Boas práticas na criação e manutenção de zebrafish (Danio rerio) em laboratório no Brasil

Mateus Tavares Kütter; Leonardo Jose Gil Barcellos; Robert Tew Boyle; Luis Fernando Marins; Tony Leandro Rezende da Silveira

doi:10.1590/1809-6891v24e-74134E

Autores

Mateus Tavares Kütter Universidade Federal de Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-2764-7600
Leonardo Jose Gil Barcellos Universidade de Passo Fundo (UPF), Passo Fundo, Rio Grande do Sul, Brasil https://orcid.org/0000-0002-4637-3377
Robert Tew Boyle Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brasi https://orcid.org/0000-0002-1016-2738
Luis Fernando Marins Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brasi https://orcid.org/0000-0002-7219-5295
Tony Leandro Rezende da Silveira Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brasi https://orcid.org/0000-0001-8802-5701

DOI:

https://doi.org/10.1590/1809-6891v24e-74134E

Resumo

As Boas Práticas de Laboratório (BPL) são um sistema de controle de qualidade gerencial que abrange o processo organizacional e as condições sob as quais os estudos não clínicos de saúde e meio ambiente são desenvolvidos. Conforme a Organização Mundial da Saúde (OMS) as BPL devem conter cinco tópicos: recursos, caracterização, regras, resultados e controle de qualidade. O objetivo deste trabalho foi apresentar uma revisão conforme o padrão da OMS para a implementação das BPL em biotério de zebrafish. Considerando que a promoção da saúde única (animal, humana e ambiental) associada a um plano de educação, protocolos e registros são fundamentais para garantir a segurança e a integridade dos trabalhadores/pesquisadores, animais e meio ambiente assim como confiabilidade nos resultados gerados. De certa forma o Brasil ainda necessita de melhorias relacionadas ao bem-estar de organismos aquáticos (leis nacionais, acordos internacionais, programas corporativos e outros); especialmente em relação à utilização destes na pesquisa e desenvolvimento tecnológico. Desta forma, a implementação de BPL fornece uma orientação valiosa para a melhoria do bem-estar animal, e segurança do trabalhador vindo a facilitar a padronização da pesquisa.
Palavras-chave: Danio rerio; padronização; bem-estar; diretrizes reguladoras; legislação.

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