson, JS, Johnson, SK, Day, ML, Landblom, DG. Synchronization of estrus and artificial insemination in replacement beef heifers using gonadotropin-releasing hormone, prostaglandin F 2α, and progesterone. Journal of Animal Science. 2006; 84(11), 3000–3009. (https://doi.org/10.2527/jas.2006-220)

Bishop, BE. Timing GnRH administration with split-time artificial insemination following administration of CIDR-based protocols to synchronize estrus and ovulation in beef heifers and cows. Master’s Thesis. Retrieved from University of Missouri – Columbia. 2015. (https://mospace.umsystem.edu/xmlui/handle/10355/48583)

Walker, RS., Buttrey, B, Gurie, J, Gentry, GT. Evaluating heat detector patch response to determine gonadotropin-releasing hormone use at timed artificial insemination on pregnancy rates in beef cattle. The Professional Animal Scientist. 2014; 30(6), 613–619. (https://doi.org/10.15232/pas.2014-01324)

Geary, TW, Whittier, JC. Effects of timed insemination following synchronization of ovulation using the Ovsynch or CO-Synch protocol in beef cows. The Professional Animal Scientist. 1998; 14, 217-220. (https://doi.org/10.15232/s1080-7446(15)31832-5)

Pursley, JR, Mee, MO, Wiltbank, MC. Synchronization of ovulation in dairy cows using PGF2α and GnRH. Theriogenology. 1995; 44, 915-923. (https://doi.org/10.1016/0093-691x(95)00279-h)

Larson, JE, Lamb, GC, Stevenson, JS, Johnson, SK, Day, ML, Geary, TW, Kesler, DJ, DeJarnette, JM, Schrick, FN, DiCostanzo, A, Arsenau, JD. Synchronization of estrus in suckled beef cows for detected estrus and artificial insemination and timed artificial insemination using gonadotropin-releasing hormone, prostaglandin F2α and progesterone. Journal of Animal Science. 2006; 84, 332-342. (https://doi.org/10.2527/2006.842332x)

Walker, RS, Burns, PD, Whittier, JC, Sides, GE, Zalesky, DD. Evaluation of Gonadotropin-Releasing Hormone and Insemination Time Using the CO-Synch Protocol in Beef Cows. The Professional Animal Scientist. 2005; 21(3), 190–194. (https://doi.org/10.15232/s1080-7446(15)31201-8)

Howard, JM, Falk, DG, Carnahan, KG, Dalton, JC, Chebel, RC, Ahmadzadeh, A. The Use of Gonadotropin- Releasing Hormone in a Progesterone-Based Timed Artificial Insemination Protocol in Replacement Beef Heifers. The Professional Animal Science. 2009; 25(6), 757–761. (https://doi.org/10.15232/s1080-7446(15)30785-3)