Adição de sucos de laranja, abacaxi e beterraba em diluidor para criopreservação de sêmen de carneiros

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DOI:

https://doi.org/10.1590/1809-6891v24e-72745E

Resumo

Em busca de melhorias na criopreservação do sêmen, substâncias naturais são comumente estudadas com o objetivo de melhorar a qualidade do sêmen. O objetivo deste estudo foi avaliar o efeito da adição de sucos de laranja, abacaxi e beterraba em diferentes concentrações e combinações ao diluidor de criopreservação de sêmen ovino. Foram utilizados cinco ejaculados de cinco carneiros adultos. O pool de sêmen foi diluído em diluente à base de gema de ovo e misturado com os seguintes 15 tratamentos (na concentração final de 400x10⁶ sptz/ml): laranja 10% (O10) e 15% (O15); abacaxi 10% (P10) e 15% (P15); beterraba 10% (B10) e 15% (B15); abacaxi + laranja 10% (PO10) e 15% (PO15); abacaxi + beterraba 10% (PB10) e 15% (PB15); beterraba + laranja 10% (BO10) e 15% (BO15); abacaxi + beterraba + laranja 10% (PBO10) e 15% (PBO15); e o grupo controle (CON). Pós-descongelação em palhetas de 0,25 ml a análise da qualidade do sêmen criopreservado foi realizada pelo CASA e citometria de fluxo. A análise de variância foi realizada e as médias comparadas pelo teste SNK. O teste de correlação de Pearson também foi realizado. Nenhum efeito foi observado na adição de sucos ao diluidor de sêmen antes da criopreservação. Após o descongelamento, embora estatisticamente semelhante ao grupo controle, a motilidade total do grupo B10 atingiu padrões aceitáveis ​​de motilidade total. Além disso, o grupo B10 apresentou os maiores valores (p<0,05) de motilidade progressiva que o grupo controle ou os outros tratamentos. A adição de 10% de suco de beterraba ao diluente de sêmen ovino pode melhorar a criopreservação da motilidade espermática.
Palavras-chaves: antioxidante; congelamento; sêmen; diluidor de sêmen

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Publicado

2022-12-19

Como Citar

BOZZI, A. da R.; PARTICELLI, L. H.; VIANA, C. H. C.; QUIRINO, C. R.; ANDRADE, A. F. C. de A.; FREITAS, F. V. de; PASSARELLI, M. da S.; CELEGHINI, E. C. de C.; BEDOYA, H. J. N.; CHAY-CANUL, A. J.; COSTA, R. L. D. da. Adição de sucos de laranja, abacaxi e beterraba em diluidor para criopreservação de sêmen de carneiros. Ciência Animal Brasileira / Brazilian Animal Science, Goiânia, v. 24, 2022. DOI: 10.1590/1809-6891v24e-72745E. Disponível em: https://revistas.ufg.br/vet/article/view/72745. Acesso em: 22 mar. 2025.

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MEDICINA VETERINÁRIA