Adição de sucos de laranja, abacaxi e beterraba em diluidor para criopreservação de sêmen de carneiros
DOI:
https://doi.org/10.1590/1809-6891v24e-72745EResumo
Em busca de melhorias na criopreservação do sêmen, substâncias naturais são comumente estudadas com o objetivo de melhorar a qualidade do sêmen. O objetivo deste estudo foi avaliar o efeito da adição de sucos de laranja, abacaxi e beterraba em diferentes concentrações e combinações ao diluidor de criopreservação de sêmen ovino. Foram utilizados cinco ejaculados de cinco carneiros adultos. O pool de sêmen foi diluído em diluente à base de gema de ovo e misturado com os seguintes 15 tratamentos (na concentração final de 400x10⁶ sptz/ml): laranja 10% (O10) e 15% (O15); abacaxi 10% (P10) e 15% (P15); beterraba 10% (B10) e 15% (B15); abacaxi + laranja 10% (PO10) e 15% (PO15); abacaxi + beterraba 10% (PB10) e 15% (PB15); beterraba + laranja 10% (BO10) e 15% (BO15); abacaxi + beterraba + laranja 10% (PBO10) e 15% (PBO15); e o grupo controle (CON). Pós-descongelação em palhetas de 0,25 ml a análise da qualidade do sêmen criopreservado foi realizada pelo CASA e citometria de fluxo. A análise de variância foi realizada e as médias comparadas pelo teste SNK. O teste de correlação de Pearson também foi realizado. Nenhum efeito foi observado na adição de sucos ao diluidor de sêmen antes da criopreservação. Após o descongelamento, embora estatisticamente semelhante ao grupo controle, a motilidade total do grupo B10 atingiu padrões aceitáveis de motilidade total. Além disso, o grupo B10 apresentou os maiores valores (p<0,05) de motilidade progressiva que o grupo controle ou os outros tratamentos. A adição de 10% de suco de beterraba ao diluente de sêmen ovino pode melhorar a criopreservação da motilidade espermática.
Palavras-chaves: antioxidante; congelamento; sêmen; diluidor de sêmen
Downloads
Referências
Holt WV. Fundamental aspects of sperm cryobiology: the importance of species and individual differences. Theriogenology. 2000; 53:47-58. Available from: https://doi.org/10.1016/S0093-691X(99)00239-3
Bucak MN, Atessahin A, Yuce A. Effect of anti-oxidants and oxidative stress parameters on ram semen after the freeze-thawing process. Small Ruminant Research. 2008. 75:128-134. Available from: https://doi.org/10.1016/j.smallrumres.2007.09.002
Castelo TS, Frota TR, Silva AR. Considerações sobre a criopreservação do sêmen de caprinos. Acta Veterinaria Brasilica. 2008. 2:67-75. Available from: https://doi.org/10.21708/avb.2008.2.3.885
Câmara DR, Silva SV, Almeida FC, Nunes JF, Guerra MMP. Effects of antioxidants and duration of pre-freezing equilibration on frozen-thawed ram semen. Theriogenology. 2011. 76:341-350. Available from: https://doi.org/10.1016/j.theriogenology.2011.02.013
Aitken RJ, Baker MA. Oxidative stress and male reproductive biology. Reproduction, Fertility and Development., 2004. 16:581-588. Available from: https://doi.org/10.1071/RD03089
Savi PAP, Zavarez LB, Kipper BH, Feliciano MAR, Vicente WRR, Oliveira MEF. Uso de antioxidantes em meios diluidores para sêmen ovino: revisão de literatura. Ars Veterinaria, 2015. 31(1):12-18. Available from: http://dx.doi.org/10.15361/2175-0106.2015v31n1p12-18
Anwar F, Ali M, Hussain AI, Shahid M. Antioxidant and antimicrobial activities of essential oil and extracts of fennel (Foeniculum vulgare MILL.) seeds from Pakistan. Flavour Fragance Journal, 2009. 24:170-176. Available from: https://doi.org/10.1002/ffj.1929
Zhong R, Zhou D. Oxidative stress and role of natural plant derived antioxidants in animal reproduction. Journal of Integrative Agriculture, 2013. 12:1826-1838. Available from: https://doi.org/10.1016/S2095-3119(13)60412-8
Aboagla EME, Terada T. Effects of egg yolk during the freezing step of cryopreservation on the viability of goat spermatozoa. Theriogenology, 2004. 62:1160-1172. Available from: https://doi.org/10.1016/j.theriogenology.2004.01.013
Gardner PT, White TAC, Mcphai DB, Duthie GG. The relative contributions of vitamin C, carotenoids and phenolics to the antioxidant potential of fruit juices. Food Chemistry, 2000. 68:471-474. Available from: https://doi.org/10.1016/S0308-8146(99)00225-3
Cutler GJ, Nettleton JA, Ross JA. Dietary flavonoid intake and risk of cancer in postmenopausal women: The Iowa Women's Health Study. International Journal of Cancer, 2008. 123:664-671. Available from: https://doi.org/10.1002/ijc.23564
Vinson JA, Hao Y, Su X, Zubik L. Phenol Antioxidant Quantity and Quality in Foods: Vegetables. Journal of Agriculture and Food Chemistry., 1998. 46:3630-3634. Available from: https://doi.org/10.1021/jf980295o
Mohdaly AA, Sarhan MA, Smetanska I, Mahmoud A. Antioxidant properties of various solvent extracts of potato peel, sugar beet pulp and sesame cake. Journal of the Science of Food and Agriculture, 2010. 90:218-226. Available from: https://doi.org/10.1002/jsfa.3796
National Research Council, NRC. Nutrient Requirements of Small Ruminants: Sheep, Goats, Cervids, and New World Camelids. The National Academies Press, Washington DC, 2007. The Nat. Acad. Press. 2007
Adeyemo OK, Adeyemo OA, Oyeyemi MO, Agbede AS. Effect of semen extenders on the motility and viability of stored African Catfish (Clarias gariepinus) spermatozoa. Journal of Applied Science and Environmental Management, 2007. 11:13-16. Available from: https://doi.org/10.4314/jasem.v11i1.46827
Daramola JO, Adekunle EO, Onagbesan OO, Oke OE, Ladokun, AO, Abiona JA, Abioja MO, Oyewusi IK, Oyewusi JA, Isah OA, Sogunle OM., Adeleke MA. Protective effects pf fruit-juices on sperm viability of west African dwarf goat bucks during cryopreservation. Animal Reproduction, 2016. 13:7-13. Available from: http://dx.doi.org/10.4322/1984-3143-AR726
Pavaneli APP, Recuero S, Chaves BR, Garcia-Bonavila E, Llavanera M, Pinart E, Bonet S, Andrade AFC, Yest M. The presence of seminal plasma during liquid storage of pig spermatozoa at 17ºC modulates ability to elicit in vitro capacitation and trigger acrosomal exocytosis. International Journal of Molecular Sciences, 2020. 21:4520. Available from: https://doi.org/10.3390/ijms21124520
Batissaco L, Arruda RP, Alves MBR, Andrade MT, Lemes KM, Prado-Filho RR, Almeida TG, Andrade AFC, Celeghini ECC. Cholesterol-loaded cyclodextrin is efficient in preserving sperm quality of cryopreserved ram semen with low freezability. Reproductive Biolology, 2020. 20:14-24. Available from: https://doi.org/10.1016/j.repbio.2020.01.002
Vašíček J, Svoradová A, Baláži A, Jurčík A, Macháč M, Chrenek P. Ram semen quality can be assessed by flow cytometry several hours after post-fixation. Zygote, 2021. 29:130-137. Available from: https://doi.org/10.1017/S0967199420000581
Manjunath P. New insights into the understanding of the mechanism of sperm protection by extender components. Animal Reproduction, 2012. 9:809-815. Available from:https://www.animalreproduction.org/article/5b5a6053f7783717068b46cc/pdf/animreprod-9-4-809.pdf
Colégio Brasileiro de Reprodução Animal – CBRA, 2013. Manual para exame andrológico e avaliação de sêmen animal, 3, 104.
Maxwell WMC, Salamon S. Liquid storage of ram semen: a review. Reproduction, Fertility and Development, 1993. 5:613-638. Available from: https://doi.org/10.1071/RD9930613
Motlagh MK, Sharafi M, Zhandi M, Mohammadi-Sangcheshmeh, A, Shakeri M, Soleimani M, Zeinoaldini S. Antioxidant effect of rosemary (Rosmarinus officinalis L) extract in soybean lecithin-based semen extender following freeze-thawing process of ram sperm. Cryobiology, 2014. 69:217-222. Available from: https://doi.org/10.1016/j.cryobiol.2014.07.007
Aitken RJ. Pathophysiology of human spermatozoa. Current Opinion in Obstetrics & Ginecology, 1994. 6:128-135. Available from: https://europepmc.org/article/MED/8193251
Bartoov B, Bar-Sagie D, Mayevsky A. The effect of pH on ram sperm collective motility driven by mitochondrial respiration. International Journal of Androlology, 1980. 3:602-612. Available from: https://doi.org/10.1111/j.1365-2605.1980.tb00148.x
Azevedo HC, Machado R, Simplício AA, Soares AT. Características do sêmen caprino congelado: influência do tipo de palheta e concentração espermática. Rev. Científica Rural, 2000. 5:148-157. Available from: http://www.alice.cnptia.embrapa.br/alice/handle/doc/45681
Wruss J, Waldenberger G, Huermer S, Uygun P, Lanzerstorfer P, Muller U, Hoglinger O, Webhuber J. Compositional characteristics of commercial beetroot products and beetroot juice prepared from seven beetroot varieties grown in Upper Austria. Journal of Food Composition and Analysis, 2015. 42:46-55. Available from: https://doi.org/10.1016/j.jfca.2015.03.005
Fukuhara R, Nishikawa Y. Effects of various substrates on respiration, glycolysis and motility of goat spermatozoa. Japanese. Journal of Zootechnical Science, 1973. 44:271-274. Available from: https://www.jstage.jst.go.jp/article/chikusan1924/44/5/44_5_271/_pdf
Simón BF, Pérez-Ilzarbe J, Hernández T, Gómez-Cordovés C, Estrella I. Importance of phenolic compounds for the characterization of fruit juices. Journal of Agriculture and Food Chemistry, 1992. 40:1531-1535. Available from: https://doi.org/10.1021/jf00021a012
Melo EA, Maciel MIS, Galvão de Lima VLA, Nascimento RJ. Capacidade antioxidante das frutas. Revista Brasileira de Ciencias Farmaceuticas, 2008. 44:193-200. Available from: https://doi.org/10.1590/S1516-93322008000200005
Malo C, Gil R, Cano N, Gonzales V, Luño V. Fennel (Foeniculum vulgare) provides antioxidante protection for boar semen cryopreservation. Andrologia, 2012. 44:710-715. Available from: https://doi.org/10.1111/j.1439-0272.2011.01254.x
Daghigh-Kia H, Olfati-Karaji R, Hoseinkhani A, Ashrafi I. Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation. Spananish Journal of Agriculture Research, 2014. 12(1):98-105. Available from: https://doi.org/10.5424/sjar/2014121-4486
Gil L, Mascaró F, Mur P, Gale I, Silva A, González N, Malo C, Cano R. Freezing ram semen: The effect of combination of soya and rosemary essences as freezing extender on post-thaw sperm motility. 10th Int Cong Spanish Association of Animal Reproduction (AERA), Cáceres (Spain), June 2-5. p: 91. 2010.
Baghshahi H, Riasi A, Mmahdavi AH, Shirazi A. Antioxidant effects of clove bud (Syzygium aromaticum) extract used with different extenders on ram spermatozoa during cryopreservation. Cryobiology, 2014. 69:482-487. Available from: https://doi.org/10.1016/j.cryobiol.2014.10.009
Silva MA, Peixoto GCX, Lima GL, Bezerra JAB, Campos LB, Paiva ALC, Paula VV, Silva AR. Cryopreservation of collared peccaries (Tayassu tajacu) semen using powdered coconut water (ACO-116c) based extender plus various concentrations of egg yolk and glycerol. Theriogenology, 2012. 78:605–611. Available from: https://doi.org/10.1016/j.theriogenology.2012.03.006
Perumal P, Rajkhowa C. Effect of addition of pomegranate (Punica granatum) juice on the liquid storage (5ºC) of mithun (Bos frontalis) semen. Indian Journal of Animal Research, 2014. 49:470-473. Available from: https://doi.org/10.5958/0976-0555.2015.00091.6
El-Sheshtawy RI, El-Nattat WS, Ali A. Effect of clarified tris egg yolk extender supplemented with strawberry juice (Fragaria spp.) on some characteristics of chilled and frozen cattle semen. International Journal of ChemTech. Research, 2016. 9(6):203-207. Available from: https://www.sphinxsai.com/2016/ch_vol9_no6/1/(203-207)V9N6CT.pdf
El-Sheshtawy RI, El-Sisy GA, El-Nattat WS. Effect of pomegranate juice in Tris-based extender on cattle semen quality after chilling and cryopreservation. Asian Pacific Journal of Reproduction, 2016. 5:335-339. Available from: https://doi.org/10.1016/j.apjr.2016.06.001
Bayemi PH, Banla NR, Leinyuy I, Niba AT, Nsongka VM, Mario G, Shamshuddin M. Use of fruits and raffia palm sap (Raffia hookeri) in chilled bull semen extenders in Cameroon. Agricultural and Biological Science Journal, 2015. 1:142-149. Available from: http://files.aiscience.org/journal/article/html/70040066.html
Krishnaiah D, Sarbatly R, Nithyanandam R. A review of the antioxidant potential of medicinal plant species. Food and Bioproducts Process., 2011. 89:217-233. Available from: https://doi.org/10.1016/j.fbp.2010.04.008
Djeridane A, Yousfi M, Nadjemi B, Boutassouma D, Stocker P, Vidal N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry, 2006. 97:654-660. Available from: https://doi.org/10.1016/j.foodchem.2005.04.028
Ferguson LR. Role of plant polyphenols in genomic stability. Mutation Research, 2001. 475:89-111. Available from: https://doi.org/10.1016/S0027-5107(01)00073-2
Drummen GPC, Van Liebergen CM, Den Kamp JAF, Post JA. C11-BODIPY581/591, a oxidation-sensitive fluorescent lipid peroxidation probe: (micro) spectroscopic characterization and validation of methodology. Free Radical Biology and Medicine, 2002. 33(4):473-490. Available from: https://doi.org/10.1016/S0891-5849(02)00848-1
Nazarewicz RR, Bikineyeva A, Dikalov SI. Rapid and specific measurements of superoxide using fluorescence spectroscopy. SLAS Discovery, 2013. 18:498-503. Available from: https://doi.org/10.1177/1087057112468765
Kasai T, Ogawa K, Mizuno K, Nagai S, Uchida Y, Ohta S, Fujie M, Suzuki K, Hirata S, Hoshi K. Relationship between sperm mitochondrial membrane potential, sperm motility, and fertility potential. Asian Journal of Andrology, 2002. 4:97-103. Available from: http://www.asiaandro.com/archive/1008-682x/4/97.htm?mpclwjsbcesrixsb
Grotter LG, Cattaneo L, MarinI PE, Kjelland ME, Ferré B. Recent advances in bovine sperm cryopreservation techniques with a focus on sperm post-thaw quality optimization. Reproduction Domestic Animal, 2019. 54:655-665. Available from: https://doi.org/10.1111/rda.13409
Downloads
Publicado
Como Citar
Edição
Seção
Licença
Copyright (c) 2022 Ciência Animal Brasileira / Brazilian Animal Science

Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
Autores que publicam nesta revista concordam com os seguintes termos:
- Autores mantém os direitos autorais e concedem à revista o direito de primeira publicação, com o trabalho simultaneamente licenciado sob a Licença Creative Commons Attribution que permite o compartilhamento do trabalho com reconhecimento da autoria e publicação inicial nesta revista.
- Autores têm autorização para assumir contratos adicionais separadamente, para distribuição não-exclusiva da versão do trabalho publicada nesta revista (ex.: publicar em repositório institucional ou como capítulo de livro), com reconhecimento de autoria e publicação inicial nesta revista.
- Autores têm permissão e são estimulados a publicar e distribuir seu trabalho online (ex.: em repositórios institucionais ou na sua página pessoal) a qualquer ponto antes ou durante o processo editorial, já que isso pode gerar alterações produtivas, bem como aumentar o impacto e a citação do trabalho publicado (Veja O Efeito do Acesso Livre).