Eritrograma e estresse oxidativo em bovinos confinados alimentados com feno de Brachiaria sp. e suplementados com antioxidantes

Roberta Dias da Silva Cunha; Gustavo Lage Costa; Ulisses Reis Correia Pinto; Juliana Job Serodio Ferezin; Paulo Henrique Jorge da Cunha; Maria Clorinda Soares Fioravanti

doi:10.1590/1809-6891v22e-70611E

Autores

Roberta Dias da Silva Cunha Universidade Federal de Goiás, Goiânia, Goiás, Brasil https://orcid.org/0000-0001-8301-4990
Gustavo Lage Costa Pontifícia Universidade Católica de Goiás (PUC-GO), Goiânia, Goiás, Brasil https://orcid.org/0000-0002-3569-9566
Ulisses Reis Correia Pinto Instituto Federal Goiano - Campus Urutaí/Ceres, Urutaí, Goiás, Brasil https://orcid.org/0000-0001-5226-9659
Juliana Job Serodio Ferezin Universidade Federal de Goiás, Goiânia, Goiás, Brasil https://orcid.org/0000-0002-7734-0439
Paulo Henrique Jorge da Cunha Universidade Federal de Goiás, Goiânia, Goiás, Brasil https://orcid.org/0000-0002-1478-3670
Maria Clorinda Soares Fioravanti Universidade Federal de Goiás, Goiânia, Goiás, Brasil https://orcid.org/0000-0002-4993-5523

DOI:

https://doi.org/10.1590/1809-6891v22e-70611E

Resumo

As Brachiaria sp contêm esporidesminas que podem ser oxidadas por lipoperoxidação e ocasionar estresse oxidativo. No presente estudo foram avaliados os efeitos de diferentes antioxidantes na lipoperoxidação dos eritrócitos de bovinos da raça Nelore, alimentados com feno de Brachiaria sp. O delineamento experimental foi inteiramente casualizado, em que 40 bovinos machos, inteiros, foram divididos, em cinco tratamentos (G1: controle - sem suplementação; G2: suplementação de selênio e vitamina E; G3: suplementação de zinco; G4: suplementação de selênio e G5: suplementação de vitamina E) e alocados em baias de confinamento, por 105 dias. As amostras de plasma heparinizado ou com ácido etilenodiamino tetra-acético (EDTA) foram obtidas a cada 28 dias para avaliação hematológica e de estresse oxidativo (0, 28 56, 84 e 105 dias). No eritrograma foi mensurado a contagem total de eritrócitos, a hemoglobina e o hematócrito (Ht). Para a avaliação do estresse oxidativo, com o objetivo de analisar as características da membrana do eritrócito foram determinadas as substâncias reativas ao ácido tiobarbitúrico (TBARS), glutationa total (GSH-T), glutationa peroxidase (GSH-Px), catalase (CAT) e superóxido dismutase (SOD). Os resultados demonstraram que independente do tratamento não houve estresse oxidativo durante o período do confinamento experimental e que a associação conjunta de selênio e vitamina E na dieta dos bovinos proporcionaram menor incidência de alterações deletérias sobre os eritrócitos.
Palavras-chaves: enzimas antioxidantes; eritrócito; espécies reativas de oxigênio (ROS); lipoperoxidação; Nelore.

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